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  • 1
    Electronic Resource
    Electronic Resource
    Effect of high altitude on sensitivity to the taste of phenylthiocarbamide (2000)
    Springer
    International journal of biometeorology 44 (2000), S. 20-23 
    Details
    ISSN: 1432-1254
    Keywords: Key words PTC taste sensitivity ; Taster ; Non-taster ; High altitude
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract  Sensitivity to the taste of phenylthiocarbamide (PTC) was studied using the Harris-Kalmus method in healthy human volunteers at sea level and then subsequently at an altitude of 3500 m over a period of 3 weeks, after which they were brought back to sea level. Blood sugar, insulin and blood cortisol levels were estimated weekly. The results indicated that, out of 51 subjects studied, 26 (55%) were PTC tasters at sea level. Eight of those unable to taste PTC at sea level tested as tasters at high altitude, and 2 of them reverted to being non-tasters on return to sea level. In the blood, an increase in cortisol and blood insulin levels was seen without any significant change in sugar levels. All the changes recorded at high altitude tended to return to basal values after re-induction to sea level. The study suggests that high-altitude hypoxia in some way, possibly involving changes in hormonal profile among other factors, causes an alteration in sensitivity to the taste of PTC, resulting in some of the individuals shifting to lower PTC sensitivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004840050134
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of hygromycin-resistant transformants of thermophilic fungus Thermomyces lanuginosus (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 303-306 
    Details
    ISSN: 1573-0972
    Keywords: Antagonistic metabolites ; characterization ; hygromycin-resistant ; transformants ; Thermomyces lanuginosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Hygromycin-resistant stable transformants of the thermophilic fungus, Thermomyces lanuginosus, were obtained by electroporation of germinating aleurospores with a plasmid pMP6, coding for hygromycin resistance. Southern hybridization analysis revealed that the gene is integrated into the chromosome. The hygromycin-resistant transformants were characterized for morphological changes, growth response towards the presence of antagonistic metabolites (hygromycin, 2-deoxy-D-glucose, cylcoheximide, benlate and acriflavine) on plates and enzyme production (amylases, pectinases and xylanase) in shake flask cultures. A hygromycin-resistant transformant hyg 33 was characterized as non-sporulating, 2-deoxy-D-glucose-resistant, acriflavine-sensitive and xylanase hypo-producer and is being used as parental strain for breeding strains through protoplast fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008934809245
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