Publication Date:
2002-12-01
Description:
The molecular backgrounds of variants encountered in Afro-Caribbean black individuals and associated with the production of clinically significant antibodies against high-incidence antigens (anti-RH18, anti-RH34) and against Rhe epitopes were determined. We showed that RH:−18 phenotypes are produced by 3 distinct RHCEalleles: ceEK carrying 48G〉C (exon 1), 712A〉G, 787A〉G, 800T〉A (exon 5); ceBI carrying 48G〉C (exon 1), 712A〉G (exon 5), 818C〉T (exon 6), 1132C〉G (exon 8); and the already knownceAR allele carrying 48G〉C (exon 1), 712A〉G, 733C〉G, 787A〉G, 800T〉A (exon 5), and 916A〉G (exon 6). The RH:−34 phenotype is produced by the (C)ces haplotype described previously and composed of a hybrid D-CE(3-8)-D gene with 4 extra mutations next to a ces allele (733C〉G; exon 5) with an extra mutation in exon 7 (1006G〉T). Partial Rhe with risk of immunization against lacking epitopes can be produced by the new ces allele carrying an extra mutation in exon 3 (340C〉T) and by the ceMO allele described previously. A population of sickle cell disease patients was screened to estimate the incidence of these rare alleles, with the conclusion that a procedure is required to detect the associated phenotypes in black donors to ensure transfusion safety for patients. We also described a new variant [ces(748)] and variants carrying different altered alleles in nonimmunized patients and for whom the risk of immunization is discussed.
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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