Publication Date:
2005-03-12
Description:
Antigen-presenting cells (APCs) internalize antigens and present antigen-derived peptides to T cells. Although APCs have been thought to exhibit a well-developed capacity for lysosomal proteolysis, here we found that they can exhibit two distinct strategies upon antigen encounter. Whereas macrophages contained high levels of lysosomal proteases and rapidly degraded internalized proteins, dendritic cells (DCs) and B lymphocytes were protease-poor, resulting in a limited capacity for lysosomal degradation. Consistent with these findings, DCs in vivo degraded internalized antigens slowly and thus retained antigen in lymphoid organs for extended periods. Limited lysosomal proteolysis also favored antigen presentation. These results help explain why DCs are able to efficiently accumulate, process, and disseminate antigens and microbes systemically for purposes of tolerance and immunity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Delamarre, Lelia -- Pack, Margit -- Chang, Henry -- Mellman, Ira -- Trombetta, E Sergio -- R37-AI34098/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2005 Mar 11;307(5715):1630-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15761154" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Antigen Presentation
;
Antigen-Presenting Cells/*enzymology/*immunology/metabolism
;
Antigens/*metabolism
;
B-Lymphocytes/enzymology/immunology/metabolism
;
Cells, Cultured
;
Dendritic Cells/*enzymology/immunology/metabolism
;
Endocytosis
;
Green Fluorescent Proteins/immunology/metabolism
;
Histocompatibility Antigens Class II/immunology
;
Horseradish Peroxidase/immunology/metabolism
;
Lymphoid Tissue/cytology/enzymology/immunology
;
Lysosome-Associated Membrane Glycoproteins
;
Lysosomes/*enzymology/ultrastructure
;
Macrophages/enzymology/immunology/metabolism
;
Membrane Glycoproteins/metabolism
;
Mice
;
Mice, Inbred C3H
;
Peptide Hydrolases/*metabolism
;
Ribonuclease, Pancreatic/immunology/metabolism
;
Ribonucleases/immunology/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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