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  • Pythium  (2)
  • Biochemistry and Biotechnology  (1)
  • Photorhabdus luminescens  (1)
  • 2005-2009
  • 1995-1999  (2)
  • 1990-1994  (1)
  • 1975-1979
Collection
Keywords
Publisher
Years
  • 2005-2009
  • 1995-1999  (2)
  • 1990-1994  (1)
  • 1975-1979
Year
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of circular mitochondrial plasmids in three Pythium species (1991)
    Springer
    Current genetics 20 (1991), S. 91-97 
    Details
    ISSN: 1432-0983
    Keywords: Pythium ; Mitochondrial plasmid ; RNA transcript
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four circular plasmids, with a monomer size ranging from 3.2 to 4.94 kb, have been identified in isolates of P. aphanidermatum (two different plasmids), P. torulosum, and an unidentified echinulate isolate. The mitochondrial location has been confirmed for three of the plasmids. Each fungal isolate contained a single plasmid, present in both monomeric and oligomeric forms; plasmid monomers were present as open circles and as supercoiled forms. Restriction maps of the plasmids were dissimilar. Hybridization studies using cloned plasmids revealed no DNA sequence similarity among the different plasmids or between the plasmids and the nuclear or mitochondrial genome of the isolates from which they were recovered. Hybridization of labeled plasmid DNA to Northern transfers of mitochondrial RNA for two isolates indicate that what appears to be the predominant RNA transcript is unit length in size. For three isolates, the plasmid was retained following subculturing and was present in all asexual and sexual single-spore progeny evaluated. For one isolate of P. aphanidermatum the plasmid was unstable and was lost during subculturing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312771
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  • 2
    Electronic Resource
    Electronic Resource
    Linear mitochondrial genome organization in vivo in the genus Pythium (1995)
    Springer
    Current genetics 28 (1995), S. 225-234 
    Details
    ISSN: 1432-0983
    Keywords: Pythium ; Linear mitochondrial DNA ; Pulsed-field gel electrophoresis ; ATPase9 ; mtDNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pulsed-field gel electrophoresis (PFGE) of isolates of Pythium oligandrum with linear mitochondrial genomes revealed a distinct band in ethidium bromide-stained gels similar in size to values estimated by restriction mapping of mitochondrial DNA (mtDNA). Southern analysis confirmed that these bands were mtDNA and indicated that linear genomes were present in unit-length size as well as multimers. Isolates of this species with circular mtDNA restriction maps also had low levels of linear mono- and multimers. visualized by Southern analysis of PFGE gels. Examination of 17 additional species revealed similar results; three species had distinct linear mtDNA bands in ethidium bromide-stained gels while the remainder had linear mono- and multi-mers in lower amounts detected only by Southern analysis. Sequence analysis of an isolate of P. oligandrum with a primarily circular mitochondrial genomic map and a low amount of linear molecules revealed that the small unique region of the circular map (which corresponded to the terminal region of linear genomes) was flanked by palindromic intrastrand complementary sequences separated by a unique 194-bp sequence. Sequences with similarity to ATPase9 coding regions from other organisms were located adjacent to this region. Sequences with similarity to mitochondrial origins of replication and autonomously replicating sequences were also located in this region: their potential involvement in the generation of linear molecules is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309781
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of proteins from different phase variants of the entomopathogenic bacteria Photorhabdus luminescens by two-dimensional zymography (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 834-839 
    Details
    ISSN: 0173-0835
    Keywords: Two-dimensional zymography ; Photorhabdus luminescens ; Protease ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional zymography which combines two-dimensional electrophoresis with zymography was used to analyze proteases and other proteins produced by different phase variants of two strains of Photorhabdus luminescens. Both the primary and secondary phases of P. luminescens strains Hp and Hm secreted proteases. The protease in P. luminescens Hp has a molecular weight (Mr) of 57 000 and an isoelectric point (pI) of 4.4 whereas that in P. luminescens Hm has an Mr of 59 000 and pI of 4.9. Several putative protease degradation products were clearly visible in the zymograms from both bacterial strains. Two-dimensional zymography also showed that several secretory proteins were present only in particular phase variants and therefore could be used as specific markers. Unexpectedly, the two-dimensional zymography revealed that a nonsecretory protease with an Mr of 47 000 and a pI of 4.0 was present in the cell extracts of all phases of both P. luminescens Hp and Hm. The application of the two-dimensional zymography for the identification of other enzymes was also discussed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180530
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