ALBERT

Description: White clover (Trifolium repens L.) and red clover (T. pratense L.) are the most important legumes of temperate pastures. The former is used largely in systems based around sheep or cattle grazing and is grown together with a companion grass. Breeding aims to optimize the white clover contribution to the sward. This means that yield per se is not the aim but rather to take full advantage of the benefits of white clover; in particular, nitrogen fixation, high protein content, digestibility, mineral content and high intake. The objective is an agronomically and, as far as possible, nutritionally balanced sward, thus persistence of white clover and yield stability over a number of years are key goals. A considerable focus of germplasm improvement has therefore been overcoming biotic and abiotic stresses to clover performance. The former include not only pests and diseases but also the impact of the ruminant animal and the competitive interaction with the companion grass, while abiotic stress could be loosely defined as ‘winter hardiness’ and ‘summer survival’ depending on the site. In recent years the focus of many breeding efforts has shifted to give more consideration to the effects of variation within white clover germplasm on animal performance and the environment. Beneficial effects on productivity have been known for many years, but recent studies of the impact of forage diets on meat and milk quality have opened up new opportunities for improvement. Diffuse pollution of nitrogen and phosphorus from agricultural sources is high on the environmental protection agenda of many governments. Breeding efforts are now being made to reduce the contribution of clovers to both direct (leaching) and indirect (through animal returns) pollution. In particular, recent insights into mechanisms affecting protein breakdown in the rumen and silo offer new prospects for breeding interventions to reduce environmental impacts.Molecular marker methods are being developed in white clover and the transfer and use of resources and information accumulating in the model legumes Medicago truncatula and Lotus japonicus is likely to be a major route by which the power of genomic approaches is translated into forage legume improvement. Hybrids of white clover and related species have been developed to introgress key traits; namely, drought tolerance, grazing tolerance of large leaf types and enhanced seed yield, for which only limited genetic variation is present within the white clover gene pool.Red clover is less persistent than white clover, is typically cut three or more times in a season and is used to make silage for winter feed. Although it is often grown with a companion grass, monocultures are common and yield per se as well as persistency and pest and disease resistance are major breeding aims. Fewer agronomic studies and less germplasm improvement have been carried out in this species and molecular studies are not as well advanced although, as with white clover, future developments are likely to benefit greatly from a close relationship to model legumes. Red clover brings considerable benefits in terms of animal production and meat and milk quality. These aspects, alongside approaches to reduce nitrogenous pollution from the silo, represent considerable opportunities for variety development.

Description: SUMMARYThe growth and seed production potential of white clover (Trifolium repens L.) was studied in field plots in 2 years, with and without a companion grass (Lolium perenne L.). Three rates of grass suppressant, fluazifop-butyl (Fusilade), applied in early June, were compared on plots sown with the companion grass. Two clover varieties, Aberystwyth SI84 and Olwen, were compared.Sowing a companion grass reduced the amount of clover in the sward in both the first and second harvest years, but did not affect the vigour of individual clover growing points or the number of clover inflorescences when the crop was ready to be harvested for seed.Fluazifop-butyl, at the highest rate used (125g/ha), reduced the rates of leaf emergence and extension, and the numbers of reproductive tillers, in Lolium, without reducing the rate of leaf emergence in clover, although the weight per clover leaf was somewhat reduced. In the first harvest year, the numbers of SI84 inflorescences in June and July were increased by fluazifop-butyl, but the effect had worn off by harvest (24 August). Seed yield potential was not increased by fluazifop-butyl at the rates used.Olwen produced heavier leaves, but fewer leaves and fewer growing points/0·1 m2 than SI84. In the first harvest year, Olwen had fewer and smaller inflorescences than S184.It proved feasible to measure the weight of new leaf produced per week and the nett additions to stolon and inflorescence dry matter (DM). The estimate of nett addition to stolon DM was increased by increasing the number of visible internodes included in the calculation, up to the maximum number recorded (nine). The rate of leaf emergence was slower on young branches than on main axes.