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  • Articles  (2)
  • L. pimpinellifolium  (2)
  • Springer  (2)
  • 2000-2004
  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    RFLP mapping of QTLs conferring salt tolerance during germination in an interspecific cross of tomato (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1133-1144 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsLycopersicon esculentum ; L. pimpinellifolium ; Salt tolerance ; Restriction fragment length polymorphism (RFLP) ; Quantitative trait loci (QTLs) ; Seed germination ; Molecular markers ; Graphical genotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Most cultivars of tomato (Lycopersicon esculentum) are sensitive to salinity during seed germination and at later stages. Genetic resources for salt tolerance have been identified within the related wild species of tomato. The purpose of the present study was to identify quantitative trait loci (QTLs) for salt tolerance during germination in an inbred backcross (BC1S1) population of an interspecific cross between a salt-sensitive tomato breeding line (NC84173, maternal and recurrent parent) and a salt-tolerant Lycopersicon pimpinellifolium accession (LA722). Onehundred and nineteen BC1 individuals were genotyped for 151 restriction fragment length polymorphism (RFLP) markers and a genetic linkage map was constructed. The parental lines and 119 BC1S1 families (self-pollinated progeny of 119 BC1 individuals) were evaluated for germination at an intermediate salt-stress level (150 mM NaCl+15 mM CaCl2, water potential approximately −850 kPa). Germination was scored visually as radicle protrusion at 8-h intervals for 28 consecutive days. Germination response was analyzed by survival analysis and the time to 25, 50, and 75% germination was determined. In addition, a germination index (GI) was calculated as the weighted mean of the time from imbibition to germination for each family/line. Interval mapping, single-marker analysis and distributional extreme analysis, were used to identify QTLs and the results of all three mapping methods were generally similar. Seven chromosomal locations with significant effects on salt tolerance were identified. The L. pimpinellifolium accession had favorable QTL alleles at six locations. The percentage of phenotypic variation explained (PVE) by individual QTLs ranged from 6.5 to 15.6%. Multilocus analysis indicated that the cumulative action of all significant QTLs accounted for 44.5% of the total phenotypic variance. A total of 12 pairwise epistatic interactions were identified, including four between QTL-linked and QTL-unlinked regions and eight between QTL-unlinked regions. Transgressive phenotypes were observed in the direction of salt sensitivity. The graphical genotyping indicated a high correspondence between the phenotypes of the extreme families and their QTL genotypes. The results indicate that tomato salt tolerance during germination can be improved by marker-assisted selection using interspecific variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051002
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  • 2
    Electronic Resource
    Electronic Resource
    RFLP mapping of QTLs conferring salt tolerance during the vegetative stage in tomato (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 235-243 
    Details
    ISSN: 1432-2242
    Keywords: Key words Lycopersicon esculentum ; L. pimpinellifolium ; Salt tolerance ; Vegetative growth ; Restriction fragment length polymorphism (RFLP) ; Quantitative trait loci (QTLs)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Quantitative trait loci (QTLs) contributing to salt tolerance during the vegetative stage in tomato were investigated using an interspecific backcross between a salt-sensitive Lycopersicon esculentum breeding line (NC84173, maternal and recurrent parent) and a salt-tolerant Lycopersicon pimpinellifolium accession (LA722). One hundred and nineteen BC1 individuals were genotyped for 151 RFLP markers and a linkage map was constructed. The parental lines and 119 BC1S1 families (self-pollinated progeny of the BC1 individuals) were evaluated for salt tolerance in aerated saline-solution cultures with the salt concentration gradually raised to 700 mM NaCl+70 mM CaCl2 (equivalent to an electrical conductivity of approximately 64 dS/m and a water potential of approximately −35.2 bars). The two parental lines were distinctly different in salt tolerance: 80% of the LA722 plants versus 25% of the NC84173 plants survived for at least 2 weeks after the final salt concentration was reached. The BC1S1 population exhibited a continuous variation, typical of quantitative traits, with the survival rate of the BC1S1 families ranging from 9% to 94% with a mean of 51%. Two QTL mapping techniques, interval mapping (using MAPMAKER/QTL) and single-marker analysis (using QGENE), were used to identify QTLs. The results of both methods were similar and five QTLs were identified on chromosomes 1 (two QTLs), 3, 5 and 9. Each QTL accounted for between 5.7% and 17.7%, with the combined effects (of all five QTLs) exceeding 46%, of the total phenotypic variation. All QTLs had the positive QTL alleles from the salt-tolerant parent. Across QTLs, the effects were mainly additive in nature. Digenic epistatic interactions were evident among several QTL-linked and QTL-unlinked markers. The overall results indicate that tomato salt tolerance during the vegetative stage could be improved by marker-assisted selection using interspecific variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051229
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