Publication Date:
2022-05-26
Description:
Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of IEE for personal use, not for redistribution. The definitive version was published in IEE Proceedings - Nanobiotechnology 152 (2005): 189-193, doi:10.1049/ip-nbt:20050040.
Description:
Membrane capacitance and membrane conductance values are reported for insulin secreting cells (primary β-cells and INS-1 insulinoma cells) determined using the methods of dielectrophoresis and electrorotation. The membrane capacitance value of 12.57 (± 1.46) mF/m2 obtained for β-cells, and the values 9.96 (± 1.89) mF/m2 to 10.65 (± 2.1) mF/m2 obtained for INS-1 cells, fall within the range expected for mammalian cells. The electrorotation results for the INS-1 cells lead to a value of 36 (± 22) S/m2 for the membrane conductance associated with ion channels, if values in the range 2nS to 3 nS are assumed for the membrane surface conductance. This membrane conductance value falls within the range reported for INS cells obtained using the whole-cell patch-clamp technique. However, the total ‘effective’ membrane conductance value of 601 (± 182) S/m2 obtained for the INS-1 cells by dielectrophoresis is significantly larger (by a factor of around three-fold) than the values obtained by electrorotation. This could result from an increased membrane surface conductance, or increased passive conduction of ions through membrane pores, induced by the larger electric field stresses experienced by cells in the dielectrophoresis experiments.
Description:
This study was financed by a gift from the Denis Robinson Memorial Fund to RP and NIH grants NCRR RR001395 and DK06984 to PJSS.
Keywords:
Electrokinetic measurements
;
Membrane capacitance
;
Pancreatic beta-cells
;
Membrane conductance
;
Insulin secreting cells
;
Insulinoma cells
;
Dielectrophoresis
;
Electrorotation
;
Mammalian cells
;
Ion channels
;
Membrane surface conductance
;
Whole-cell patch-clamp technique
;
Passive conduction
;
Membrane pores
;
Electric field stresses
;
Dielectrophoresis experiments
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
233829 bytes
Format:
application/pdf
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