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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning, characterization, and sequencing of the hemolysin gene from Edwardsiella tarda (1996)
    Springer
    Archives of microbiology 165 (1996), S. 9-17 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsEdwardsiella tarda ; Hemolysin ; Hemolytic ; gene ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hemolysis is a major symptom of diseased eels infected by Edwardsiella tarda. The hemolysin gene of E. tarda strain ET16 was cloned into plasmid pSK and expressed in Escherichia coli. The mol. mass of the functional β-hemolysin was estimated to be approximately 34 kDa by gel filtration and by SDS-PAGE followed by in situ hemolysin activity analysis. The cloned fragment containing the β-hemolysin locus from E. tarda strain ET16 expressed in E. coli was estimated to be 5.3 kb in length; the deduced gene product was identical in mol. mass and properties to the extracellular products of E. tarda strain ET16. The presence of EcoRI and XbaI sites within the β-hemolysin gene of E. tarda was determined from the loss of hemolytic activity in subclones. Analysis of the DNA sequence of a 2,436-bp HaeIII-HindIII fragment that included EcoRI and XbaI sites revealed three ORFs organized as an operon that encoded three predicted polypeptides of 15,874, 7,055, and 34,804 Da. A 34-kDa polypeptide expressing hemolytic activity in cell lysates of the clone DH5α(pETH3E) is very likely the β-hemolysin encoded by the third ORF. The observation that hemolytic activity appeared in the culture medium of E. tarda, but not in that of E. coli strain DH5α(pETH3E) indicates the existence of a mechanism for transporting the hemolysin across the cell envelope in E. tarda that is different from that of E. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050290
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of load on tribological behaviour of carbon-carbon composites (1996)
    Springer
    Journal of materials science 31 (1996), S. 1221-1229 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The effect of load on the tribological behaviour of three different two-dimensional carbon-carbon composites, including polyacrylonitrile (PAN) fibre-pitch matrix, PAN fibre-chemical vapour infiltrated (CVI) matrix, and pitch fibre-resin/CVI hybrid matrix composites has been compared. Results indicated that friction and wear rate variations with slide distance depended on load and the type of composite. The worn surface morphology was categorized into three types (I, II and III). The friction coefficient, wear rate and temperature all increased sharply when the transition from type I to type II occurred. When the powdery type II debris was compacted to form the lubricative type III debris, the friction coefficient and wear rate declined, although never approached their initial type I levels. For all composites, a higher load can accelerate the transition from type I to type II, but impedes the transition from type II to type III. Under 2.4 MPa, the type II morphology was never observed to transform into type III morphology in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353101
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of break-in speed on early stage sliding behaviour of two-dimensional PAN-pitch based carbon-carbon composite (1995)
    Springer
    Journal of materials science 14 (1995), S. 740-743 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253387
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  • 4
    Electronic Resource
    Electronic Resource
    Filter Banks and Neural Network-based Feature Extraction and Automatic Classification of Electrogastrogram (1999)
    Springer
    Annals of biomedical engineering 27 (1999), S. 88-95 
    Details
    ISSN: 1573-9686
    Keywords: Electrogastrography ; Gastric motility ; Stomach ; Filter banks ; Neural networks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Dysrhythmia in gastric myoelectrical activity has been frequently observed in patients with gastric motor disorders and gastrointestinal symptoms. The assessment of the regularity of gastric myoelectrical activity is of great clinical significance. The aim of this study was to develop an automated assessment method for the regularity of gastric myoelectrical activity from the surface electrogastrogram (EGG). The method proposed in this paper was based on the filter bank and neural network. First, the EGG signal was divided into frequency subbands using filter bank analysis. Second, a parameter called the subband energy ratio (SER) was computed for each subband signal. A multilayer perceptron neural network was then used to automatically classify the EGG signal into four categories: bradygastria, normal, tachygastria, and arrhythmia, using the SER as the input. The EGG recording was made using the standard method of electrogastrography by placing electrodes on the abdominal surface. The study was performed in 40 patients with various gastric motor disorders, ten healthy adults, and ten healthy children. The neural network was trained and tested using the EGG data obtained from the patients. The regularity of gastric myoelectrical activity was assessed based on the classification of the minute-by-minute EGG segments. Using the running spectral analysis method as a gold standard, the proposed automated method had an accuracy of 100% for the training set and 97% for the test set. It was concluded that the proposed method provides an accurate and automatic assessment of the regularity of gastric myoelectrical activity from the EGG. © 1999 Biomedical Engineering Society. PAC99: 8780-y, 8717-d, 0705Mh, 0270Hm
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1114/1.151
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  • 5
    Electronic Resource
    Electronic Resource
    Entrainment of Intestinal Slow Waves with Electrical Stimulation Using Intraluminal Electrodes (2000)
    Springer
    Annals of biomedical engineering 28 (2000), S. 582-587 
    Details
    ISSN: 1573-9686
    Keywords: Gastrointestinal Motility ; Intestinal pacing ; Electrical stimulation ; Electromyography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The aim of this study was to investigate whether the intestinal stimulation would be feasible using a less invasive method: intraluminal electrodes. The study was performed in nine healthy hound dogs (15–26 kg). Four pairs of electrodes were implanted on the serosa of the jejunum at an interval of 5 cm with the most proximal pair 35 cm beyond the pylorus. An intestinal fistula was made 20 cm beyond the pylorus. Simultaneous recordings of intestinal myoelectrical activity were made for 2 h in the fasting state from both intraluminal and serosal electrodes. Various pacing parameters were tested. The frequency of the intestinal slow wave recorded from the intraluminal electrodes was identical to that from the serosal electrodes $$\left( {18.78 \pm 0.3{cpm vs 18}{.75} \pm {0}{.3cpm, }r = 0.99} \right)$$ , p 〈 0.001), and so was the percentage of normal 17–22 cycles/min waves (95.8±33.9% vs 98.16±1.33%, r=0.96, p〈0.01).p 〈 0.01). A complete entrainment of the intestinal slow wave was achieved in every dog with electrical stimulation using intraluminal ring electrodes. The effective pacing parameters were pulse width of 70 ms, amplitude of 4 mA and frequency of 1.1 IF (intrinsic frequency). The time required for the entrainment of the intestinal slow wave with intraluminal pacing was 25.0±2.1s. The maximum driven frequency was found to be 1.43±0.01 IF. The results reveal that intraluminal pacing is an effective and efficient method for the entrainment of intestinal slow waves. It may become a potential approach for the treatment of intestinal motor disorders associated with myoelectrical abnormalities. © 2000 Biomedical Engineering Society. PAC00: 8754Dt, 8719Ff, 8717Nn
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1114/1.294
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  • 6
    Electronic Resource
    Electronic Resource
    Study on the effect of supplementation of ironfortified food to Chinese juvenile athletes by nuclear analysis techniques and blood analysis (1996)
    Springer
    Journal of radioanalytical and nuclear chemistry 212 (1996), S. 51-59 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The iron contents in the hair and blood samples of 37 juvenile athletes who were supplemented with 0, 8 and 16 mg Fe/day, respectively, in the food of ferrous gluconatecontaining chocolate for 3 months were determined before and after the supplementation by INAA, SRXRF and blood analysis. The experimental results showed that after supplementation of the iron-fortified food, the normal ferritin level in the blood of the male athletes was attained and the iron content in the hair was increased with supplementation, but both are not in the positive proportion. Most of the female athletes had similar results. It is suggested that supplementation of 8 mg iron/day to juvenile athletes may be desirable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02165451
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  • 7
    Electronic Resource
    Electronic Resource
    Optimized Overcomplete Signal Representation and its Applications to Time-frequency Analysis of Electrogastrogram (1998)
    Springer
    Annals of biomedical engineering 26 (1998), S. 859-869 
    Details
    ISSN: 1573-9686
    Keywords: Spectral analysis ; Matching pursuit ; Stomach ; Gastric motility ; Electrogastrography ; Evolutionary programming ; Signal analysis ; Electrogastrogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The electrogastrogram (EGG) is a surface measurement of gastric myoelectrical activity. The normal frequency of gastric myoelectrical activity in humans is 3 cycles/min. Abnormal frequencies in gastric myoelectrical activity have been found to be associated with functional disorders of the stomach. The aim of this article was, therefore, to develop new time-frequency analysis methods for the detection of gastric dysrhythmia from the EGG. A concept of overcomplete signal representation was used. Two algorithms were proposed for the optimization of the overcomplete signal representation. One was a fast algorithm of matching pursuit and the other was based on an evolutionary program. Computer simulations were performed to compare the performance of the proposed methods in comparison with existing time-frequency analysis methods. It was found that the proposed algorithms provide higher frequency resolution than the short time Fourier transform and Wigner-Ville distribution methods. The practical application of the developed methods to the EGG is also presented. It was concluded that these methods are well suited for the time-frequency analysis of the EGG and may also be applicable to the time-frequency analysis of other biomedical signals. © 1998 Biomedical Engineering Society. PAC98: 8780+s, 0705Kf
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1114/1.69
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  • 8
    Electronic Resource
    Electronic Resource
    Background radioactivity levels in Canadian grains and oil seeds (1995)
    Springer
    Journal of radioanalytical and nuclear chemistry 194 (1995), S. 359-369 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A study has been carried out to determine the levels of radioisotopes in five Canadian grains from pre-(1985) and post-Chernobyl (1986) crop years. In addition, the radioisotope levels were determined in grains from the 1988 crop year consisting of wheats, oats, barley, corn and oil seeds which included flax, sunflower and canola. The grain and oil-seed samples were ashed to reduce their volume, and the gammaemitters137Cs and40K were determined using a high-purity germanium detector and a high-purity germanium well detector. The90Sr activity measurements required further ashing of the samples followed by chemical separations and β-counting of the separated daughter90Y. The results for the 1985, 1986 and 1988 crop years are discussed and compared with results from grain crops obtained during years of atmospheric bomb tests in the 1950's.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02038435
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  • 9
    Electronic Resource
    Electronic Resource
    Prototype algorithm for automated determination of gastric slow wave characteristics (2000)
    Springer
    Medical & biological engineering & computing 38 (2000), S. 49-55 
    Details
    ISSN: 1741-0444
    Keywords: Gastric pacemaker ; Serosal activity analysis ; Gastric slow wave
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract An algorithm for determining the frequency and propagation time of the gastric slow wave has been designed for integration into a demand gastric pacing system. The algorithm analyses the serosal activity in both the time and frequency domains, and the results are compared to produce a conclusion only when the values are within 5% of each other. Thus, the probability of inappropriate intervention is reduced, at the expense of unidentified segments. The system is verified by comparing the conclusions produced by the algorithm with conclusions from hand analysis of seven canine and one human serosal recordings. The algorithm correctly identifies the slow-wave frequency in the distal portion of the stomach for 90% of the segments, while producing no incorrect results. Slow-wave propagation times in the antrum are correctly identified for 84% of the segments, with no incorrect identifications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344688
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  • 10
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    Interactions that determine the assembly of a retinoid X receptor/corepressor complex (2002)
    National Academy of Sciences
    Details
    Publication Date: 2002-04-23
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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