Publication Date:
1988-12-02
Description:
In whole cell extracts of Saccharomyces cerevisiae, incubation of precursor mRNA transcripts encoding the sequences essential in vivo for forming the 3' end of the iso-1-cytochrome c mRNA (CYC1) revealed an endonuclease activity with the characteristics required for producing the mature mRNA 3' end. The observed cleavage in vitro is (i) accurate, occurring at or near the polyadenylation site of CYC1 RNA, (ii) 30 to 50 percent efficient, (iii) adenosine triphosphate dependent, (iv) specific for the 3' ends of at least two yeast pre-mRNA's, and (v) absent with related pre-mRNA's carrying mutations that abolish correct 3' end formation in vivo. In addition, a second activity in the extract polyadenylates the product under appropriate conditions. Thus, the mature 3' ends of yeast mRNA's may be generated by endonucleolytic cleavage and polyadenylation rather than by transcription termination.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Butler, J S -- Platt, T -- 5-RO1-GM35658/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1988 Dec 2;242(4883):1270-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Rochester Medical Center, NY 14642.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2848317" target="_blank"〉PubMed〈/a〉
Keywords:
Cytochrome c Group/*genetics
;
*Cytochromes c
;
Endoribonucleases/metabolism
;
In Vitro Techniques
;
Nucleotides/metabolism
;
Poly A/*genetics
;
*RNA Processing, Post-Transcriptional
;
RNA, Messenger/*genetics
;
Saccharomyces cerevisiae/*genetics
;
*Saccharomyces cerevisiae Proteins
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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