ISSN:
1432-0983
Keywords:
Yeast
;
Yarrowia lipolytica
;
Protein secretion
;
Rab protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Small GTP-binding proteins of the Rab family are involved in the vesicular traffic inside eukaryotic cells. A gene library from the yeast Yarrowia lipolytica was screened with an oligonucleotide deduced from a highly conserved sequence in the Rab family. Four different genes were isolated. One of them, RYL1, was shown to be essential for cell viability. RYL1p displayed a high similarity with and tight phylogenetic relationships to SEC4p. When placed under the control of the GAL10 promoter, RYL1 was able to specifically relieve the thermosensitivity of a sec4–8 mutant of Saccharomyces cerevisiae. Therefore, it is proposed that RYL1 is a functional homologue of the S. cerevisiae SEC4 gene and is involved in the fusion of secretory vesicles with the plasma membrane in the general protein secretion pathway.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00313425
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