ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Three-dimensional gelatin-chondroitin 6 sulphate-hyanuronic acid biomatrix was used asthe scaffold to investigate the phenotypic and molecular expression in human keratinocytes (K) anddermal fibroblasts (FB) in three different culture conditions in vitro. The cells were cultured ineither monolayer (K or FB only) or coculture (K&FB) model. The deposition of basementmembrane proteins secreted by these two kinds of cells was quantitatively characterized byreal-time PCR. In the results, dermal fibroblasts were shown to synthesize and deposit laminin 5,type IV and type VII collagen, whereas keratinocytes produced integrin alpha 6 and beta 4 as wellas laminin 5 and collagen type IV, VII. Interestingly, the integrin beta 4 subunit was not expressedeither in keratinocytes or dermal fibroblasts monoculture but was seen in organotypic coculturemodel in the early culture period. Furthermore, we found that the expression of those markercompounds was reciprocally regulated when keratinocytes and dermal fibroblasts were culturedtogether. These results indicated that keratinocyes and dermal fibroblasts worked together toreconstruct dermal-epidermal basement membrane (BM) zone. In brief, our data provide the firsttime in directly quantifying the expression of BM proteins by using real-time PCR, and alsodemonstrate that BM proteins were regulated by cell-cell interaction
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.15-17.95.pdf
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