ISSN:
1573-5087
Keywords:
barley
;
distribution
;
GC-MS
;
gibberellin
;
gibberellin-O-glucoside
;
reconversion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The distribution of GA20 and GA20-13-O-glucoside was examined in mature caryopses of Hordeum vulgare L. cv. Himalaya. It was shown by GC-MS that GA20-13-O-glucoside is localized mainly in the embryo-containing part of the grain. In order to study the reconversion of GA20-13-O-glucoside during early germination, the glucoside pool of cv. Himalaya was [17-D2]-labelled by feeding [17-D2]GA20. The metabolites of [17-D2]GA20-13-O-glucoside were quantified by GC-MS during germination on the basis of doubly labelled standards. As soon as 6 h after incubation an increase of [17-D2]GA20 could be observed, followed by an increase of [17-D2]GA1 after 12 h, showing that the enzymes of the late GA metabolism are present before de novo biosynthesis started.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006218530757
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