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  • Springer  (1.113)
  • Institute of Physics  (271)
  • PANGAEA
  • 2000-2004  (497)
  • 1995-1999  (889)
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  • 1
    Digitale Medien
    Digitale Medien
    Leishmania infantum Nicolle, 1908 from southern Spain: Characterisation of the strains from human visceral and cutaneous leishmaniasis and from sandflies; with a numerical analysis of the isoenzymatic data (1996)
    Springer
    Systematic parasitology 33 (1996), S. 177-182 
    Details
    ISSN: 1573-5192
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract This study presents the results of the characterisation of 17 strains ofLeishmania by isoenzyme electrophoresis from a focus of leishmaniasis in southern Spain: two from human visceral leishmaniasis, four from human cutaneous leishmaniasis and 11 from sandflies. The 17 strains are grouped in 6 zymodemes characterised by their variability as regards to the electrophoretic mobility of the enzymes MDH, G6PD, NP and ME. Thus, we confirm the high intraspecific variability ofLeishmania (L.) infantum in a focus of southern Spain, as already suggested by previous studies. Zymodemes GR-15 and GR-17 are also described for the first time in Spain, and they characteristically possess the same relative electrophoretic mobility in the enzyme ME (93). Sixteen zymodemes of theL. infantum complex found in southern Spain were numerically analysed on the basis of the enzymatic profiles of 122Leishmania strains characterised from this area.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01531198
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    A Long-Term Blockade of L-Type Calcium Currents Upregulates the Number of Ca2+ Channels in Skeletal Muscle (1999)
    Springer
    The journal of membrane biology 168 (1999), S. 141-148 
    Details
    ISSN: 1432-1424
    Schlagwort(e): Key words: Ca2+ channels — DHP receptors — Upregulation — Skeletal muscle — Channel blockers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract. The effects of a long-term blockade of L-type Ca2+ channels on membrane currents and on the number of dihydropyridine binding sites were investigated in skeletal muscle fibers. Ca2+ currents (I Ca) and intramembrane charge movement were monitored using a voltage-clamp technique. The peak amplitude of I Ca increased by more than 40% in fibers that were previously incubated for 24 hr in solutions containing the organic Ca2+ channel blocker nifedipine or in Ca2+-free conditions. A similar incubation period with Cd2+, an inorganic blocker, produced a moderate increase of 20% in peak I Ca. The maximum mobilized charge (Q max) increased by 50% in fibers preincubated in Ca2+-free solutions or in the presence of Cd2+. Microsomal preparations from frog skeletal muscle were isolated by differential centrifugation. Preincubation with Cd2+ prior to the isolation of the microsomal fraction doubled the number of 3H-PN200-110 binding sites and produced a similar increase in the values of the dissociation constant. The increase in the number of binding sites is consistent with the increase in the peak amplitude of I Ca as well as with the increase in Q max.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002329900504
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Effect of Polyethylene on Osteocalcin, Alkaline Phosphatase and Procollagen Secretion by Human Osteoblastic Cells (1998)
    Springer
    Calcified tissue international 62 (1998), S. 453-456 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Key words: Polyethylene — Osteocalcin — Alkaline phosphatase — Procollagen — Osteoblast.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Abstract. We have studied the direct effects of polyethylene particles on osteoblastic function in primary human bone cell cultures. The cells were obtained from trabecular bone fragments of patients undergoing knee reconstructive surgery. When the cells reached confluency, they were subcultured into two flasks, one untreated (control culture) and the other treated with polyethylene particles, and incubated until confluency. Osteoblastic function was evaluated by assaying osteocalcin, alkaline phosphatase, and C-terminal procollagen type I, with and without 1,25(OH)2D stimulation, in the cell-conditioned medium. We found that addition of polyethylene to these osteoblastic cell cultures induced higher levels of secreted osteocalcin after 1,25(OH)2D stimulation. Alkaline phosphatase levels increased whereas C-terminal procollagen type I levels decreased in the cell conditioned medium after polyethylene was added to the cultures. Treatment of the control cultures with 1,25(OH)2D stimulated alkaline phosphatase levels and decreased C-terminal procollagen type I. However, these osteoblastic markers in 1,25(OH)2D-treated cells did not change in cultures with polyethylene. This study demonstrates that polyethylene particles have a direct effect on osteoblastic markers in human bone cells in culture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002239900459
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  • 4
    Digitale Medien
    Digitale Medien
    Influence of Skeletal Site of Origin and Donor Age on Osteoblastic Cell Growth and Differentiation (1999)
    Springer
    Calcified tissue international 64 (1999), S. 280-286 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Key words: Human osteoblastic cells — Skeletal site — Aging.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Abstract. Bone loss with aging may be due, at least in part, to inadequate bone formation. Moreover, the process of bone aging is known to follow a different pattern throughout the skeleton. In this study, we examined the cell proliferation rate (area under the cell growth curve, AUC) and the secretion of C-terminal type I procollagen (PICP), alkaline phosphatase (ALP), and osteocalcin (OC) in primary cultures of osteoblastic cells from human trabecular bone. Osteoblastic cells were obtained for 168 donors (100 women and 68 men). Ninety-eight bone samples were obtained from subjects undergoing knee arthroplastia, 52 aged 50–70 years (64 ± 5) and 46 over age 70 (73 ± 2). Another 70 bone samples were obtained from subjects undergoing hip arthroplastia; 51 were 50–70 years old (64 ± 4) and 19 were over 70 (75 ± 5). Osteoblastic cells from the older donors had a lower proliferation rate and OC secretion than those from younger subjects. However, ALP secretion was higher in the former subjects, whereas PICP secretion was unchanged. Osteoblastic cells from hip had a lower proliferation rate than those from knee. PICP secretion was also lower and ALP secretion was higher in the former cells. In age-matched cell cultures, osteoblastic cells from the knee had higher proliferation rate and PICP secretion than osteoblastic cells from the hip. However, ALP secretion was lower in knee osteoblastic cells than those from hip only in the younger group. With aging, ALP secretion was found to increase in knee osteoblactic cells, whereas OC secretion decreased in osteoblastic cell cultures from the hip. Our findings suggest that bone loss with aging may be accounted for, at least in part, by a decreased osteoblastic cell proliferation and an increased osteoblastic maturation. In addition, our data indicate that these changes with aging do not occur similarly at different skeletal sites.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002239900619
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    The genital disc of Drosophila melanogaster. (1997)
    Springer
    Development genes and evolution 207 (1997), S. 216-228 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Key words Drosophila ; Genital disc ; engrailed ; Compartment ; Homeotic
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The genital disc of Drosophila, which gives rise to the genitalia and analia of adult flies, is formed by cells from different embryonic segments. To study the organization of this disc, the expressions of segment polarity and homeotic genes were investigated. The organization of the embryonic genital primordium and the requirement of the engrailed and invected genes in the adult terminalia were also analysed. The results show that the three primordia, the female and male genitalia plus the analia, are composed of an anterior and a posterior compartment. In some aspects, each of the three primordia resemble other discs: the expression of genes such as wingless and decapentaplegic in each anterior compartment is similar to that seen in leg discs, and the absence of engrailed and invected cause duplications of anterior regions, as occurs in wing discs. The absence of lineage restrictions in some regions of the terminalia and the expression of segment polarity genes in the embryonic genital disc suggest that this model of compartmental organization evolves, at least in part, as the disc grows. The expression of homeotic genes suggests a parasegmental organization of the genital disc, although these genes may also change their expression patterns during larval development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004270050110
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  • 6
    Digitale Medien
    Digitale Medien
    Bioconversion of lutein to products with aroma (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 528-534 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A residual mud sample from the marigold flower dehydration process was screened and 19 putative colonies were isolated for their ability to degrade lutein in a chemically defined medium supplemented with marigold flower flour as a carbon source. Among the colonies isolated, two generated volatile compounds in fermentation and one was chosen for further study for its ability to produce a strong tobacco smell. This colony contained two microorganisms, identified as Geotrichum sp. and Bacillus sp. The aroma production requires the presence of both microorganisms and lutein. Using gas chromatography coupled to mass spectrometry (GC/MS), four compounds were identified: 7,8-dihydro- β-ionol, β-ionone, 7,8-dihydro-β-ionone, and 3-hydroxy-β-ionone, in proportions of 84.2%, 9.4%, 3.5%, and 2.9%, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530000421
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Cadmium and Lead Contents in Suburban and Urban Soils from Two Medium-Sized Cities of Spain: Influence of Traffic Intensity (2000)
    Springer
    Bulletin of environmental contamination and toxicology 64 (2000), S. 250-257 
    Details
    ISSN: 1432-0800
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001289910037
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Effect of the Nature of Exogenous Organic Matter on Pesticide Sorption by the Soil (1997)
    Springer
    Archives of environmental contamination and toxicology 33 (1997), S. 117-124 
    Details
    ISSN: 1432-0703
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik , Medizin
    Notizen: Abstract. A study was carried out on the sorption of two sparingly water-soluble pesticides (diazinon and linuron) by a sandy loam soil modified with different exogenous organic materials (EOMs) containing humic-like substances: city refuse compost (CRC), peat (P), commercial “humic” acid (HA), liquid “humic” acid (LHA), and two (nonhumic) model compounds (surfactants), tetradecyltrimethylammonium bromide (TDTMA) and sodium dodecyl sulphate (SDS), before and after 2- and 8-month incubation periods with the soil. In all cases, the isotherms fitted the Freundlich sorption equation (x/m = KC e n ), generally with r 2 values greater than 0.99. The value of the sorption constant K for the natural soil was 8.81 for diazinon and 2.29 for linuron. These values increased significantly for EOM modified soils with respect to natural soil, with the exception of the samples modified with SDS and LHA, in which cases they decreased, possibly due to the micellar properties of these compounds. Incubation of EOMs with soil increased their sorption capacity: the K oc values were increased proportionally to the incubation time for both pesticides and for all treatments carried out. Accordingly, the sorption capacity of hydrophobic pesticides increases with the degree of evolution in the soil of EOMs with “humic”-type compounds, possibly due, among other causes, to the increase in the EOMs' colloidal properties and the modifications occurring in the hydrophobic-hydrophilic characteristics of the soil surfaces. The main conclusion is that application to the soil of carbon-rich wastes, especially those with a high degree of maturity, may offer an important strategy for reducing pesticide leaching and for eliminating pesticide residues from soil with the use of anionic surfactants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002449900232
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    On spectral pollution in the finite element approximation of thin elastic “membrane” shells (1997)
    Springer
    Numerische Mathematik 75 (1997), S. 473-500 
    Details
    ISSN: 0945-3245
    Schlagwort(e): Mathematics Subject Classification (1991):65N30
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Summary. The bending terms in a shell are small with respect to membrane ones as the thickness tends to zero. Consequently, the membrane approximation gives a good description of vibration properties of a thin shell. This vibration problem is associated with a non-compact resolvent operator, and spectral pollution could appear when computing Galerkin approximations. That is to say, there could exist sequences of eigenvalues of the approximate problems that converge to points of the resolvent set of the exact problem. We give an account of the state of the art of this problem in shell theory. A description of the phenomenon and its interpretation in terms of spectral families are given. A theorem of localization of the region where pollution may appear is stated and its complete proof is published for the first time. Recipes are given for avoiding the pollution as well as indications on the possibility of a posteriori elimination.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002110050249
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  • 10
    Digitale Medien
    Digitale Medien
    Influence of hydrogen distribution on magnetic properties of amorphous samples (2000)
    Springer
    The European physical journal 14 (2000), S. 473-476 
    Details
    ISSN: 1434-6036
    Schlagwort(e): PACS. 75.80.+q Magnetomechanical and magnetoelectric effects, magnetostriction - 75.60.-d Domain effects, magnetization curves, and hysteresis - 66.30.-h Diffusion in solids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract: We have studied the effect of the homogeneity of hydrogen distribution on magnetic properties of hydrogen charged amorphous samples (Metglass 2826MB: Fe40Ni40Mo4B18). The results suggest that, in high magnetostrictive samples, the induced internal stresses and hence the magnetic anisotropy are related to the hydrogen distribution and not to the total quantity of hydrogen inside the sample. To perform this study, the homogeneity of hydrogen distribution in the sample was improved by using a very low rate of hydrogen charging by means of a pulsed electrolytic current. Furthermore, this pulsed electrolytic current allows us to introduce a high quantity of hydrogen without breaking the sample.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s100510051055
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