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  • Cell electrophoresis  (1)
  • Glucose  (1)
  • Wiley-Blackwell  (2)
  • American Physical Society
  • 2005-2009
  • 1995-1999  (2)
Collection
Publisher
  • Wiley-Blackwell  (2)
  • American Physical Society
Years
  • 2005-2009
  • 1995-1999  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electroanalysis 7 (1995), S. 83-87 
    ISSN: 1040-0397
    Keywords: Flexible biosensor ; Gold-coated membrane ; Glucose ; Nontoxicity ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A flexible glucose biosensor was constructed by immobilizing glucose oxidase within a gold-coated, hydrophilic polytetrafluoroethylene membrane. All reagents and materials used were harmless and nontoxic. Enzyme immobilization was improved using a mesh mask during gold deposition, the grid pattern permitting better access into the membrane. The masked sensors had greatly improved output currents and linearity. The sensor was linear up to 662 mgL-1, the range covering the concentrations encountered in normal and diabetic human tear fluids. The current was proportional to the square of the electrode area, hence an electrode of known dimensions can be used without precalibration. Sterilization using 80% ethanol had a negligible effect on its response. The sensor is well suited to the detection of glucose or other analytes in body fluids, such as tears, airway mucus, sweat, and saliva.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0173-0835
    Keywords: Cell electrophoresis ; Erythrocytes ; Systemic lupus erythematosus ; Glycerol lysis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Using an automated cell electrophoresis system equipped with an image processor, we studied electrophoretic mobilites of erythrocytes of healthy donors and of patients suffering from systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), hypergammaglobulinemia and diabetes mellitus (DM). On average, erythrocytes from SLE patients showed mean electrophoretic mobilities (EPM) which were significantly lower (p 〈 0.005) than the EPM of red blood cells of normal donors. Evaluation of mean EPM and standard deviations revealed that three groups of SLE patients could be distinguished regarding the electrophoretic behavior of their erythrocytes. Some patients had red blood cells with normal EPM, others had erythrocytes with significantly reduced EPM, and a third group appeared to have both kinds of erythrocytes. In addition, erythrocytes of various SLE patients showed enhanced resistance to lysis by glycerol and their membranes contained less quantities of band 3 proteins.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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