Publication Date:
2008-04-19
Description:
Sequencing DNA from several organisms has revealed that duplication and drift of existing genes have primarily moulded the contents of a given genome. Though the effect of knocking out or overexpressing a particular gene has been studied in many organisms, no study has systematically explored the effect of adding new links in a biological network. To explore network evolvability, we constructed 598 recombinations of promoters (including regulatory regions) with different transcription or sigma-factor genes in Escherichia coli, added over a wild-type genetic background. Here we show that approximately 95% of new networks are tolerated by the bacteria, that very few alter growth, and that expression level correlates with factor position in the wild-type network hierarchy. Most importantly, we find that certain networks consistently survive over the wild type under various selection pressures. Therefore new links in the network are rarely a barrier for evolution and can even confer a fitness advantage.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666274/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666274/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Isalan, Mark -- Lemerle, Caroline -- Michalodimitrakis, Konstantinos -- Horn, Carsten -- Beltrao, Pedro -- Raineri, Emanuele -- Garriga-Canut, Mireia -- Serrano, Luis -- 066543/Wellcome Trust/United Kingdom -- England -- Nature. 2008 Apr 17;452(7189):840-5. doi: 10.1038/nature06847.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), UPF, 08003 Barcelona, Spain. isalan@crg.es〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18421347" target="_blank"〉PubMed〈/a〉
Keywords:
Escherichia coli/*genetics/growth & development/*metabolism
;
Escherichia coli Proteins/genetics/metabolism
;
*Evolution, Molecular
;
Gene Expression Regulation, Bacterial/*genetics
;
Gene Regulatory Networks/*genetics
;
Genes, Bacterial/genetics
;
*Genetic Engineering
;
Heat-Shock Response
;
Oligonucleotide Array Sequence Analysis
;
Open Reading Frames/genetics
;
Promoter Regions, Genetic/genetics
;
*Selection, Genetic
;
Serial Passage
;
Sigma Factor/genetics/metabolism
;
Transcription Factors/genetics/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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