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  • Chemistry  (5)
  • Electron microscopy
  • PACS. 61.80.Jh Ion radiation effects – 75.60.-d Domain effects, magnetization curves, and hysteresis – 85.70.Ge Ferrite and garnet devices
  • 2000-2004  (1)
  • 1990-1994  (5)
  • 1975-1979  (1)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    High-pressure freezing of soybean nodules leads to an improved preservation of ultrastructure (1992)
    Springer
    Planta 188 (1992), S. 155-163 
    Details
    ISSN: 1432-2048
    Keywords: Bradyrhizobium ; Electron microscopy ; Glycine (root nodules) ; High-pressure freezing ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract High-pressure freezing of chemically untreated nodules of soybean (Glycine max (L.) Merr.), in sharp contrast to chemical fixation and prefixation, appears to preserve the ultrastructure close to the native state. This is supported by the observation that the peribacteroid membrane of high-pressure-frozen samples is tightly wrapped around the bacteroids, a finding that is fully consistent with the current views on the physiology of oxygen and metabolite transport between plant cytosol and bacteroids. In soybean root nodules, the plant tissue and the enclosed bacteria are so dissimilar that conventional aldehyde-fixation procedures are unable to preserve the overall native ultrastructure. This was demonstrated by high-pressure freezing of nodules that had been pre-fixed in glutaraldehyde at various buffer molalities: no buffer strength tested preserved all ultrastructural aspects that could be seen after high-pressure freezing of chemically untreated nodules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216809
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  • 2
    Electronic Resource
    Electronic Resource
    Damage processes in Fe 3 O 4 magnetic insulator irradiated by swift heavy ions. Experimental results and modelisation (2001)
    Springer
    The European physical journal 24 (2001), S. 291-295 
    Details
    ISSN: 1434-6036
    Keywords: PACS. 61.80.Jh Ion radiation effects – 75.60.-d Domain effects, magnetization curves, and hysteresis – 85.70.Ge Ferrite and garnet devices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: The behavior of the magnetic properties of magnetite Fe3O4 irradiated by swift heavy ions is investigated by magnetization measurements. Although there is no induced structural phase transformation, both coercive field and saturation magnetization are sensitive to ion irradiation and exhibit different behaviors depending on the ion fluence range. In the low fluence regime, the coercive field increases, which is evidence for a strong pinning of magnetic domain boundaries by the induced defects. The magnetization shows a decrease in the saturation value and tends to reorient perpendicularly to the ion track axis. At high fluence, the initial magnetic properties of the sample are nearly restored. The changes in the magnitude and the direction of magnetization are interpreted by magnetostrictive effects related to the stress induced by irradiation. A phenomenological model is applied to reproduce the fluence evolution of the saturation magnetization, assuming relaxation of the stress induced around the core of defects of the tracks by overlapping effects at high fluence. The results are compared to those obtained in the case of yttrium iron garnet Y3Fe5O12.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s10051-001-8676-3
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  • 3
    Electronic Resource
    Electronic Resource
    Synthesis of Tri-, Penta-, and Heptapeptides Containing and (R)-2-alkyl-2-amino-3-(methylamino)-propionic acid residue in the central positionThis project was supported by the Swiss National Science Foundation, project No. 20-30770.91 (1994)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 77 (1994), S. 2035-2050 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: By conventional peptide-coupling methods (C to N direction; mixed anhydride, bis(2)-oxooxazolidin-3-yl)phosphinoyl chloride (Bop-Cl), or dicyclohexylcarbodiimide (DCC), 2-amino-2-methyl-3-(methylamino)-propionic acid and 2-amino-2-ethyl-3-(methylamino)propionic acid ( = 2-amino-2-[(methylamino)methyl]butanoic acid) are incorporated in the central position of tri-, penta-, and heptapeptides (see 3-7, 21, and 22). The fragment coupling of the β -amino group of the diamino-acid moiety in a tetrapeptide led to partial epimerization, and thus, two epimeric heptapeptide derivatives were actually obtained (7 and epi-7). The final deprotection to the free heptapeptide (involving a Me3SiI cleavage of BocNH and MeOCONH, a saponification with NaOH, and HPLC purification) gave both the desired product (isopeptide 21), with the β -amino group inside the peptide backbone, and a product (peptide 22) of transpeptidation, with the α-amino group of the diamino acid incorporated and a (methylamino)methyl group as the side chain. Peptide 22 is completely converted to the isopeptide 21 by prolonged treatment with base. The heptapeptide 21 was analyzed by elaborate 2QF-COSY and NOESY NMR measurements in H2O/CD3OD at -5° (Table, Fig.); there is no indication for β -sheet or helical structures, a fact which was also confirmed by CD measurements.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19940770728
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  • 4
    Electronic Resource
    Electronic Resource
    Metal Complexes with Macrocyclic Ligands. Part XXXIIPart XXXI, see [1].. Reactivity studies of the pendant carboxylic group in a macrocyclic Cu2+ complex towards amide formation and its use as a protein-labelling agent (1990)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 73 (1990), S. 149-153 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The Cu2+ complex of 1, having a non-coordinating carboxylic group, can be reacted under the typical conditions of peptide formation with amines such as 2-methylpropylamine or (pyrid-2-yl)methylamine to give, after removal of Cu2+ with CN-, the amides 4 and 5. The Cu2+ ion is of paramount importance since it protects the four amino groups of the macrocycle so that the amide condensation can specifically be done with the exogenous amine It is also shown that the Cu2+ complex of 1 can be covalently attached to bovine serum albumin (BSA), thus opening the possibility to use this compound as a labelling agent for proteins and antibodies.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19900730116
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  • 5
    Electronic Resource
    Electronic Resource
    Zur Verwendung des Tosylaminocarbonyl-Restes als Amino-Schutzgruppe in der PeptidchemieHerrn Prof. Dr. H. Bretschneider zum 70. Geburtstag gewidmet (1975)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 58 (1975), S. 139-146 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Several N-tosylaminocarbonyl amino acids and corresponding di- and tripeptides were prepared in order to investigate the suitability of the tosylaminocarbonyl group as an amino protecting group in peptide chemistry. The group is shown to be selectively cleavable under neutral conditions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19750580119
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  • 6
    Electronic Resource
    Electronic Resource
    Highly instable (GATA)n-containing sequences of the mouse during the cloning process (1991)
    Weinheim : Wiley-Blackwell
    Electrophoresis 12 (1991), S. 153-158 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Mouse DNA fingerprints were obtained by HaeIII digestion of genomic DNA and ingel hybridization with the (GATA)4 oligonucleotide probe. In order to obtain locusspecific probes that hybridize with only one fragment of the (GATA)4 DNA fingerprint, a genomic library of size-selected inserts was constructed using a system of direct subcloning from the phage clones. During the cloning procedure, the phage as well as the plasmid insert DNAs changed primarily within their repetitive DNA but also within adjacent nonrepetitive sequences, as was demonstrated for several clones by in-gel hybridization with the (GATA)4 probe as well as by sequence analysis. Isolated subclones varied within their (GATA)n repeats, resulting in different insert lengths. Several “metastable” as well as stable (GATA)4-positive subclones could be isolated. Also, vector sequences were affected by alterations during the cloning process. These phenomena are discussed within the context of possible mechanisms for cloning artifacts.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150120210
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  • 7
    Electronic Resource
    Electronic Resource
    Oligonucleotide fingerprinting with (CAC)5: Nonradioactive in-gel hybridization and isolation of individual hypervariable loci (1991)
    Weinheim : Wiley-Blackwell
    Electrophoresis 12 (1991), S. 141-146 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The first topic to be treated in this paper is the nonradioactive DNA fingerprinting by means of in-gel hybridization with digoxigenated (CAC)5. Besides the fact that timeconsuming Southern blotting can be avoided, the dried agarose is an excellent matrix to produce background-free nonradioactive DNA fingerprints. There is no tendency of either the oligonucleotide probe or the antibody towards unspecific binding to the dried agarose. Prehybridization and blocking steps are therefore superfluous. Furthermore, we will discuss what effect the degree of crosslinking of the antibodyenzyme conjugates has. The second topic concerns the isolation and characterization of locus-specific probes from a human (CAC)5 fingerprint. The isolation and characterization of one variable probe, by screening complete genomic libraries, is described and discussed. This probe is compared to a hypervariable single-copy probe, isolated from a size-enriched genomic library. The sequence of the repeat flanking locus-specific probe is presented and a semi-specific, adaptor-mediated polymerase chain reaction was designed to amplify (CAC)n/(GTG)n flanking sequences.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150120208
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