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1

Digitale Medien

Effects of ammonium ions on the depolarization-induced and direct activation of the contractile apparatus in mechanically skinned fast-twitch skeletal muscle fibres of the rat (1996)

Stephenson, Gabriela M. M. ; Stephenson, D. George

Springer

Journal of muscle research and cell motility 17 (1996), S. 611-616

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ISSN: 1573-2657

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Mechanically skinned fibre preparations from the extensor digitorum longus muscle of the rat were used to test whether a rise in myoplasmic [NH4 +] in the range 2–10 mm interferes with the mechanism of excitation-contraction coupling in fast-twitch mammalian muscle. Under our conditions (pH 7.10, Mg2+ 1 mm, temperature 23° C), [NH4 +] up to 10 mm had little effect on the Ca+-activated force and on the peak of the t-system depolarization-induced force response. However, the duration of the depolarization-induced force response was decreased significantly at [NH4 +] ≥2 mm. From these data we conclude that the intracellular accumulation of NH4 + is not likely to play a major role in fatigue. Nevertheless, the build up of NH4 + during fatigue, may have a significant inhibitory effect on the force output by decreasing the duration of the t-system depolarization-induced activation of the contractile apparatus.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00154055

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2

Digitale Medien

Microfluorometric analyses of glycogen in freshly dissected, single skeletal muscle fibres of the cane toad using a mechanically skinned fibre preparation (1998)

Nguyen, Long Thanh ; Stephenson, D. George ; Stephenson, Gabriela M. M.

Springer

Journal of muscle research and cell motility 19 (1998), S. 631-638

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ISSN: 1573-2657

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract The main objective of this study was to analyse glycogen in single muscle fibres, using a recently developed microfluorometric method which detects subpicomol amounts of NADPH, glucose and glycogen (as glucosyl units) (detection limit 0.16–0.17pmol in a 25nl sample) without fluorochrome amplification. The fibres were freshly dissected from the twitch region of the iliofibularis muscle of the cane toad (Bufo marinus), and were mechanically skinned under paraffin oil to gain access to the intracellular compartments. The results show that: (1) glycogen concentrations in toad skeletal muscle fibres range between 25.8 and 369mmol glucosyl units/litre fibre volume; (2) there is a large variation in glycogen content between individual fibres from the iliofibularis muscle of one animal; (3) there are seasonal differences in the glycogen content of toad single muscle fibres; (4) the total amount of glycogen in single muscle fibres of the toad does not decrease significantly when storing the tissue, under paraffin oil, at 20–25°C for up to 6h or at 4°C for up to 24h; and (5) 15–26% of fibre glycogen can be washed in an aqueous solution at pH 5–7, within 5min, while 74–85% of fibre glycogen remains associated with the washed skinned fibre, even after 40min exposure of the skinned fibre preparation to the aqueous environment. The retention of most glycogen in the fibre preparation after mechanical removal of the plasma membrane and extensive washing indicates that in toad skeletal muscle fibres the largest proportion of glycogen is tightly bound to intracellular structures. The results also show that the skinned muscle fibre preparation is well suited for microfluorometric glycogen determination, since low molecular weight non-glycogen contributors to the fluorescence signal can be removed from the myoplasmic space prior to the glycogen hydrolysis step.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1005377030193

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3

Unbekannt

A Direct Microfluorometric Method for Measuring Subpicomole Amounts of Nicotinamide Adenine Dinucleotide Phosphate, Glucose, and Glycogen (1998)

Nguyen, Long Thanh ; Stephenson, D.George ; Stephenson, Gabriela M.M.

Elsevier

In: Analytical Biochemistry. 1998; 259(2): 274-278. Published 1998 Jun 01. doi: 10.1006/abio.1998.2634.

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Publikationsdatum: 1998-06-01

Print ISSN: 0003-2697

Digitale ISSN: 1096-0309

Thema: Biologie , Chemie und Pharmazie

Publiziert von Elsevier

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4

Digitale Medien

A new locus for autosomal dominant retinitis pigmentosa on chromosome 7p (1993)

Inglehearn, Chris F. ; Carter, Simon A. ; Keen, T.Jeffery ; [weitere]

[s.l.] : Nature Publishing Group

Nature genetics 4 (1993), S. 51-53

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ISSN: 1546-1718

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Medizin

Notizen: [Auszug] Autosomal dominant retinitis pigmentosa (adRP) is known to result from mutations in two different retinal genes—rhodopsin and peripherin—while a third locus has been implicated by linkage data. However, families have been reported in which all three known loci have been excluded. We ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/ng0593-51

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5

Digitale Medien

Life-time of the Lithium Atomic Resonance States (1951)

STEPHENSON, G.

[s.l.] : Nature Publishing Group

Nature 167 (1951), S. 156-156

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] The Ladenburg absorption formula3 relating the integral of the absorption coefficient over the whole line to the Einstein transition probability coefficient Anm may be conveniently put in the form loge(I0/IV)dl(1)pNƒe2ll02/mc2 (1) where N is the number of normal atoms of absorbing vapour per ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/167156a0

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6

Digitale Medien

Possible Role of Proteolytic Enzymes in Protein Synthesis (1953)

LOFTFIELD, ROBERT B. ; GROVER, JOHN W. ; STEPHENSON, MARY L.

[s.l.] : Nature Publishing Group

Nature 171 (1953), S. 1024-1025

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] As a test of this hypothesis, Hendler and Green-berg5 have recently studied the metabolism of labelled y-glutamyl-glycine; but they found that this particular compound is not significantly involved in any of several living systems that incorporate amino-acids into proteins. The evidence presented ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/1711024a0

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7

Digitale Medien

Experimental Determination of the Life-times of Atomic Resonance States (1951)

STEPHENSON, G.

[s.l.] : Nature Publishing Group

Nature 167 (1951), S. 112-112

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] Further work is progressing with the view of adapting the method to molecular transitions, and the results will be reported at a later date. Atom Transition Wave-length Life-time (sec.) Na 32S-32P 5896, 5890 A. 1.61 0.06 x 10-8 K 42S-42P 7699, 7665 2.71 0.09 x 10-8 Rb 52S-52P 7947, 7800 2.81 ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/167112a0

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8

Digitale Medien

Use of Katharometers in Gas Chromatography (1959)

EDEN, MURRAY ; KARMEN, ARTHUR ; STEPHENSON, JOHN L.

[s.l.] : Nature Publishing Group

Nature 183 (1959), S. 1322-1322

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] His equation, dt/dx = a(k &2), states that the sensitivity of the detector, dt[dx, is proportional to the difference between the thermal conductivity of the carrier gas, kl9 and the vapour, k2, and inversely proportional to the square of the thermal conductivity of the carrier gas. As Ray ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/1831322a0

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9

Digitale Medien

Identity of ‘Nudic Acid B’ and ‘Diatretyne II’ (1957)

HEATLEY, N. G. ; STEPHENSON, J. S.

[s.l.] : Nature Publishing Group

Nature 179 (1957), S. 1078-1078

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] A sample of the original 'nudie acid B' (10 mgm.) was used for establishing the identity. This had been stored in darkness for ten years without any special precautions against decomposition and had gone black due to surface polymerization. However, when the sample (10 mgm.) was esterified with 3 ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/1791078a0

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10

Digitale Medien

Presence and Absence of the Pre-Natal Check in Lambs' Birthcoats (1954)

DRY, F. W. ; STEPHENSON, S. K.

[s.l.] : Nature Publishing Group

Nature 173 (1954), S. 878-879

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] Sickle-fibres constitute the most easily perceived evidence of the pre-natal check3-5. Of ten N/ + Aggrade lambs studied in a number of body regions, all had sickle-fibres on the shoulder patch and shoulder, and they were present on several necks, withers, sides and o backs, but not on any ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/173878b0

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