ISSN:
1432-203X
Keywords:
Key wordsJuncus accuminatus
;
Callus regeneration
;
Medium pH
;
Phytoremediation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A reliable callus regeneration and shoot multiplication system for wetland monocot Juncus accuminatus has been established. Callus was induced from 6-day-old seedlings on Murashige and Skoog medium supplemented with 5 mg/l picloram. The callus differentiated into shoots upon transfer to 5 mg/l benzyladenine (BA)-supplemented medium. Effects of medium pH (3.8–7.8) and source of callus (grown in the dark or continuous light) on regeneration were determined. Both parameters significantly influenced regeneration. Regenerated shoots were multiplied by subculturing shoots onto 5 mg/l BA medium at 4-week intervals. The regenerated shoots were rooted on 0.1 mg/l naphthaleneacetic-acid-supplemented medium. The rooted plants were transferred to pots containing a commercial potting mix and established in the greenhouse. Plants covered with plastic grew faster and flowered earlier than uncovered plants. All plants set viable seeds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050460
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