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The hyphae ofUromyces appendiculatus within the leaf tissue after high pressure freezing and freeze substitution (1988)

Welter, K. ; Müller, M. ; Mendgen, K.

Springer

Protoplasma 147 (1988), S. 91-99

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ISSN: 1615-6102

Keywords: Uromyces appendiculatus ; High pressure freezing ; Freeze substitution ; Cryoprotection ; Fine structure preservation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The fine structure of the intercellular dikaryotic hyphae of the biotrophic fungusUromyces appendiculatus was studied. High pressure freezing and freeze substitution were used to achieve a closer approximation of the native state than with conventional fixation and dehydration techniques. In addition to organelles previously described in rust fungi, heavily decorated multivesicular bodies (star bodies) were found close to the nuclei. Two types of tubular-vesicular complexes were distributed randomly within the cytoplasm of the hyphae. Furthermore, a more or less pronounced brush-like fibrillar layer on the hyphal walls was detected. The possibility that the latter two structures are correlated with the biotrophic phase of this fungus is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01403336

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Preparation and evaluation of OV-240-OH coated glass capillary columns for isomer specific determination of PCDDs and PCDFs (1987)

Schmid, P. ; Müller, M. D.

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 10 (1987), S. 548-552

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ISSN: 0935-6304

Keywords: Glass capillary gas chromatography ; Immobilization ; OV-240-OH cyanopropylpolysiloxane ; Isomer specificity ; Polychlorinated dibenzo-p-dioxins ; Polychlorinated dibenzofurans ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Glass capillaries were leached, dehydrated, persilylated with 1, 3-bis (3-cyanopropyl) tetramethyldisiloxane, and coated with OV-240-OH. After crosslinking and binding the phase to the glass surface the columns showed high separation efficiency, high temperature stability, and inertness comparable to persilylated apolar columns. Column performance is shown to be superior to liquid phase cyanopropyl columns such as SP 2330. The excellent separation capabilities together with the selectivity of the phase makes OV-240-OH coated columns a valuable tool for the determination of toxic isomers in complex mixtures of polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs). The order of elution of individual TCDD isomers was found to be similar to that described for SP 2330 or Silar 10c. The detection of PCDDs and PCDFs in a fly ash extract further illustrates the utility of OV-240-OH coated columns. The high temperature limit of these columns opens the way for the analysis of high boiling compounds such as mixed brominated/chlorinated dibenzo-p-dioxins and dibenzofurans.

Additional Material: 5 Ill.