Publication Date:
2005-06-11
Description:
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lindenbach, Brett D -- Evans, Matthew J -- Syder, Andrew J -- Wolk, Benno -- Tellinghuisen, Timothy L -- Liu, Christopher C -- Maruyama, Toshiaki -- Hynes, Richard O -- Burton, Dennis R -- McKeating, Jane A -- Rice, Charles M -- AI40034/AI/NIAID NIH HHS/ -- AI50798/AI/NIAID NIH HHS/ -- AI51820/AI/NIAID NIH HHS/ -- CA10702/CA/NCI NIH HHS/ -- CA57973/CA/NCI NIH HHS/ -- CA85883/CA/NCI NIH HHS/ -- DK70497/DK/NIDDK NIH HHS/ -- G0400802/Medical Research Council/United Kingdom -- New York, N.Y. -- Science. 2005 Jul 22;309(5734):623-6. Epub 2005 Jun 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15947137" target="_blank"〉PubMed〈/a〉
Keywords:
Antibodies, Monoclonal/immunology
;
Antibodies, Viral/immunology
;
Antigens, CD/metabolism
;
Antigens, CD81
;
Antiviral Agents/pharmacology
;
Cell Line, Tumor
;
Centrifugation, Density Gradient
;
Culture Media, Conditioned
;
Genome, Viral
;
Hepacivirus/genetics/immunology/*physiology
;
Humans
;
Interferon-alpha/pharmacology
;
Mutation
;
Neutralization Tests
;
RNA, Viral/biosynthesis
;
Replicon
;
Serial Passage
;
Transfection
;
Viral Envelope Proteins/analysis/biosynthesis
;
Viral Nonstructural Proteins/analysis/biosynthesis
;
Virion/physiology
;
*Virus Cultivation
;
*Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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