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  • Zea mays  (45)
  • nitrogen  (44)
  • Springer  (89)
  • American Association of Petroleum Geologists (AAPG)
  • Nature Publishing Group
  • 2000-2004  (4)
  • 1990-1994  (67)
  • 1980-1984  (18)
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  • Springer  (89)
  • American Association of Petroleum Geologists (AAPG)
  • Nature Publishing Group
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 6 (1993), S. 239-243 
    ISSN: 1432-2145
    Keywords: Zea mays ; Sperm cell ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sperm cells are thought to be quiescent in pollen and activated upon pollen germination. To test this hypothesis, protein, RNA and DNA synthesis were assessed in Zea mays sperm cells at different times after isolation from pollen. Protein synthesis changed with time; while some proteins were found to be constitutive in both 0 and 24 h cells, others were synthesized and some disappeared. Overall, the number of proteins detected at 24 h doubled compared with freshly isolated cells. Incorporation of [3H]leucine in 24 h cells was about 50 times that in freshly isolated cells, and that of [5, 6-3H]uridine, about 7 times. Very low incorporation of [6-3H]thymidine into the cells was detected; there was no difference between freshly isolated and 24 h cells. It is possible that the differences in synthetic activity between freshly isolated and 24-h-old cells might correspond to sperm cell activation during pollen tube growth. If so, these metabolic changes may play an important role in fertilization.
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  • 2
    ISSN: 1573-5036
    Keywords: ammonia volatilization ; denitrification ; grazing ; model ; nitrate leaching ; nitrogen ; simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The model simulates the cycling of N in grassland systems grazed by beef cattle and predicts the annual amount of N in liveweight gain, and the amounts lost through ammonia volatilization, denitrification and leaching, on the basis of fertilizer application and soil and site characteristics. It aims to provide a better understanding of the way in which these various factors interact in their influence on N transformations. The model has been programmed to run on IBM-compatible personal computers and responds rapidly to changes in input parameters. The model has been constructed from the average annual amounts of N passing through various components of the N cycle in ten field systems grazed by beef cattle. The amounts were either measured directly or were calculated from empirical sub-models, assuming a balance between inputs to, and outputs from the soil inorganic N pool. The model is given wide applicability through the inclusion of a mineralization sub-model which is sensitive to soil texture, sward age, previous cropping history, and climatic zone. Another important sub-model determines the partitioning of soil inorganic N to either plant uptake or the processes of loss: the proportion partitioned to plant uptake decreases as the total amount of soil inorganic N increases. Outputs from the model indicate that fertilizer N has a strong influence on ammonia volatilization, denitrification and leaching at a given site but that, over a range of sites with a given rate of fertilizer N, total loss and the proportions lost by the three processes are greatly influenced by the amount of N mineralized by the soil. The model indicates how fertilizer N should be matched with mineralization to limit gaseous and leaching losses and to achieve optimum efficiency of N use in grazing systems.
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  • 3
    ISSN: 1573-5060
    Keywords: resistance ; taxonomy ; maize ; maize weevil ; indigenous landraces ; phenolic acids ; life history components ; indirect selection ; Zea mays ; Sitophilus zeamais
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Accessions representing twenty eight landraces of maize were assessed for susceptibility to the maize weevil, Sitophilus zeamais in standardized resistance tests. Susceptibility parameters such as weight loss of grain, number of insect progeny produced, the Dobie index of susceptibility, and oviposition on grain were found to vary significantly by genotype, with exceptional resistance found in accessions representing the Naltel, Chapalote and Palomero landraces. As in improved genotypes, susceptibility was negatively correlated to phenolic and protein content of the variety tested but positively correlated to moisture content. A detailed analysis of the phenolics revealed the presence of diferulate which may contribute to mechanical resistance of the seed by cross-linking of cell wall hemicelluloses. A canonical discriminant analysis of the resistance data suggests that most of the five landrace groupings are significantly different. The ancient indigenous and prehistoric mestisos groupings are sources of resistant genotypes.
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  • 4
    ISSN: 1573-5044
    Keywords: DNA delivery ; electroporation electrolyte ; heat shock ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 µF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.
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  • 5
    ISSN: 1573-2932
    Keywords: on-site treatment ; fecal coliform bacteria ; nitrate leaching ; nitrogen ; on-site treatment ; septic effluent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Groundwater effluent sample collectors(zero-tension lysimeters) were installed directlybelow the drainfields of three residential onsitetreatment systems in the Clover/Chambers Creek aquiferregion of Pierce County near Tacoma, WA. The use of asplit effluent delivery system from the septic tank,where half the effluent was delivered under pressureto a normal native soil-only filter system and halfwas delivered to a sand filter system, allowed thedirect comparison of the two commonly-utilized septicsystems for treatment levels. Septic tank effluent(from the septic tank) and percolating water (between0.3 and 0.9 m beneath the effluent distributionlines) was collected between May 1991 and April 1994on 30 occasions. Samples were analyzed for fecalcoliform bacteria, nitrate, nitrite, ammonium andtotal reduced (Kjeldahl) nitrogen. Results of thisstudy indicate that the use of sand filters greatlyincreased removal of fecal coliform bacteria and totalnitrogen. Soil-only filter systems had an average of91% removal of fecal coliforms and 47%of total N; whereas sand filter systems had an averageof 99.8% removal of fecal coliforms and 80% of total N.
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  • 6
    ISSN: 1573-5028
    Keywords: gene-specific probes ; glutamine synthetase ; transcript accumulation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maize genome has been shown to contain six glutamine synthetase (GS) genes with at least four different expression patterns. Noncoding 3′ gene-specific probes were constructed from all six GS cDNA clones and used to examine transcript levels in selected organs by RNA gel blot hybridization experiments. The transcript of the single putative chloroplastic GS2 gene was found to accumulate primarily in green tissues, whereas the transcripts of the five putative GS1 genes were shown to accumulate preferentially in roots. The specific patterns of transcript accumulation were quite distinct for the five GS1 genes, with the exception of two closely related genes.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 58 (1980), S. 211-218 
    ISSN: 1432-2242
    Keywords: B-A translocations ; Catalase Mapping ; Structural genes ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary B-A translocations have been used to map the catalase genes, Cat1, Cat2, and Cat3 of Zea mays. Cat1 was found to be on the short arm of chromosome 5, 9.1 map units from brittle endosperm (bt 1). Cat2 was located on chromosome 1S, while Cat3 was located on the distal half of chromosome 1L. There was no linkage between Cat2 and Cat3. The significance of mapping the catalase structural genes is discussed.
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  • 8
    ISSN: 1573-5036
    Keywords: Aflatoxin ; Aspergillus flavus ; Corn ; Fungi Maize ; Mycotoxins ; Naturally-occurring toxins ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aflatoxin contamination of developing corn (Zea mays L.) kernels caused by natural infection byAspergillus flavus Link ex Fries was studied in hybrids developed for the U.S. corn belt and for the southern U.S. and grown at diverse locations in 1977. Planting dates were staggered to examine the effect of crop maturity on infection by the toxin-producing fungus. A broad range of toxin values was observed at harvest; some levels exceeded the highest that had been previously recorded in corn. The highest concentration of aflatoxin B1 detected was 8030 ppb. Levels of toxin differed significantly among planting dates in Florida and Georgia; the second planting date at these locations contained the highest toxin levels. Elevated concentrations of toxin were characteristic of kernel samples from southern locations and southeast Missouri; at these locations samples from hybrids developed for the south had significantly lower levels of toxin than hybrids developed for the corn belt. Ears with heavy insect damage had higher toxin levels than ears with less evidence of insect attack.
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  • 9
    ISSN: 1573-5036
    Keywords: Aflatoxin ; Aspergillus flavus ; Zea mays ; Ostrinia nubilalis ; Mycotoxins ; Naturally-occurring toxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The interaction was studied among: 1) developing maize kernels (Zea mays L.); 2) European Corn Borer (ECB) (Ostrinia nubilalis Hubner); 3) and three fungal species,Aspergillus flavus Lk. ex Fr.,Penicillium oxalcium Currie and Thom, andFusarium moniliforme Sheld. Two hybrids with varying degrees of resistance to ECB stalk damage were grown in Iowa, Georgia, and Missouri in 1980. One-half of the plots were hand-infested with ECB egg masses. Fungal spores of individual isolates or combinations of the three species were introduced into the silk channels of developing ears in designated plots. ECB larvae were subsequently collected from developing ears. A higher incidence ofA. flavus group isolates was observed in ECB larvae collected from ears that had been inoculated withA. flavus than from insects collected from control ears. Although the resistant hybrid exhibited reduced ECB stalk damage compared with the susceptible variety, no consistent pattern of hybrid effect on the association betweenA. flavus and ECB was observed at all three locations. Differences in aflatoxin B1 levels in mature kernels from the three locations ranged from 8 ppb in Iowa samples to 419 ppb in Missouri kernels. Conditions during crop development at the Missouri location were particularly conducive to elevated presence ofA. flavus propagules in ECB larvae, increased ECB-mediated stalk damage, and greater toxin concentration in mature kernels.
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  • 10
    ISSN: 1573-5036
    Keywords: grassland ; leaching ; leaf litter ; macro-organic matter ; mineralization ; nitrogen ; ploughing ; roots ; stubble
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The amounts of organic matter in the stubble, litter, root and soil macro-organic matter fractions of two swards of perennial ryegrass that had received normal applications of either fertilizer or cattle urine were, on average for the four fractions, about 3000, 500, 11,500 and 8,800 kg ha−1. The swards had been established 8 or 15 years previously and each was sampled at intervals over a period of about one year. The amounts of N contained in the four fractions were, on average, 68, 12, 249 and 240 kg ha−1, a total of 569 kg N ha−1. With other swards, increasing rates of application of fertilizer N were found to have little effect on the amounts of organic matter in stubble and roots. Concentrations of N in the organic matter of the stubble and roots, however, increased significantly with increasing rate of fertilizer application, though, with stubble, moderate rates of application had little effect. Assessments based on these data, together with other published information, indicate that the amount of N mineralized from the combined stubble, litter, root and macro-organic matter fractions during the first year after ploughing may range from about 40 kg to at least 360 kg N ha−1 depending on the age of the sward and its recent management. The amount mineralized is likely to increase with age of sward, with increasing rate of fertilizer N and with utilisation by grazing rather than cutting.
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