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  • Artikel  (186)
  • Springer  (138)
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  • Nature Publishing Group  (6)
  • American Meteorological Society (AMS)
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  • Artikel  (186)
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  • 1
    Digitale Medien
    Digitale Medien
    Glycosylation of glycoprotein 55 encoded by the anaemia-inducing strain of Friend spleen focus-forming virus (1994)
    Springer
    Glycoconjugate journal 11 (1994), S. 133-139 
    Details
    ISSN: 1573-4986
    Schlagwort(e): Friend spleen focus-forming virus ; glycoprotein ; oligosaccharide processing ; SFFV
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract Normal rat kidney cells, non-productively infected with the anaemia-inducing variant of Friend spleen focus-forming virus (F-SFFVA), were metabolically labelled with [2-3H]mannose. The primary translation product of the viral envelope gene (env), representing a glycoprotein with an apparent molecularM r of 55 000 (gp55), was isolated from cell lysates by immunoaffinity chromatography and purified by preparative SDS/PAGE. Radiolabelled oligosaccharides, released from tryptic glycopeptides by treatment with endo-β-N-acetylglucosaminidase H, were characterized chromatographically, by enzymic digestion and by acetolysis. The results revealed that F-SFFVA gp55 obtained from this source carried predominantly oligomannose type sugar chains with five to nine mannoses. As a characteristic feature, glycans with seven to nine mannoses contained, in part, an additional glucose residue. Although the amount of glucosylated species found was higher in F-SFFVA gp55 (about 25% of total endo-H-sensitive oligosaccharides) than in gp55 of the corresponding polycythaemia-inducing variant (F-SFFVP, 16.3%), the overall glycosylation pattern of the F-SFFVA env product closely resembled that of F-SFFVP gp55 [Strubeet al. (1988)J Biol Chem 263:3762–71]. Hence, our results demonstrate that the different intracellular processing and transport of the primary F-SFFVA env product cannot be attributed to aberrant trimming of its oligomannose type glycans.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00731153
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Host-cell-specific glycosylation of HIV-2 envelope glycoprotein (1997)
    Springer
    Glycoconjugate journal 14 (1997), S. 785-793 
    Details
    ISSN: 1573-4986
    Schlagwort(e): glycoprotein ; glycosylation ; gp120 ; HIV ; MALDI-TOF-MS
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract Neutral complex-type N-glycans of the envelope glycoprotein 120 of HIV-2, propagated in different host cells, display cell-type specific variations. In order to identify typical structural elements, glycans were analysed by gel filtration, by enzymic sequencing and, in part, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The characteristic substituents of di- tri- and tetraantennary carbohydrate units thus observed include N-acetyllactosamine repeats, bisecting N-acetylglucosamine and fucose linked to the chitobiose core as well as to N-acetyllactosamine antennae. Each glycoprotein preparation displayed a characteristic set of glycoforms. Abbreviations: endo H, endo-β-N-acetylglucosaminidase H; E-PHA, Phaseolus vulgaris agglutinin E4; GlcNAcOH, N-acetyl-glucosaminitol; gp120/HUT78(MOLT4/Mφ/PBL/U937), external envelope glycoprotein 120 of HIV-2, strain D194, propagated in HUT78 (MOLT4, Mφ, PBL, U937) cells; gu, glucose units; HPAEC, high-pH anion-exchange chromatography; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Mφ, human monocytes/macrophages; PBL, human peripheral blood lymphocytes; PNGase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018577619036
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Spectroscopic properties of dissolved humic substances - a reflection of land use history in a fen area (1999)
    Springer
    Biogeochemistry 47 (1999), S. 219-238 
    Details
    ISSN: 1573-515X
    Schlagwort(e): dissolved humic substances ; FTIR spectroscopy ; land use history ; peat ; synchronous fluorescence spectroscopy ; UV/VIS spectroscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Geologie und Paläontologie
    Notizen: Abstract The elemental composition and spectroscopic properties of dissolved fulvic acids isolated from different sampling media (topsoil, ground and surface water) of a natural fen area (high portion of organic soils) were examined to reveal the effects of land use history. These effects need to be known if dissolved humic substances are to be a major factor in identifying the impact of present and future changes in land use. Dissolved fulvic acids (topsoil, groundwater) from highly degraded peatlands (due to a long-term agricultural use) exhibit lower C/N ratios, higher absorption in the UV spectra, and higher absorption at 1,620 cm−1 in the FTIR spectra compared with fulvic acids from relatively intact peatlands. These properties illustrate that long-term agricultural use with high inputs results in increased aromatic structures and a further humification of dissolved fulvic acids due to very strong peat decomposition compared with relatively intact peatlands. Synchronous fluorescence spectra also indicate the higher level of aromatic structures within fulvic acids isolated from sites with long-term agricultural use (high peat decomposition) compared with a land use history resulting in a lower peat decomposition. The different sources of fulvic acids in surface water (precipitation, runoff, interflow, groundwater) are the main reason for these effects not being detected in fulvic acids isolated from surface water. Short-term changes in land use characterized by a transition from crop farming to an unimproved grassland were found not to affect the spectroscopic properties of dissolved fulvic acids. A humification index deduced from the synchronous fluorescence spectra is proposed. We have strong evidence that dissolved humic substances indicate changes in the environmental conditions (both anthropogenic and natural) of wetlands with a high proportion of organic soils.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006134214244
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Spectroscopic properties of dissolved humic substances — a reflection of land use history in a fen area (1999)
    Springer
    Biogeochemistry 47 (1999), S. 219-238 
    Details
    ISSN: 1573-515X
    Schlagwort(e): dissolved humic substances ; FTIR spectroscopy ; land use history ; peat ; synchronous fluorescence spectroscopy ; UV/VIS spectroscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Geologie und Paläontologie
    Notizen: Abstract The elemental composition and spectroscopic properties of dissolved fulvic acids isolated from different sampling media (topsoil, ground and surface water) of a natural fen area (high portion of organic soils) were examined to reveal the effects of land use history. These effects need to be known if dissolved humic substances are to be a major factor in identifying the impact of present and future changes in land use. Dissolved fulvic acids (topsoil, groundwater) from highly degraded peatlands (due to a long-term agricultural use) exhibit lower C/N ratios, higher absorption in the UV spectra, and higher absorption at 1,620 cm−1 in the FTIR spectra compared with fulvic acids from relatively intact peatlands. These properties illustrate that long-term agricultural use with high inputs results in increased aromatic structures and a further humification of dissolved fulvic acids due to very strong peat decomposition compared with relatively intact peatlands. Synchronous fluorescence spectra also indicate the higher level of aromatic structures within fulvic acids isolated from sites with long-term agricultural use (high peat decomposition) compared with a land use history resulting in a lower peat decomposition. The different sources of fulvic acids in surface water (precipitation, runoff, interflow, groundwater) are the main reason for these effects not being detected in fulvic acids isolated from surface water. Short-term changes in land use characterized by a transition from crop farming to an unimproved grassland were found not to affect the spectroscopic properties of dissolved fulvic acids. A humification index deduced from the synchronous fluorescence spectra is proposed. We have strong evidence that dissolved humic substances indicate changes in the environmental conditions (both anthropogenic and natural) of wetlands with a high proportion of organic soils.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00994924
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
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    Spectroscopic properties of dissolved humic substances ? a reflection of land use history in a fen area (1999)
    Springer
    Details
    Publikationsdatum: 1999-11-01
    Print ISSN: 0168-2563
    Digitale ISSN: 1573-515X
    Thema: Chemie und Pharmazie , Geologie und Paläontologie
    Publiziert von Springer
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    facet.materialart.
    Unbekannt
    Glycosylation of glycoprotein 55 encoded by the anaemia-inducing strain of Friend spleen focus-forming virus (1994)
    Springer
    Details
    Publikationsdatum: 1994-04-01
    Print ISSN: 0282-0080
    Digitale ISSN: 1573-4986
    Thema: Chemie und Pharmazie
    Publiziert von Springer
    Standort Signatur Erwartet Verfügbarkeit
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    AKTUELLE ARTIKEL
  • 7
    Digitale Medien
    Digitale Medien
    Two different areas within the primary motor cortex of man (1996)
    [s.l.] : Nature Publishing Group
    Nature 382 (1996), S. 805-807 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] We analysed sections of five human brains with quantitative cytoarchitectonic methods13. The border between somatosensory area 3a and the posterior part of area 4 was sharp. Large, slender pyramidal neurons in layer III characterized area 6, and the transition to the anterior part of area 4 was ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/382805a0
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    NMR structure of inactivation gates from mammalian voltage-dependent potassium channels (1997)
    [s.l.] : Nature Publishing Group
    Nature 385 (1997), S. 272-275 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The functional properties of inactivation domains of Kv channels have been analysed in studies combining electrophysiological measurements with site-direct mutagenesis. Both electrostatic and hydrophobic interactions were found to determine the affinity of the ball domain for its binding site in ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/385272a0
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Free Radical Reactions with Inorganic Covalent Hydrides: a Route to Polyfluoroalkyl Silicon Compounds (1956)
    [s.l.] : Nature Publishing Group
    Nature 178 (1956), S. 808-808 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Alkylsilyl radicals should be more reactive than trihalogenosilyl radicals, and a general study of their chemistry has been initiated. In particular, their reactions with olefins provide a useful new route for the synthesis of silicon compounds, and especially of polyfluoroalkyl silicon compounds. ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/178808a0
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Norbert Wiener and the Social Sciences (1994)
    Bingley : Emerald
    Kybernetes 23 (1994), S. 46-61 
    Details
    ISSN: 0368-492X
    Quelle: Emerald Fulltext Archive Database 1994-2005
    Thema: Informatik
    Notizen: Aims to analyse the influence of Norbert Wiener's ideas on the social sciences and on social systems, including society as a whole. Describes Wiener's own attitudes regarding the applicability of cybernetics to social systems and his vision on the development of modern society. Highlights sociologists and political scientists who were inspired by his ideas and deals with researchers who tried to apply his ideas to social systems. Concludes by evaluating to what extent specific ideas of Wiener have impacted on the social sciences.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1108/03684929410068334
    Standort Signatur Erwartet Verfügbarkeit
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