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  • 1
    Electronic Resource
    Electronic Resource
    Genetic diversity of small eukaryotes from the coastal waters of Nansha Islands in China (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 240 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Population structures and genetic diversity of the small eukaryotic plankton from the coastal waters of the Nansha Islands in China were investigated. Two genes libraries using 18S rDNA of the marine small eukaryotes were constituted, and 323 clones were identified within alveolates (more than 43%), acanthareas, viridiplantaes, and stramenopiles. Many novel clones were detected in the two libraries, including two groups of alveolates and two clades related to both acanthareas and polycystineas. Several sequences unrelated to any other known eukaryotes may represent early branches in the phylogenetic tree. Our results reveal that there is a high diversity and abundance of small eukaryotes in the marine regions of China.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2004.09.030
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  • 2
    Electronic Resource
    Electronic Resource
    Concentration effects of dicamba on shoot regeneration in wheat (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Shoot formation and regeneration rates of calli derived from immature embryos of three spring wheat varieties were investigated to evaluate the influence on wheat regeneration of different concentrations of dicamba (0.5, 0.1 and 0.02 mg/l) in the regeneration medium. It was found that dicamba concentrations much lower (0.02 and 0.1 mg/l) than the levels reported previously (1 and 0.5 mg/l) induced significantly more shoots per callus in all three varieties, while the enhancement in regeneration rate was variety dependent. Overall, a dicamba concentration of 0.02 mg/l was more favourable for wheat regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00523.x
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  • 3
    Electronic Resource
    Electronic Resource
    Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In an effort to optimize tissue culture responses of turf-type tall fescue for genetic transformation, the effects of five culture medium supplements on tissue culture responses were investigated with immature embryos and mature seeds of an elite cultivar, ‘Coronado’, as explant tissues. For both explants, calli induced on 6-benzylaminopurine (BAP)-containing medium had significantly improved regeneration ability. The optimal concentration of BAP for the induction of regenerable callus from mature seeds was 0.1 mg/l. Thidiazuron also improved callus regeneration frequency of mature seeds. Casein hydrolysate, L-proline and myo-inositol improved callus induction of immature embryos but not mature seeds. They did not improve callus regeneration frequencies with either explant tissue. By simply slicing the mature seeds into two halves longitudinally, the callus induction frequencies, as well as the corresponding overall plant regeneration frequencies, were increased approximately three- to six-fold in all three combinations of 2,4-dichlorophenoxyacetic acid and BAP in callus induction media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00594.x
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro somatic embryogenesis in turf-type bermudagrass: roles of abscisic acid and gibberellic acid, and occurrence of secondary somatic embryogenesis (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Bermudagrass is an important warm-season turfgrass species that is recalcitrant in regeneration in tissue culture. In a previous report, we observed that somatic embryogenesis of immature inflorescence culture was substantially improved when low levels of 2,4-dichloro-phenoxy acetic acid (1 mg/l) and 6-benzylaminopurine (BAP, 0.01 mg/l) were included in the callus induction medium. The object of this study was to further improve the culture conditions to enhance somatic embryo formation and plantlet regeneration. It was shown that the abscisic acid supplement (2 or 5 mg/l) to the above callus induction medium further enhanced somatic embryogenesis in hybrid bermudagrass (Cynodon dactylon×Cynodon transvaalensis) cv. ‘Tifgreen’. The addition of gibberellic acid (0. 2 mg/l) to the BAP (1 mg/l)-containing regeneration medium accelerated germination/regeneration of the somatic embryos. Secondary and repetitive somatic embryogenesis, which is rarely reported in monocots, was observed in common bermudagrass (Cynodon dactylon, cv. ‘Savannah’), and a full course of such a development was captured by a periodical microphotography. Scanning electron microscopy further confirmed the observation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00684.x
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  • 5
    Electronic Resource
    Electronic Resource
    Phosphorylation of the Nicotinic Acetylcholine Receptor by Protein Tyrosine Kinases (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 757 (1995), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1995.tb17476.x
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  • 6
    Electronic Resource
    Electronic Resource
    Limited Heterogeneity in T-Cell Receptor Vβ Chain Gene Expression in Arthritic Joints of BUB/BnJ (H-2q) Mice—A T-Cell Receptor Vβa Strain (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 756 (1995), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1995.tb44517.x
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation and Certification of Re-Os Dating Reference Materials: Molybdenites HLP and JDC (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Geostandards and geoanalytical research 28 (2004), S. 0 
    Details
    ISSN: 1751-908X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Two Re-Os dating reference material molybdenites were prepared. Molybdenite JDC and molybdenite HLP are from a carbonate vein-type molybdenum-(lead)-uranium deposit in the Jinduicheng-Huanglongpu area of Shaanxi province, China. The samples proved to be homogeneous, based on the coefficient of variation of analytical results and an analysis of variance test. The sampling weight was 0.1 g for JDC and 0.025 g for HLP. An isotope dilution method was used for the determination of Re and Os. Sample decomposition and pre-concentration of Re and Os prior to measurement were accomplished using a variety of methods: acid digestion, alkali fusion, ion exchange and solvent extraction. Negative thermal ionisation mass spectrometry and inductively coupled plasma-mass spectrometry were used for the determination of Re and 187Os concentration and isotope ratios. The certified values include the contents of Re and Os and the model ages. For HLP, the Re content was 283.8 ± 6.2 μg g−1, 187Os was 659 ± 14 ng g−1 and the Re-Os model age was 221.4 ± 5.6 Ma. For JDC, the Re content was 17.39 ± 0.32 μg g−1, 187Os was 25.46 ± 0.60 ng g−1 and the Re-Os model age was 139.6 ± 3.8 Ma. Uncertainties for both certified reference materials are stated at the 95% level of confidence. Three laboratories (from three countries: PR. China, USA, Sweden) joined in the certification programme. These certified reference materials are primarily useful for Re-Os dating of molybdenite, sulfides, black shale, etc.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1751-908X.2004.tb01042.x
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  • 8
    Electronic Resource
    Electronic Resource
    Diversity and structure of the archaeal community in the leachate of a full-scale recirculating landfill as examined by direct 16S rRNA gene sequence retrieval (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 214 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The diversity and structure of the archaeal community in the effluent leachate from a full-scale recirculating landfill was characterized by direct 16S rRNA gene (16S rDNA) retrieval. Total-community DNA was extracted from the microbial assemblages in the landfill leachate, and archaeal 16S rDNAs were amplified with a universally conserved primer and an Archaea-specific primer. The amplification product was then used to construct a 16S rDNA clone library, and 70 randomly selected archaeal clones in the library were grouped by restriction fragment length polymorphism (RFLP) analysis. Sequencing and phylogenetic analysis of representatives from each unique RFLP type showed that the archaeal library was dominated by methanogen-like rDNAs. Represented in the kingdom of Euryarchaeota were phylotypes highly similar to the methanogenic genera Methanoculleus, Methanosarcina, Methanocorpusculum, Methanospirillum and Methanogenium, where the clone distribution was 48, 11, 3, 1 and 1, respectively. No sequences related to known Methanosaeta spp. were retrieved. Four rDNA clones were not affiliated with the known methanogenic Archaea, but instead, they were clustered with the uncultured archaeal sequences recently recovered from anaerobic habitats. Two chimeric sequences were identified among the clones analyzed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11353.x
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic variation of Cordyceps sinensis, a fruit-body-producing entomopathogenic species from different geographical regions in China (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 230 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cordyceps sinensis is one of the most valuable medicinal fungi in the Orient. It is naturally distributed in the eastern extension area of the Qinghai–Tibet plateau, at an altitude over 4000 m high. In order to investigate genetic variation and evolutionary relationships of C. sinensis from different geographical regions, 17 isolates of C. sinensis were collected from different provinces and the complete sequences of rDNA ITS were determined. On the basis of 5.8S rDNA and ITS region analysis, it was clearly shown that the ITS sequences within C. sinensis are highly homologous regardless of geographical origin. The distance values between the sequences in this study were lower than 0.03. This implied that C. sinensis from different geographic regions are the same species; they are not different species or a species complex. The results also showed that distance values between C. sinensis and Hirsutella sinensis are of the same order as those within C. sinensis from different geographic regions. This confirmed our previous results that C. sinensis should only have H. sinensis as its asexual stage whatever the geographic region from which the samples were collected. An rDNA ITS clone library was established to obtain further evidence for the interpretation of the fungal community structure from C. sinensis and to confirm the accuracy of the taxonomic identities produced by directly sequencing the rDNA ITS region. The discrimination between intraspecies of C. sinensis might provide additional data for the authentication of medicinal material at the species or intraspecies level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00889-9
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  • 10
    Electronic Resource
    Electronic Resource
    On Hypotenuse Diagrams in Ancient China (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Centaurus 39 (1997), S. 0 
    Details
    ISSN: 1600-0498
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0498.1997.tb00031.x
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