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All Library Books, journals and Electronic Records Telegrafenberg

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  • 1995-1999  (3)
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  • 1
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Interspecific hybridizations were performed between pelargonium × hortorum (subgenus Ciconium) and a yellow-flowered species. Pelargonium qitinquelobatum(subgenus Eumurpha). Hybrids, using embryo rescue, were obtained only when P. quinquelobatum was used as the female parent. A polymerase chain reaction (PCR) cloned Pelargonium chs gene fragment was used as probe to characterise hybrids hy restriction fragment length polymorphism (RFLP) analysis. Chromatographic flavonoid analysis showed that hybrids could synthesize both flavonol glycosides and anthocyanin in the petals which were pale yellow to pale pink from the proximal to the distal part.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell reports 15 (1995), S. 63-67 
    ISSN: 1432-203X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary TransgenicPelargonium X hortorum have been producedvia Agrobacterium tumefaciens-mediated transformation. The regeneration protocol used provided a regeneration frequency approximately to 95 percent. Clumps of regenerants, from cotyledons and hypocotyls ofPelargonium X hortorum seedlings, were inoculated with the disarmed strain EHA101 ofAgrobacterium tumefaciens. This strain contains a binary vector carrying neomycin phosphotransferase II, hygromycin B phosphotransferase and ß-glucuronidase genes. Selection on the regeneration medium supplemented with hygromycin allowed production of transgenic plants in up to 20% of the inoculated explants. The insertion of foreign DNA was demonstrated by Southern and polymerase chain reaction analysis: these experiments indicated that the inserted T-DNA is not full length for most of the plants. All RO transgenic plants exhibited a normal phenotype and are fertile.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell, tissue and organ culture 49 (1997), S. 209-212 
    ISSN: 1573-5044
    Schlagwort(e): Pelargonium peltatum ; shoot organogenesis ; somatic embryogenesis ; thidiazuron
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Regeneration from petioles and leaf blades was studied for seven genotypes of Pelargonium peltatum. Multiple adventitious shoots were produced using wide range of thidiazuron concentrations. Somatic embryos were produced from callus-derived cell suspensions from 3 genotypes, with a combination of 0.45 µM thidiazuron and 20 µM α-naphthaleneacetic acid in liquid medium. Regenerants were rooted and transferred to soil where they showed a normal phenotype.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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