Publication Date:
2008-12-02
Description:
In teleosts, proper balance and hearing depend on mechanical sensors in the inner ear. These sensors include actin-based microvilli and microtubule-based cilia that extend from the surface of sensory hair cells and attach to biomineralized 'ear stones' (or otoliths). Otolith number, size and placement are under strict developmental control, but the mechanisms that ensure otolith assembly atop specific cells of the sensory epithelium are unclear. Here we demonstrate that cilia motility is required for normal otolith assembly and localization. Using in vivo video microscopy, we show that motile tether cilia at opposite poles of the otic vesicle create fluid vortices that attract otolith precursor particles, thereby biasing an otherwise random distribution to direct localized otolith seeding on tether cilia. Independent knockdown of subunits for the dynein regulatory complex and outer-arm dynein disrupt cilia motility, leading to defective otolith biogenesis. These results demonstrate a requirement for the dynein regulatory complex in vertebrates and show that cilia-driven flow is a key epigenetic factor in controlling otolith biomineralization.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821763/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821763/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Colantonio, Jessica R -- Vermot, Julien -- Wu, David -- Langenbacher, Adam D -- Fraser, Scott -- Chen, Jau-Nian -- Hill, Kent L -- M07185/PHS HHS/ -- R01 AI052348/AI/NIAID NIH HHS/ -- R01 AI052348-06A2/AI/NIAID NIH HHS/ -- R01 HL081799/HL/NHLBI NIH HHS/ -- R01AI52348/AI/NIAID NIH HHS/ -- England -- Nature. 2009 Jan 8;457(7226):205-9. doi: 10.1038/nature07520. Epub 2008 Nov 30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19043402" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Cilia/metabolism/*physiology
;
Dyneins/chemistry/deficiency/genetics/*metabolism
;
Epigenesis, Genetic
;
Humans
;
Microscopy, Video
;
Microtubule Proteins/chemistry/deficiency/genetics/*metabolism
;
Molecular Sequence Data
;
*Movement
;
Multiprotein Complexes/deficiency/genetics/metabolism
;
Otolithic Membrane/*cytology/*embryology/metabolism
;
Zebrafish/embryology/genetics/*metabolism
;
Zebrafish Proteins/chemistry/deficiency/genetics/*metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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