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  • American Institute of Physics (AIP)  (76)
  • Cambridge University Press  (52)
  • International Union of Crystallography (IUCr)  (50)
  • Annual Reviews
  • Periodicals Archive Online (PAO)
  • 2000-2004  (67)
  • 1995-1999  (111)
  • 1965-1969  (36)
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  • 1
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray diffraction studies of a cobalt-substituted derivative of the iron-dependent alcohol dehydrogenase from Zymomonas mobilis (1996)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 218-220 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The iron-dependent alcohol dehydrogenase from Zymomonas mobilis has been crystallized in a form suitable for X-ray diffraction studies. The crystals grew in hanging drops by vapor diffusion, equilibrating with a solution comprising 25–27% methoxypolyethylene glycol 5000 and 1 mM Co2+ in a 0.2 M succinic acid/potassium hydroxide buffer at pH 5.5–5.7 at 281 K. Crystals are tetragonal, P4122 (or P4322), with unit-cell dimensions a = b = 125.7, c = 248.1 Å. Four molecules comprise the asymmetric unit, and a self-rotation function indicates twofold local symmetry perpendicular to the unique axis and 15° from a crystallographic twofold axis. Diffraction data to 3.0 Å have been collected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444995009103
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  • 2
    Electronic Resource
    Electronic Resource
    Structure of recombinant human lactoferrin expressed in Aspergillus awamori (1999)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 403-407 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Human lactoferrin (hLf) has considerable potential as a therapeutic agent. Overexpression of hLf in the fungus Aspergillus awamori has resulted in the availability of very large quantities of this protein. Here, the three-dimensional structure of the recombinant hLf has been determined by X-ray crystallography at a resolution of 2.2 Å. The final model, comprising 5339 protein atoms (residues 1–691, 294 solvent molecules, two Fe3+and two CO_3^{2-} ions), gives an R factor of 0.181 (free R = 0.274) after refinement against 32231 reflections in the resolution range 10–2.2 Å. Superposition of the recombinant hLf structure onto the native milk hLf structure shows a very high level of correspondence; the main-chain atoms for the entire polypeptide can be superimposed with an r.m.s. deviation of only 0.3 Å and there are no significant differences in side-chain conformations or in the iron-binding sites. Dynamic properties, as measured by B-value distributions or iron-release kinetics, also agree closely. This shows that the structure of the protein is not affected by the mode of expression, the use of strain-improvement procedures or the changes in glycosylation due to the fungal system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444998011226
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  • 3
    Electronic Resource
    Electronic Resource
    Subunit dissociation and reassociation leads to preferential crystallization of haemoglobin Bart's (γ4) from solutions of human embryonic haemoglobin Portland (ζ2γ2) at low pH (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 921-924 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: A variety of human haemoglobins (Hbs) are produced at different stages of human development, including three embryonic Hbs, foetal Hb and adult Hb. All are heterotetramers. During crystallization experiments on human embryonic Hb Portland (ζ2γ2), it was discovered by crystallographic and biochemical analysis that the homotetramer Hb Bart's (γ4) preferentially crystallizes from ζ2γ2 solutions below pH 5. This results from dissociation of Hb Portland into γ2 dimers and ζ monomers and has interesting implications for subunit interactions and tetramer stability in Hbs. It also makes possible a full crystallographic analysis of Hb Bart's, which is of considerable medical significance because of its presence in the red blood cells of millions of people worldwide who suffer from α-thalassaemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444901004516
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  • 4
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray analysis of native and recombinant human bile-salt dependent lipase: strategies for improvement of diffraction quality (2000)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 56 (2000), S. 478-480 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Human bile-salt dependent lipase (BSDL), secreted into both the digestive tract and human milk, is integral to the effective absorption of dietary lipids. In attempts to obtain crystals suitable for high-resolution X-ray crystallographic studies, various forms of the enzyme have been crystallized, including native and desialidated human milk BSDL and both intact recombinant BSDL and a truncated form lacking the heavily glycosylated C-terminal repeat region. Trigonal crystals of native BSDL, with unit-cell parameters a = b = 90.0, c = 156.1 Å, were obtained using 15–20%(w/v) PEG 8000 as precipitant. These crystals diffract to 3.5 Å along the unique axis, but to only 5–7 Å in orthogonal directions. Crystals of recombinant truncated BSDL grown from 15–20%(w/v) PEG 6000 are orthorhombic, space group P212121, with unit-cell parameters a = 59.2, b = 90.0, c = 107.7 Å, and diffract to 2.6 Å resolution. These are suitable for structural analysis by X-ray crystallography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444900000986
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  • 5
    Electronic Resource
    Electronic Resource
    Expression, crystallization and preliminary characterization of methylmalonyl coenzyme A epimerase from Propionibacterium shermanii (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 706-708 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Methylmalonyl-CoA epimerase (MMCE) is an enzyme that interconverts the R and S epimers of methylmalonyl-CoA in the pathway that links propionyl-CoA with succinyl-CoA. This is used for both biosynthetic and degradative processes, including the breakdown of odd-numbered fatty acids and some amino acids. The enzyme has been expressed in Escherichia coli both as the native enzyme and as its selenomethionine (SeMet) derivative. Crystals of both forms have been obtained by vapour diffusion using monomethylether PEG 2000 as precipitant. The native MMCE crystals are orthorhombic, with unit-cell parameters a = 56.0, b = 114.0, c = 156.0 Å, and the SeMet-MMCE crystals are monoclinic, with unit-cell parameters a = 43.6, b = 78.6, c = 89.4 Å, β = 92.0°; both diffract to better than 2.8 Å resolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444901002050
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  • 6
    Electronic Resource
    Electronic Resource
    X-ray photoemission and absorption spectroscopy of supported nanoscale iron clusters (1999)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 86 (1999), S. 2651-2654 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Fe particles with sizes in the range 1–5 nm, formed by a gas-aggregation method and deposited onto graphite and C60 supports, were studied by x-ray photoemission spectroscopy, x-ray absorption spectroscopy, and magnetic linear dichroism. Clusters deposited onto a C60 coated graphite substrate become embedded within the fullerene film, and have an increased resistance to oxidation compared to exposed clusters supported on a graphite surface. No evidence for hybridization between the electronic states of Fe and C60 is seen. The magnetic dichroism signal of the exposed clusters increases sharply with the film thickness because of the increased cluster interactions. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.371105
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  • 7
    Electronic Resource
    Electronic Resource
    Particle transport in DIII-D discharges with internal regions of enhanced confinement and counter injected neutral beams (2001)
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 8 (2001), S. 1565-1572 
    Details
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: An analysis of experimentally measured particle transport in tokamak plasmas with negative central magnetic shear is presented. The analysis is presented in terms of a simple model for turbulent transport which allows the separation of diagonal and off diagonal terms and allows the direct comparison of particle and energy transport. Comparing the measured fluxes to the fluxes predicted by a simple quasi analytical model which specifies a relation between the diagonal and off diagonal terms allows an understanding of the reason for the difference between energy and particle fluxes. In the center of discharges with a region of enhanced confinement (or internal transport barrier), the ion thermal diffusivity becomes small and comparable to neoclassical values and the particle diffusivity also becomes small and approaches the neoclassical values. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1358311
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  • 8
    Electronic Resource
    Electronic Resource
    Decay of the daughter electromagnetic wave driven by stimulated Raman scattering into two Langmuir waves (2000)
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 7 (2000), S. 2759-2762 
    Details
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Much attention has been focused on the saturation of stimulated Raman scattering by the decay of its daughter waves. This research has almost exclusively dealt with the decay of the daughter Langmuir wave into secondary Langmuir waves and ion acoustic waves. In this Letter, the decay of the Raman driven electromagnetic wave into two Langmuir waves, two-plasmon decay, is discussed for the first time. It is shown that based on the stimulated Raman scattering reflectivity levels measured in experiments, this instability should be present in current laser–plasma experiments. In addition, experimental signatures of this process are discussed. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.874174
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  • 9
    Electronic Resource
    Electronic Resource
    Experimental constraints on transport from dimensionless parameter scaling studies : The 39th annual meeting of division of plasma physics of APS (1998)
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 5 (1998), S. 1695-1702 
    Details
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The scalings of heat transport with safety factor (q), normalized collisionality (ν), plasma beta (β), and relative gyroradius (ρ*) have been measured on the DIII-D tokamak [Fusion Technol. 8, 441 (1985)]. The measured ρ*, β and ν scalings of heat transport indicate that E×B transport from drift wave turbulence is a plausible basis for anomalous transport. For high confinement (H) mode plasmas where the safety factor was varied at fixed magnetic shear, the effective (or one-fluid) thermal diffusivity was found to scale like χeff∝q2.3±0.64 , with the ion and electron fluids having the same q scaling to within the experimental errors except near the plasma edge. The scaling of the thermal confinement time with safety factor was in good agreement with this local transport dependence, τth∝q−2.42±0.31 ; however, when the magnetic shear was allowed to vary to keep q0 fixed during the (edge) safety factor scan, a weaker global dependence was observed, τth∝q95−1.43±0.23. This weaker dependence was mainly due to the change in the local value of q between the two types of scans. The combined ρ*, β , ν and q scalings of heat transport for H-mode plasmas on DIII-D reproduce the empirical confinement scaling using physical (dimensional) parameters with the exception of weaker power degradation. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.872838
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  • 10
    Electronic Resource
    Electronic Resource
    The asymptotic motion of an accelerating, thick layer of inviscid liquid (1998)
    [S.l.] : American Institute of Physics (AIP)
    Physics of Fluids 10 (1998), S. 101-112 
    Details
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Most studies of a gravitationally unstable interface between a liquid and a gas by boundary integral techniques prescribe the motion of the liquid in the far field. The mean gas pressure at the interface is then irrelevant in its motion. On the other hand, when a pressure jump is applied to a liquid column in a vertical duct, its acceleration is determined by the pressure jump no matter how tall the column. Previous studies of accelerating liquid layers [G. R. Baker, R. L. McCrory, C. P. Verdon, and S. A. Orszag, "Rayleigh–Taylor instability of fluid layers," J. Fluid Mech. 178, 161 (1987)] show that the motion of the gravitationally unstable interface depends on the reciprocal of the mean layer thickness H. In this paper, we derive an asymptotic boundary integral method that captures the O(1/H) effects on the motion of the unstable interface with a correction that is exponentially small in H. The validity of the asymptotic approach is confirmed by comparison with numerical simulations of the liquid layer. The success of the approach relies on expansions of the kernels in the boundary integrals, indicating that the procedure for deriving the asymptotic equations is more general than just for vertical ducts or periodic geometry. In a subsequent paper, we use our approach to derive the equations for the formation of a bubble at a submerged orifice that is driven by an increase in gas pressure. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.869553
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