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  • Cybernetics, Artificial Intelligence and Robotics  (1)
  • Protease  (1)
  • 2005-2009
  • 1995-1999  (1)
  • 1985-1989  (1)
  • 1
    ISSN: 0173-0835
    Keywords: Two-dimensional zymography ; Photorhabdus luminescens ; Protease ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional zymography which combines two-dimensional electrophoresis with zymography was used to analyze proteases and other proteins produced by different phase variants of two strains of Photorhabdus luminescens. Both the primary and secondary phases of P. luminescens strains Hp and Hm secreted proteases. The protease in P. luminescens Hp has a molecular weight (Mr) of 57 000 and an isoelectric point (pI) of 4.4 whereas that in P. luminescens Hm has an Mr of 59 000 and pI of 4.9. Several putative protease degradation products were clearly visible in the zymograms from both bacterial strains. Two-dimensional zymography also showed that several secretory proteins were present only in particular phase variants and therefore could be used as specific markers. Unexpectedly, the two-dimensional zymography revealed that a nonsecretory protease with an Mr of 47 000 and a pI of 4.0 was present in the cell extracts of all phases of both P. luminescens Hp and Hm. The application of the two-dimensional zymography for the identification of other enzymes was also discussed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-08-14
    Description: Expert Systems which support knowledge representation by qualitative modeling techniques experience problems, when called upon to support integrated views embodying description and explanation, especially when other factors such as multiple causality, competing rule model resolution, and multiple uses of knowledge representation are included. A series of prototypes are being developed to demonstrate the feasibility of automating the process of systems engineering, design and configuration, and diagnosis and fault management. A study involves not only a generic knowledge representation; it must also support multiple views at varying levels of description and interaction between physical elements, systems, and subsystems. Moreover, it will involve models of description and explanation for each level. This multiple model feature requires the development of control methods between rule systems and heuristics on a meta-level for each expert system involved in an integrated and larger class of expert system. The broadest possible category of interacting expert systems is described along with a general methodology for the knowledge representation and control of mutually exclusive rule systems.
    Keywords: Cybernetics, Artificial Intelligence and Robotics
    Type: NASA, Marshall Space Flight Center, Second Conference on Artificial Intelligence for Space Applications; p 563-581
    Format: text
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