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  • Keywords: esterase; Bacillus circulans; bacterial growth; enzyme production  (1)
  • Zymomonas mobilis  (1)
  • 2005-2009
  • 1995-1999  (2)
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Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Effect of culture conditions on growth and esterase production by the moderate thermophile Bacillus circulans MAS2 (1999)
    Springer
    Journal of industrial microbiology and biotechnology 23 (1999), S. 188-193 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: esterase; Bacillus circulans; bacterial growth; enzyme production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth and esterase production (activity on p-nitrophenyl caprylate) by the newly isolated Bacillus circulans MAS2 bacterial strain were studied. The growth rate at 50°C was high (0.9 h-1) on LB medium with glucose added. Esterase production followed growth with the majority of activity being intracellular during exponential growth phase. During stationary phase, the esterase activity was released in the culture medium. The strain was able to grow at 35– 55°C with maximum growth rate at 50°C, showing a pattern typical of a moderate thermophile. Growth occurred at pH 6–9 with a maximum at 8, with a similar pattern for the esterase production. Addition of glucose, fructose, sucrose or sodium acetate greatly promoted both growth and esterase production while starch, inulin, tributyrin or glycerol showed no effect. Complex nitrogen sources such as tryptone or yeast extract increased growth and esterase production while mineral sources (ammonium chloride or sulfate), glycine or glutamate showed no effect. An increase of tryptone plus yeast extract and glucose concentrations stimulated growth and esterase production which reached 160 U L−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900717
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of the extracellular sucrase gene (sacC) of Zymomonas mobilis (1995)
    Springer
    Archives of microbiology 163 (1995), S. 195-204 
    Details
    ISSN: 1432-072X
    Keywords: Key words Extracellular sucrase ; Sucrase ; Invertase ; Zymomonas mobilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Zymomonas mobilis gene sacC that encodes the extracellular sucrase (protein B46) was cloned and expressed in Escherichia coli. The gene was found to be present downstream to the already described levansucrase gene sacB in the cloned chromosomal fragment of Z. mobilis. The expression product was different from SacB and exhibited sucrase but not levansucrase activity; therefore, SacC behaves like a true sucrase. Expression of sacC in E. coli JM109 and XL1 was very low; overexpression was observed in E. coli BL21 after induction of the T7 polymerase expression system with IPTG. Subcellular fractionation of the E. coli clone carrying plasmid pLSS2811 showed that more than 70% of the sucrase activity could be detected in the cytoplasmic fraction, suggesting that the enzyme was soluble and not secreted in E. coli. The nucleotide sequence analysis of sacC revealed an open reading frame 1239 bp long coding for a 413 amino acid protein with a molecular mass of 46 kDa. The first 30 deduced amino acids from this ORF were identical with those from the N-terminal sequence of the extracellular sucrase (protein B46) purified from Z. mobilis ZM4. No leader peptide sequence could be identified in the sacC gene. The amino acid sequence of SacC showed very little similarity to those of other known sucrases, but was very similar to the levansucrases of Z. mobilis (61.5%), Erwinia amylovora (40.2%) and Bacillus subtilis (25.6%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305353
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