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  • Articles  (251)
  • 2005-2009  (133)
  • 2000-2004  (112)
  • 1940-1944  (6)
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  • 1
    Electronic Resource
    Electronic Resource
    The Condensation of Isobutyraldehyde with Aliphatic Ketones (1944)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 66 (1944), S. 372-376 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja01231a017
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  • 2
    Electronic Resource
    Electronic Resource
    The gene sll0273 of the cyanobacterium Synechocystis sp. strain PCC6803 encodes a protein essential for growth at low Na+/K+ ratios (2000)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 23 (2000), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A mutant of Synechocystis sp. strain PCC6803 was obtained by random cartridge mutagenesis, which could not grow at low sodium concentrations. Genetic analyses revealed that partial deletion of the sll0273 gene, encoding a putative Na+/H+ exchanger, was responsible for this defect. Physiological characterization indicated that the sll0273 mutant exhibited an increased sensitivity towards K+, even at low concentrations, which was compensated for by enhanced concentrations of Na+. This enhanced Na+ demand could also be met by Li+. Furthermore, addition of monensin, an ionophore mediating electroneutral Na+/H+ exchange, supported growth of the mutant at unfavourable Na+/K+ ratios. Measurement of internal Na+ and K+ contents of wild-type and mutant cells revealed a decreased Na+/K+ ratio in mutant cells pre-incubated at a low external Na+/K+ ratio, while it remained at the level of the wild type after pre-incubation at a high external Na+/K+ ratio. We conclude that the Sll0273 protein is required for Na+ influx, especially at low external Na+ concentrations or low Na+/K+ ratios. This system may be part of a sodium cycle and may permit re-entry of Na+ into the cells, if nutrient/Na+ symporters are not functional or operating.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2000.00565.x
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  • 3
    Electronic Resource
    Electronic Resource
    A preliminary evaluation of professional accounting services: direct marketing on the Internet (2000)
    Bradford : Emerald
    The @journal of services marketing 14 (2000), S. 595-605 
    Details
    ISSN: 0887-6045
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Recent advances in the Internet have afforded professional accounting firms a form of promotion to enhance their image as technologically competent. Currently, over 2,000 accounting firms have Web sites registered with The List of CPA Firms Directory. The objective of this paper is to conduct a preliminary content analysis of the web pages of accounting firms. A total of 346 Web sites were randomly selected. The contents of the Web pages were examined and analysed for inclusion of client choice factors, basic company information, value added features, and graphics. The registration of the accounting firms' Web sites by search engines, Web yellow pages, CPA directories, and state CPA societies was also investigated. Generally, firms did a good job of providing basic client desired information about the firm, but they must register their Web sites with search engines that are commonly available and must encourage more interactivity with existing and potential clients by providing free information such as newsletters and links to relevant Web sites. They need to develop greater expertise in using the specific features and advantages of the Internet medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/08876040010352763
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  • 4
    Electronic Resource
    Electronic Resource
    Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp. PCC 6803 (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 227 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The iron deficiency-dependent regulation of isiAB transcription in Synechocystis sp. PCC 6803 was analyzed by fusion of modified isiAB promoter fragments to gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic fur mutant a corresponding incomplete repression of isiAB transcription under iron-replete conditions confirmed the role of Fur in isiAB regulation. However, a 90 bp region upstream of the putative −35 box of the isiAB promoter was essential for full promoter activity under iron-deplete conditions. This pattern indicates a dual promoter regulation by both a repressing mechanism exhibited via the Fur system and an unknown activating mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00689-X
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  • 5
    Electronic Resource
    Electronic Resource
    The iron-regulated isiA gene of Fischerella muscicola strain PCC 73103 is linked to a likewise regulated gene encoding a Pcb-like chlorophyll-binding protein (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 197 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The expression of the chlorophyll a-binding, iron stress-induced protein IsiA is part of the cyanobacterial response to iron deficiency. A new isiA gene from the filamentous heterocystous cyanobacterial strain, Fischerella muscicola PCC 73103, was identified using standard and inverse PCR. While in unicellular cyanobacterial strains isiA is organized in an operon with isiB (encoding flavodoxin), in Fischerella not an isiB gene but another chlorophyll-binding protein encoding gene was identified downstream of isiA, which shows significant similarities to Pcb-like protein encoding genes known from prochlorophytes. The expression of both genes was clearly activated under iron deficiency. Although isiA and pcbC were independently transcribed, the size of the pcbC transcript indicates a large iron-regulated operon. Beside a 10-fold increase of isiA transcript content iron-starved cells of Fischerella showed a blue-shift in the red chlorophyll a absorption peak. In addition, chlorophyll fluorescence at 77 K was dominated by an emission peak at 685 nm. These features are in accordance with the characteristics of IsiA accumulation in iron-starved unicellular cyanobacteria, suggesting identical IsiA function in heterocystous strains in spite of different genetic organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10593.x
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  • 6
    Electronic Resource
    Electronic Resource
    Mucosal vaccination with a recombinant OprF-I vaccine of Pseudomonas aeruginosa in healthy volunteers: comparison of a systemic vs. a mucosal booster schedule (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 37 (2003), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We compared the immunogenicity of two vaccination schedules with either a systemic or a mucosal booster, both following a mucosal primary vaccination with a recombinant outer membrane fusion protein of Pseudomonas aeruginosa (OprF-I) in 12 healthy volunteers. The systemic booster induced higher levels of OprF-I-specific serum antibodies of IgG isotype, with a mean±S.E.M. of 32.6±7.8×107 enzyme-linked immunosorbent assay (ELISA) units (EU) as compared to the nasal booster with 14.6±2.1×107 EU (P=0.05). Specific serum IgA antibodies and antibodies in saliva did not differ between the two vaccination groups. We conclude that a combined mucosal/systemic vaccination with the OprF-I vaccine may offer an enhanced systemic immunogenicity. Further studies on the long-term immunogenicity and induction of antibodies on the respiratory airway surface are warranted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0928-8244(03)00094-4
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  • 7
    Electronic Resource
    Electronic Resource
    Synthesis of the compatible solutes glucosylglycerol and trehalose by salt-stressed cells of Stenotrophomonas strains (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 243 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this study, physiological processes were analysed, which are involved in salt acclimation of two Stenotrophomonas species, Stenotrophomonas maltophilia strain DSM 50170 and Stenotrophomonas rhizophila strain DSM 14405. S. maltophilia accumulated trehalose as the only osmolyte, whereas S. rhizophila produced additionally to trehalose glucosylglycerol (GG). The different spectrum and amounts of compatible solutes in these two strains led to differences in terms of their salt tolerance. The human-associated S. maltophilia was able to grow in media containing up to 3% NaCl (w/v). In contrast, S. rhizophila propagated in salinities up to 5% NaCl (w/v). The strain was isolated from the rhizosphere, a microenvironment which is characterised by high and changing salinities. Light microscopic analysis of S. rhizophila cells showed a significant increase in cell length of salt-treated cells in comparison to control cells. Cells of S. rhizophila exposed to more than 2% NaCl excreted GG into the medium during the transition from exponential to stationary growth phase, while the internal trehalose pool remained constant. This feature offers a high potential for the biotechnological production of GG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2004.12.005
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  • 8
    Electronic Resource
    Electronic Resource
    Wobbling phonon excitations in strongly deformed triaxial nuclei (2003)
    Springer
    The European physical journal 20 (2003), S. 183-188 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. The wobbling mode is uniquely related to triaxiality and introduces a series of bands with increasing wobbling phonon number, $n_{\rm w}$ . The pattern of $\gamma$ -transitions between the wobbling excitations will be influenced by the presence of an aligned particle. Evidence for the wobbling mode was obtained recently, and even a two-phonon wobbling excitation has now been identified in 163 Lu. The similarity of the data in 163 Lu to new strongly deformed triaxial bands and connecting transitions in the neighbouring nuclei, 165 Lu and 167 Lu, establishes wobbling as a more general phenomenon in this region. The higher phonon wobbling excitations show some anharmonicity, but the characteristic large $\Delta n_{\rm w} = 1$ E2 strength is observed. The wobbling interpretation is based on the comparison of electromagnetic decay strength between bands to particle-rotor calculations in which an i 13/2 proton is coupled to a triaxial core.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1140/epja/i2002-10349-4
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  • 9
    Electronic Resource
    Electronic Resource
    Study of 169Hf at high rotational frequency (2001)
    Springer
    The European physical journal 12 (2001), S. 15-28 
    Details
    ISSN: 1434-601X
    Keywords: PACS. 21.10.-k Properties of nuclei; nuclear energy levels – 23.20.Lv Gamma transitions and level energies – 25.70.-z Low and intermediate energy heavy-ion reactions – 27.70.+q 150 ?A? 189
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. High-spin properties of the nucleus 169Hf have been studied through the fusion evaporation reaction 96Zr(76Ge,3n)169Hf at a beam energy of 310 MeV. The known rotational bands have been extended considerably and 6 new bands have been established, four of which form coupled bands with pronounced M1 connections. Quasiparticle assignments are suggested for the new band structures, and it appears that coupling to vibrational degrees of freedom plays a role. Both coupled bands involve the excitation of quasiprotons. In the region of highest spin, a large alignment gain is interpreted in terms of a mixed crossing where an h 9/2 and an h 11/2 quasiproton provide the two signatures of the aligning configuration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100500170035
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  • 10
    Electronic Resource
    Electronic Resource
    Ancient and Recent Horizontal Invasions of Drosophilids by P Elements (2000)
    Springer
    Journal of molecular evolution 51 (2000), S. 577-586 
    Details
    ISSN: 1432-1432
    Keywords: Key words:P element — Transposon evolution — Horizontal transfer —Drosophila—Scaptomyza—Lordiphosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. P elements of two different subfamilies designated as M- and O-type are thought to have invaded host species in the Drosophila obscura group via horizontal transmission from external sources. Sequence comparisons with P elements isolated from other species suggested that the horizontal invasion by the O-type must have been a rather recent event, whereas the M-type invasion should have occurred in the more distant past. To trace the phylogenetic history of O-type elements, additional taxa were screened for the presence of O- and M-type elements using type-specific PCR primers. The phylogeny deduced from the sequence data of a 927-bp section (14 taxa) indicate that O-type elements have undergone longer periods of regular vertical transmission in the lineages of the saltans and willistoni groups of Drosophila. However, starting from a species of the D. willistoni group they were transmitted horizontally into other lineages. First the lineage of the D. affinis subgroup was infected, and finally, in a more recent wave of horizontal spread, species of three different genera were invaded by O-type elements from the D. affinis lineage: Scaptomyza, Lordiphosa, and the sibling species D. bifasciata/D. imaii of the Drosophila obscura subgroup. The O-type elements isolated from these taxa are almost identical (sequence divergence 〈1%). In contrast, no such striking similarities are observed among M-type elements. Nevertheless, the sequence phylogeny of M-type elements is also not in accordance with the phylogeny of their host species, suggesting earlier horizontal transfer events. The results imply that P elements cross species barriers more frequently than previously thought but require a particular genomic environment and thus seem to be confined to a rather narrow spectrum of host species. Consequently, different P element types acquired by successive horizontal transmission events often coexist within the same genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002390010121
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