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  • Weitere Quellen  (6)
  • Oxford University Press  (5)
  • American Institute of Physics
  • 2005-2009  (3)
  • 2000-2004  (3)
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  • 1
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    Oxford University Press
    In:  Database: The Journal of Biological Databases and Curation, 2009 . bap009.
    Publikationsdatum: 2019-01-21
    Beschreibung: As ecosystem engineers, seagrasses are angiosperms of paramount ecological importance in shallow shoreline habitats around the globe. Furthermore, the ancestors of independent seagrass lineages have secondarily returned into the sea in separate, independent evolutionary events. Thus, understanding the molecular adaptation of this clade not only makes significant contributions to the field of ecology, but also to principles of parallel evolution as well. With the use of Dr. Zompo, the first interactive seagrass sequence database presented here, new insights into the molecular adaptation of marine environments can be inferred. The database is based on a total of 14 597 ESTs obtained from two seagrass species, Zostera marina and Posidonia oceanica, which have been processed, assembled and comprehensively annotated. Dr. Zompo provides experimentalists with a broad foundation to build experiments and consider challenges associated with the investigation of this class of non-domesticated monocotyledon systems. Our database, based on the Ruby on Rails framework, is rich in features including the retrieval of experimentally determined heat-responsive transcripts, mining for molecular markers (SSRs and SNPs), and weighted key word searches that allow access to annotation gathered on several levels including Pfam domains, GeneOntology and KEGG pathways. Well established plant genome sites such as The Arabidopsis Information Resource (TAIR) and the Rice Genome Annotation Project are interfaced by Dr. Zompo. With this project, we have initialized a valuable resource for plant biologists in general and the seagrass community in particular. The database is expected to grow together with more data to come in the near future, particularly with the recent initiation of the Zostera genome sequencing project. The Dr. Zompo database is available at http://drzompo.uni-muenster.de/
    Materialart: Article , PeerReviewed
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    Standort Signatur Erwartet Verfügbarkeit
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  • 2
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    Oxford University Press
    In:  FEMS Microbiology Ecology, 60 (2). pp. 177-188.
    Publikationsdatum: 2019-09-24
    Beschreibung: We studied the effect of the quorum-sensing (QS) blockers 5-hydroxy-3[(1R)-1-hydroxypropyl]-4-methylfuran-2(5H)-one (FUR1), (5R)-3,4-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]furan-2(5H)-one (FUR2) and triclosan (TRI) on the formation of bacterial biofilms, and the effect of these biofilms on the larval attachment of the polychaete Hydroides elegans and the bryozoan Bugula neritina. 14-day-old subtidal biofilms were harvested from artificial substrata and were allowed to develop in the laboratory with and without QS blockers. QS blockers inhibited the production of violacein by the QS reporter strain Chromobacterium violaceum CV026 and did not affect the metabolic activity of bacteria in multispecies biofilms. At a concentration of 10(-3) M all three tested compounds inhibited the establishment of microbial communities, but at one of 10(-4) M only FUR2 inhibited establishment. The tested QS blockers caused changes in bacterial density and bacterial community structure, as revealed by terminal restriction fragment length polymorphism and FISH. The groups most affected by QS blockers were Alphaproteobacteria, Gammaproteobacteria and the Cytophagales. Larvae of H. elegans and B. neritina avoided settling on biofilms that had developed in the presence of QS blockers. Our results suggest that QS blockers directly control the formation of multi-species biofilms, and indirectly - by means of biofilm properties - affect larval attachment on these modified biofilms.
    Materialart: Article , PeerReviewed
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    Standort Signatur Erwartet Verfügbarkeit
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  • 3
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    Oxford University Press
    In:  FEMS Microbiology Letters, 197 . pp. 171-178.
    Publikationsdatum: 2019-08-05
    Beschreibung: A PCR protocol for the detection of sulfur-oxidizing bacteria based on soxB genes that are essential for thiosulfate oxidation by sulfur-oxidizing bacteria of various phylogenetic groups which use the 'Paracoccus sulfur oxidation' pathway was developed. Five degenerate primers were used to specifically amplify fragments of soxB genes from different sulfur-oxidizing bacteria previously shown to oxidize thiosulfate. The PCR yielded a soxB fragment of approximately 1000 bp from most of the bacteria. Amino acid and nucleotide sequences of soxB from reference strains as well as from new isolates and environmental DNA from a hydrothermal vent habitat in the North Fiji Basin were compared and used to infer relationships of soxB between sulfur-oxidizing bacteria belonging to various 16S rDNA-based phylogenetic groups. Major phylogenetic lines derived from 16S rDNA were confirmed by soxB phylogeny. Thiosulfate-oxidizing green sulfur bacteria formed a coherent group by their soxB sequences. Likewise, clearly separated branches demonstrated the distant relationship of representatives of alpha-, beta-, and gamma-Proteobacteria including representative species of the former genus Thiobacillus (now Halothiobacillus - gamma-Proteobacteria, Thiobacillus - beta-Proteobacteria and Starkeya - alpha-Proteobacteria). This general picture emerged although apparent evidence for lateral transfer of the soxB gene is indicated and comparison of soxB phylogeny and 16S rDNA phylogeny points to the significance of this gene transfer in hydrothermal vent bacterial communities of the North Fiji Basin.
    Materialart: Article , PeerReviewed
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    Standort Signatur Erwartet Verfügbarkeit
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  • 4
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    American Institute of Physics
    In:  The Leading Edge, 21 (7). pp. 686-689.
    Publikationsdatum: 2016-07-13
    Beschreibung: As offshore petroleum exploration and development move into deeper water, industry must contend increasingly with gas hydrate, a solid compound that binds water and a low-molecular-weight gas (usually methane). Gas hydrate has been long studied in industry from an engineering viewpoint, due to its tendency to clog gas pipelines. However, hydrate also occurs naturally wherever there are high pressures, low temperatures, and sufficient concentrations of gas and water. These conditions prevail in two natural environments, both of which are sites of active exploration: permafrost regions and marine sediments on continental slopes. In this article we discuss seismic detection of gas hydrate in marine sediments. Gas hydrate in deepwater sediments poses both new opportunities and new hazards. An enormous quantity of natural gas, likely far exceeding the global inventory of conventional fossil fuels, is locked up worldwide in hydrates. Ex-traction of this unconventional resource presents unique exploration, engineering, and economic challenges, and several countries, including the United States, Japan, Canada, India, and Korea, have initiated joint industry-academic-governmental programs to begin studying those challenges. Hydrates also constitute a potential drilling hazard. Because hydrates are only stable in a restricted range of pressure and temperature, any activity that sufficiently raises temperature or lowers pressure could destabilize them, releasing potentially large volumes of gas and decreasing the shear strength of the host sediments. Assessment of the opportunities and hazards associated with hydrates requires reliable methods of detecting hydrate and accurate maps of their distribution and concentration. Hydrate may occur only within the upper few hundred meters of deepwater sediment, at any depth between the seafloor and the base of the stability zone, which is controlled by local pressure and temperature. Hydrate is occasionally exposed at the seafloor, where it can be detected either visually or acoustically by strong seismic reflection amplitudes or high backscatter …
    Materialart: Article , PeerReviewed
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    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2020-07-31
    Beschreibung: Microbial isolate Z143-1 found to be associated with an unidentified tunicate was characterized due to its significant antimicrobial activity. Z143-1 is similar to Pseudovibrio ascidiaceicola and Pseudovibrio denitrificans in morphological, physiological and biochemical characteristics, except for its ability to ferment glucose and produce a characteristic red pigment. Fatty acid methyl ester analysis revealed a predominance of the fatty acid 18:1 ω7c at 80.55%, at levels slightly lower than the Pseudovibrio denitrificans type strain DN34T (87.7%). The mol% G+C of Z143-1 is 54.02, relatively higher than the Pseudovibrio denitrificans type strain DN34T and Pseudovibrio ascidiaceicola with mol% G+C of 51.7 and 51.4, respectively. However, phylogenetic analysis of the 16S rRNA gene sequence of Z143-1 showed 100% similarity with the Pseudovibrio denitrificans type strain DN34T. In this study, the bacterium Z143-1 is reported as a new strain of Pseudovibrio denitrificans. While there is no report of a secondary metabolite for Pseudovibrio denitrificans, Z143-1 produces the red pigment heptylprodigiosin, also known as 16-methyl-15-heptyl-prodiginine, which shows anti-Staphylococcus aureus activity.
    Materialart: Article , PeerReviewed
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    Standort Signatur Erwartet Verfügbarkeit
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  • 6
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    Oxford University Press
    In:  ICES Journal of Marine Science, 61 (3). pp. 430-440.
    Publikationsdatum: 2021-09-03
    Beschreibung: A bioenergetic model for two narwhal (Monodon monoceros) sub-populations was developed to quantify daily gross energy requirements and estimate the biomass of Greenland halibut (Reinhardtius hippoglossoides) needed to sustain the sub-populations for their 5-month stay on wintering grounds in Baffin Bay. Whales in two separate wintering grounds were estimated to require 700 tonnes (s.e. 300) and 90 tonnes (s.e. 40) of Greenland halibut per day, assuming a diet of 50% Greenland halibut. Mean densities and length distributions of Greenland halibut inside and outside of the narwhal wintering grounds were correlated with predicted whale predation levels based on diving behavior. The difference in Greenland halibut biomass between an area with high predation and a comparable area without whales, approximately 19 000 tonnes, corresponded well with the predicted biomass removed by the narwhal sub-population on a diet of 50–75% Greenland halibut.
    Materialart: Article , PeerReviewed
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    Standort Signatur Erwartet Verfügbarkeit
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