Publication Date:
2005-05-28
Description:
The protein complement of cellular membranes is notoriously resistant to standard proteomic analysis and structural studies. As a result, membrane proteomes remain ill-defined. Here, we report a global topology analysis of the Escherichia coli inner membrane proteome. Using C-terminal tagging with the alkaline phosphatase and green fluorescent protein, we established the periplasmic or cytoplasmic locations of the C termini for 601 inner membrane proteins. By constraining a topology prediction algorithm with this data, we derived high-quality topology models for the 601 proteins, providing a firm foundation for future functional studies of this and other membrane proteomes. We also estimated the overexpression potential for 397 green fluorescent protein fusions; the results suggest that a large fraction of all inner membrane proteins can be produced in sufficient quantities for biochemical and structural work.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Daley, Daniel O -- Rapp, Mikaela -- Granseth, Erik -- Melen, Karin -- Drew, David -- von Heijne, Gunnar -- New York, N.Y. -- Science. 2005 May 27;308(5726):1321-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15919996" target="_blank"〉PubMed〈/a〉
Keywords:
Alkaline Phosphatase/analysis/genetics
;
Cell Membrane/*chemistry
;
Cloning, Molecular
;
Computational Biology
;
Cytoplasm/chemistry
;
Escherichia coli/*chemistry/genetics/ultrastructure
;
Escherichia coli Proteins/*analysis/chemistry/genetics/physiology
;
Gene Duplication
;
Genes, Bacterial
;
Green Fluorescent Proteins/analysis/genetics
;
Membrane Proteins/*analysis/chemistry/genetics/physiology
;
Periplasm/chemistry
;
Protein Structure, Secondary
;
*Proteome
;
Recombinant Fusion Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
