ISSN:
0887-6134
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Pentafluorobenzyl hydroxylamine has been used as a derivatization reagent in the analysis of corticosteroids by gas chromatography/negative ion chemical ionization (GC/NICI) mass spectrometry. The resulting pentafluorobenzyloxime-trimethylsilyl (PFBO/TMS) derivatives were generally formed in moderate yield but, despite this, the use of these derivatives resulted in a ∼ 10-fold improvement in the capability of identification of corticosteroids by GC/NICI mass spectrometry in comparison with the methoxime/TMS derivatives previously employed. The NICI mass spectra of PFBO/TMS derivatives were simple, with most of the ion current being carried by the [M - C6F6CH2]-. or [M - PFB]-. ion and by a reagent-specific peak at m/z 196. The derivatives have been employed in the quantification of ∼400 pg amounts of endogenous hydrocortisone present in samples of human aqueous humour and are readily applicable to the determination of low-picogram amounts of these compounds in biological media.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200180904
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