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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 85 (1955), S. 35-44 
    ISSN: 1573-5060
    Keywords: cereals ; protoplast transformation ; tissue electroporation ; particle bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Many aspects of basic and applied problems in plant biology can be investigated by transformation techniques. In dicotyledonous species, the ability to generate transgenic plants provides the tools for an understanding of plant gene function and regulation as well as for the directed transfer of genes of agronomic interest. For many dicotyledonous plants Agrobacterium tumefaciens can be routinely used to introduce foreign DNA into their genome. However, cereals seem to be recalcitrant to Agrobacterium-mediated transformation. In cereals, many efforts have been made in recent years to establish reliable transformation techniques. Several transformation techniques have been developed but to date only three methods have been found to be suitable for obtaining transgenic cereals: transformation of totipotent protoplasts, particle bombardment of regenerable tissues and, more recently, tissue electroporation. The current state of transformation methods used for cereals will be reviewed.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2008-08-02
    Print ISSN: 0167-6369
    Electronic ISSN: 1573-2959
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Springer
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  • 3
    Publication Date: 2006-08-01
    Print ISSN: 0236-5731
    Electronic ISSN: 1588-2780
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Published by Springer
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  • 4
    Publication Date: 2009-05-14
    Description: We investigate microseismic activity at the convergent plate boundary of the Hellenic subduction zone on- and offshore south-eastern Crete with unprecedented precision using recordings from an amphibian seismic network. The network configuration consisted of up to eight ocean bottom seismometers as well as five temporary short-period and six permanent broadband stations on Crete and surrounding islands. More than 2,500 local and regional events with magnitudes up to ML = 4.5 were recorded during the time period July 2003-June 2004. The magnitude of completeness varies between 1.5 on Crete and adjacent areas and increases to 2.5 in the vicinity of the Strabo trench 100 km south of Crete. Tests with different localization schemes and velocity models showed that the best results were obtained from a probabilistic earthquake localization using a 1-D velocity model and corresponding station corrections obtained by simultaneous inversion. Most of the seismic activity is located offshore of central and eastern Crete and interpreted to be associated with the intracrustal graben system (Ptolemy and Pliny trenches). Furthermore, a significant portion of events represents interplate seismicity along the NNE-ward dipping plate interface. The concentration of seismicity along the Ptolemy and Pliny trenches extends from shallow depths down to the plate interface and indicates active movement. We propose that both trenches form transtensional structures within the Aegean plate. The Aegean continental crust between these two trenches is interpreted as a forearc sliver as it exhibits only low microseismic activity during the observation period and little or no internal deformation. Interplate seismicity between the Aegean and African plates forms a 100-km wide zone along dip from the Strabo trench in the south to the southern shore-line of Crete in the north. The seismicity at the plate contact is randomly distributed and no indications for locked zones were observed. The plate contact below and north of Crete shows no microseismic activity and seems to be decoupled. The crustal seismicity of the Aegean plate in this area is generally confined to the upper 20 km in agreement with the idea of a ductile deformation of the lower crust caused by a rapid return flow of metamorphic rocks that spread out below the forearc. In the region of the Messara half-graben at the south coast of central Crete, a southward dipping seismogenic structure is found that coalesces with the seismicity of the Ptolemy trench at a depth of about 20 km. The accretionary prism south of Crete indicated by the Mediterranean Ridge showed no seismic activity during the observation period and seems to be deforming aseismically. © 2009 Springer Science+Business Media B.V.
    Print ISSN: 1383-4649
    Electronic ISSN: 1573-157X
    Topics: Geosciences , Physics
    Published by Springer
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