Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
FEMS microbiology letters
99 (1992), S. 0
ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract The deuteromycete Aphanocladium album produced two endochitinases (EC 3.2.1.14) with apparent isoelectric points of 7.1 and 7.6 and seven exochitinases (EC 3.2.1.30) with apparent isoelectric points ranging from 3.8 to 6.4 when grown on a colloidal chitin preparation. With crystalline chitin as carbon source, two endochitinases (pI 7.1 and 7.6) and only one exochitanase (pI 4.9) were detected. The exochitinase pI 4.9, which was produced with both substrates, has a relative molecular mass of 44 000. The different chitinases could be separated by chromatofocusing and their specific activities were determined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1992.tb05569.x
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