Publication Date:
2004-03-06
Description:
CD8 T lymphocytes recognize peptides of 8 to 10 amino acids presented by class I molecules of the major histocompatibility complex. Here, CD8 T lymphocytes were found to recognize a nonameric peptide on melanoma cells that comprises two noncontiguous segments of melanocytic glycoprotein gp100(PMEL17). The production of this peptide involves the excision of four amino acids and splicing of the fragments. This process was reproduced in vitro by incubating a precursor peptide of 13 amino acids with highly purified proteasomes. Splicing appears to occur by transpeptidation involving an acyl-enzyme intermediate. Our results reveal an unanticipated aspect of the proteasome function of producing antigenic peptides.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vigneron, Nathalie -- Stroobant, Vincent -- Chapiro, Jacques -- Ooms, Annie -- Degiovanni, Gerard -- Morel, Sandra -- van der Bruggen, Pierre -- Boon, Thierry -- Van den Eynde, Benoit J -- New York, N.Y. -- Science. 2004 Apr 23;304(5670):587-90. Epub 2004 Mar 4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Ludwig Institute for Cancer Research and Cellular Genetics Unit, Universite de Louvain, B-1200 Brussels, Belgium.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15001714" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Antigen Presentation
;
COS Cells
;
Cell Line, Tumor
;
Cysteine Endopeptidases/*metabolism
;
Electroporation
;
HLA-A Antigens/immunology
;
Humans
;
Interferon-gamma/biosynthesis
;
Melanoma
;
Membrane Glycoproteins/chemistry/*immunology/metabolism
;
Models, Chemical
;
Multienzyme Complexes/*metabolism
;
Neoplasm Proteins/chemistry/*immunology/metabolism
;
Peptide Fragments/*immunology/metabolism
;
Proteasome Endopeptidase Complex
;
Protein Precursors/metabolism
;
*Protein Splicing
;
T-Lymphocytes, Cytotoxic/chemistry/*immunology
;
Transfection
;
gp100 Melanoma Antigen
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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