Publication Date:
2022-05-26
Description:
Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of AAAS for personal use, not for redistribution. The definitive version was published in Science 315 (2007): 247-250, doi:10.1126/science.1132913.
Description:
The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has
never been successfully cultivated outside its host. In the absence of cultivation data we
have taken a proteomic approach based on the metagenome sequence to study the
metabolism of this peculiar microorganism in detail. As one result, we found that three
major sulfide oxidation proteins constitute ~12% of the total cytosolic proteome,
highlighting the essential role of these enzymes for the symbiont’s energy metabolism.
Unexpectedly, the symbiont uses the reductive tricarboxylic acid (TCA) cycle in addition
to the previously identified Calvin cycle for CO2 fixation.
Description:
This work was supported by
the DFG, grant Schw595/3-1. Other funding sources were: NSF (OCE 04-52333) and NASA Astrobiology Institute (NNA04CC04A) for SMS, MH: postdoctoral
scholarship from WHOI, HF: Academic Senate (RF811S and RE518S).
Repository Name:
Woods Hole Open Access Server
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