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  • Eichzusatzmethode  (1)
  • TEM  (1)
  • 2005-2009
  • 1980-1984  (2)
  • 1970-1974
  • 1950-1954
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  • 2005-2009
  • 1980-1984  (2)
  • 1970-1974
  • 1950-1954
Year
  • 1
    ISSN: 1432-072X
    Keywords: Schizosaccharomyces pombe ; Cell wall ; Membrane ; Acid phosphatase ; Freeze preparations ; SEM ; TEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to study the ultrastructure of the cell surface and plasma membrane of Schizosaccharomyces pombe as a function of growth conditions we investigated exponential and stationary phase cells grown in rich and minimal medium. Electron microscopic preparation techniques based on rapid cryofixation (without cryoprotectants) were used. The intramembraneous aspects of the plasma membrane were described by freeze fracturing. For the first time the dynamic surface structures could be directly analyzed by freeze drying in the scanning electron microscope and in thin section of freeze substituted samples. This preparation techniques reveal hair-like structures on the surface of yeast cells. The hairs of cells grown in the rich medium are longer than those grown in the minimal medium. A mutant defective in the structure of a cell surface galactomannoprotein (acid phosphatase) reveals (under conditions of maximal acid phosphatase expression) a cell surface structure that differs from the wild type. It is likely that the hairs represent the peripheral galactomannan layer or part of it. On the membrane fracture faces the number, shape, distribution and state of aggregation of the intramembraneous particles are different between membranes of growing and non-growing cells and between cells grown under different physiological conditions. In the minimal medium corresponding periodical structures on the plasmic and exoplasmic fracture faces were observed, which clearly differ between exponential and stationary phase cells. The number, length and depth of plasma membrane invaginations increase as the cells go from the exponential phase to the stationary phase. Short and flattened invaginations are filled with thin periodic structures.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 307 (1981), S. 109-114 
    ISSN: 1618-2650
    Keywords: Eichzusatzmethode ; stark gekrümmte Eichkurven ; Densitometrie ; FORTRAN-Programm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An iterative procedure has been applied to standard addition in order to overcome limitations so far involved with the standard addition technique. Using a FORTRAN-program even strongly nonlinear calibration functions could be evaluated. Moreover, in contrast to linear standard addition techniques remaining background absorption does hardly influence the result. Up to now this program has just been applied to densitometric data, but scientists are encouraged to use it whenever a standard addition technique is to be applied and calibration curves are not linear or background noise is observed.
    Notes: Zusammenfassung Der Einsatzbereich der Eichzusatzmethode konnte durch Anwendung eines iterativen Rechenverfahrens bedeutend vergrößert werden. Mit Hilfe eines FORTRAN-Programms wurde es möglich, stark gekrümmte Eichfunktionen der Eichzusatzmethode zugänglich zu machen. Darüber hinaus zeigt sich, daß eine Verschiebung der Eichkurve durch unspezifische Untergrundabsorption kaum Einfluß auf das analytische Ergebnis hat. Bislang wurde das Programm nur zur Auswertung densitometrisch gewonnener Daten benutzt. Dennoch möchten wir es auch für andere Analysenverfahren empfehlen, wenn eine Eichzusatzmethode bei nicht linearen Eichkurven eingesetzt werden soll. Bei Grundrauschen kann das Programm ebenfalls vorteilhaft eingesetzt werden.
    Type of Medium: Electronic Resource
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