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  • Springer  (36)
  • National Academy of Sciences  (6)
  • 2005-2009  (10)
  • 2000-2004  (18)
  • 1990-1994  (11)
  • 1980-1984  (1)
  • 1970-1974  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Plane elastic solution for the second mixed boundary value problem and its application (1994)
    Springer
    Archive of applied mechanics 64 (1994), S. 295-306 
    Details
    ISSN: 1432-0681
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Übersicht Die Lösung des zweiten gemischten Randwertproblems der ebenen Elastizität mit zwei kraftbeanspruchten und zwei gestützten Rändern wird dargestellt. Für die Analyse wird eine rationale Abbildung verwendet, die als Summe partieller Lösungen aus komplexen Spannungsfunktionen aufgebaut ist. Für gleichmäßige Zug- und Schubbeanspruchungen kann die Lösung in geschlossener Form dargestellt werden und ist verwendbar in Rißaufgaben der linear elastischen Bruchmechanik. Als Illustration der allgemeinen Lösung dient ein verzweigter Bereich der gegenseitigen Einwirkung einer quadratischen Öffnung mit Seitenkerbe und eines Risses. Die Spannungsverteilungen und Spannungsintensitäten werden dabei berechnet.
    Notes: Summary A solution is obtained for the second mixed boundary value problem with two boundaries loaded with external forces, and the other two boundaries simply supported. A rational mapping function formed as a sum of fractional expressions and complex stress functions is used for the analysis. A closed form solution can be obtained under uniform tensile and shearing loadings. These solutions are applicable to crack problems in linear elastic fracture mechanics. In order to illustrate the general solution, a double connected region is considered, in which interaction between a square hole with a crack extension and another crack is investigated. Stress distributions and stress intensity factors are obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00788215
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  • 2
    Electronic Resource
    Electronic Resource
    Thermal calculation of the hot rolling process of sheet aluminium (1992)
    Springer
    Archive of applied mechanics 62 (1992), S. 435-446 
    Details
    ISSN: 1432-0681
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Übersicht Vorgestellt wird ein numerisches Modell zur Ermittlung der Walzguttemperatur in einer Warmbandstraße für Aluminium, das auf einfachen theoretischen Grundannahmen beruht. Mit dem Modell wird die Möglichkeit der Produktivitätssteigerung durch eine veränderte Gestaltung der Fertigstraße unter dem Gesichtspunkt der Steuerung der Walzguttemperatur untersucht. Die Ergebnisse zeigen, daß die Erhöhung der Anzahl der Fertiggerüste einen positiven Einfluß auf die Produktivität hat.
    Notes: Summary In order to predict the material temperature throughout the hot rolling process of aluminium, a numerical model is proposed in a theoretical as well as simple way. Using the model, the possibility of the improvement in productivity by reforming the finishing mill arrangement is examined under aspect of the control of material temperature. The results so obtained show the positive effect of increasing the number of finishing mills on the productivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00810234
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  • 3
    Electronic Resource
    Electronic Resource
    Polychlorinated Dibenzo-p-Dioxins (PCDDs), Dibenzofurans (PCDFs), Biphenyls (PCBs), and Organochlorine Pesticides in Yellow-Blotched Map Turtle from the Pascagoula River Basin, Mississippi, USA (2000)
    Springer
    Archives of environmental contamination and toxicology 38 (2000), S. 362-370 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract. Concentrations of polychlorinated-dibenzo-p-dioxins (PCDDs), -dibenzofurans (PCDFs), -biphenyls (PCBs), and organochlorine pesticides were measured in tissues of map turtles collected from two locations in the Pascagoula River drainage of Mississippi, USA. PCBs were most predominant among the organochlorines with a concentration of up to 99 ng/g, wet weight (580 ng/g, lipid weight) in livers. The greatest concentration of PCDDs/DFs of 1.1 pg/g, wet weight (15.76 pg/g, lipid weight) was found in the liver of a male turtle. The measured concentrations of organochlorines were less than those reported for turtles from the Great Lakes Basin and upper St. Lawrence River. PCBs contributed 90–99% of the total estimated 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (TEQs). Particularly, PCB congeners 105, 118, and 156 accounted for 68–80% of the estimated toxic potency of PCBs in turtles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002449910048
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  • 4
    Electronic Resource
    Electronic Resource
    The substrate specificity of maleate hydratase from Arthrobacter sp. strain MCI2612 (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 215-218 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The substrate specificity of maleate hydratase from Arthrobacter sp. strain MCI2612 was examined with maleate and its derivatives. Maleate hydratase was shown to catalyze the hydration of maleate, chloromaleate, bromomaleate, and citraconate. Water was added trans to chloromaleate and bromomaleate to synthesize the (−)-erythro-β-substituted derivatives of d-malate. (R)-(−)-Citramalate was synthesized from citraconate by using maleate hydratase. Many organic acids such as acetylenedicarboxylate, l(+)-, d(−)-, and meso-tartarate, and cis-, trans-epoxysuccinate inhibited competitively the formation of d-malate from maleate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186962
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  • 5
    Electronic Resource
    Electronic Resource
    The substrate specificity of maleate hydratase from Arthrobacter sp. strain MCI2612 (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 215-218 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The substrate specificity of maleate hydratase from Arthrobacter sp. strain MCI2612 was examined with maleate and its derivatives. Maleate hydratase was shown to catalyze the hydration of maleate, chloromaleate, bromomaleate, and citraconate. Water was added trans to chloromaleate and bromomaleate to synthesize the (−)-erythro-β-substituted derivatives of D-malate. (R)-(−)-Citramalate was synthesized from citraconate by using maleate hydratase. Many organic acids such as acetylenedicarboxylate, L(+)-, D(−)-, and meso-tartarate, and cis-, trans-epoxysuccinate inhibited competitively the formation of D-malate from maleate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050133
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  • 6
    Electronic Resource
    Electronic Resource
    Construction of an engineered yeast with glucose-inducible emission of green fluorescence from the cell surface (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 90-96 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An engineered yeast with emission of fluorescence from the cell surface was constructed. Cell surface engineering was applied to display a visible reporter molecule, green fluorescent protein (GFP). A glucose-inducible promoter GAPDH as a model promoter was selected to control the expression of the reporter gene in response to environmental changes. The GFP gene was fused with the gene encoding the C-terminal half of α-agglutinin of Saccharomyces cerevisiae having a glycosylphosphatidylinositol anchor attachment signal sequence. A secretion signal sequence of the fungal glucoamylase precursor protein was connected to the N-terminal of GFP. This designed gene was integrated into the TRP1 locus of the chromosome of S. cerevisiae with homologous recombination. Fluorescence microscopy demonstrated that the transformant cells emitted green fluorescence derived from functionally expressed GFP involved in the fusion molecule. The surface display of GFP was further verified by immunofluorescence labeling with a polyclonal antibody (raised in rabbits) against GFP as the first antibody and Rhodamine Red-X-conjugated goat anti-rabbit IgG as the second antibody which cannot penetrate into the cell membrane. The display of GFP on the cell surface was confirmed using a confocal laser scanning microscope and by measuring fluorescence in each cell fraction obtained after the subcellular fractionation. As GFP was proved to be displayed as an active form on the cell surface, selection of promoters will endow yeast cells with abilities to respond to changes in environmental conditions, including nutrient concentrations in the media, through the emission of fluorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002539900307
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  • 7
    Electronic Resource
    Electronic Resource
    Langerhans cells in human allergic contact dermatitis contain varying numbers of birbeck granules (1990)
    Springer
    Histochemistry and cell biology 94 (1990), S. 497-504 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dynamic changes in human Langerhans cells (LCs) were studied with OK T6, anti-HLA-DR antibody, and Lag antibody in allergic contact dermatitis (ACD). Both T6-positive (T6+) cells and Lag-positive (Lag+) cells in the epidermis decreased in number from 0 to 48 h, but then gradually increased after day 7 of ACD. Lag+ cells after day 7 manifested a variety of staining intensities from weak to strong. It was also shown, after day 7, that some T6+ cells were Lag negative whereas all Lag+ cells were T6 positive. Flow cytometric analysis suggested that Lag-strongly-positive cells and Lag-weakly-positive cells belonged to the same population, and that the relative amount of Lag antigens in T6+ LCs gradually increased after day 7. Immunoelectron microscopy revealed that the Lag-strongly-positive cells contained numerous Lag-reactive Birbeck granules (BGs) whereas the Lag-weakly-positive cells contained fewer BGs in the cytoplasm. In some Lag-weakly-positive cells, no BGs were detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272613
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  • 8
    Electronic Resource
    Electronic Resource
    Microtubule-dependent migration of the cell nucleus toward a future leading edge in amoebae ofPhysarum polycephalum (2000)
    Springer
    Protoplasma 211 (2000), S. 172-182 
    Details
    ISSN: 1615-6102
    Keywords: Centrosome ; Chemotaxis ; Microtubules ; Nucleus ; Pseudopod
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In several cell types, an intriguing correlation exists between the position of the centrosome and the direction of cell locomotion. The centrosome is positioned between the leading edge pseudopod and the nucleus. This suggests that the polarized distribution of organelles in the cytoplasm is coupled spatially with structural and functional polarity in the cell cortex. To study cellular polarization with special interest in the roles of microtubules, we have analyzed the effects of microtubule-disrupting reagents and local laser irradiation on behaviors of both the nucleus and the centrosome in living amoebae ofPhysarum polycephalum. Physarum cells often have 2–3 pseudopods. One of the pseudopods keeps extending to become a stable leading edge while the rest retracts, a crucial step that reorients cells during locomotion. The nucleus, together with the centrosome, moves specifically toward the pseudopod that will become the leading edge. Disruption of microtubules with nocodazole randomizes positions of the nucleus, indicating the involvement of microtubules in the directional migration of the nucleus toward a specific pseudopod. The migration direction of the nucleus is reversed immediately after the UV laser is irradiated at regions between the nucleus and the future leading pseudopod. In contrast, irradiation at regions between the future tail and the nucleus does not affect nuclear migration. By immunofluorescence, we confirmed fragmentation of microtubules specifically in the irradiated region. These results suggest that the nucleus is pulled together with the centrosome toward the future leading-edge pseudopod in a microtubule-dependent manner. Microtubules seem to exert the pulling force generated in the cell cortex on the centrosome. They may serve as a mediator of shape changes initiated in the cell cortex to the organelle geometry in the endoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01304485
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  • 9
    Electronic Resource
    Electronic Resource
    Vergleich der Fraktionierungswirksamkeiten der Lösungs- und Fällungs-Chromatographie bei der Polymerfraktionierung (1974)
    Springer
    Colloid & polymer science 252 (1974), S. 177-177 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01555627
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  • 10
    Electronic Resource
    Electronic Resource
    Edge density profile variation during a magnetic axis shift in the CHS heliotron/torsatron (1993)
    Springer
    Journal of fusion energy 12 (1993), S. 273-279 
    Details
    ISSN: 1572-9591
    Keywords: Edge density profile ; magnetic axis shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Edge density profiles were measured during natural and forced magnetic axis shifts in the lowaspect-ratio heliotron/torsatron CHS, using an 8 keV fast neutral lithium beam probe. The Shafranov shift of the magnetic axis (and hence the dislocation of the LCFS) that was negligible for a low density ECH discharge became substantial (∼4.5 cm) for a high density NBI discharge (〈n e〉∼2.5× 1013cm−3) and the corresponding radius of the LCFS increased about 1.2 cm in major radius compared to the vacuum case. For NBI discharges with different settings of the vacuum magnetic axisR ax (fixed during the discharge), the measured edge density profiles indicated reasonable agreement between the theoretically and experimentally obtained LCFS radii for 90 cm 〈R ax〈101.6cm, while forR ax〈90 cm the measured radius was 10% larger than expected. When a change ofR ax from 94.7 cm to 89.9 cm during a discharge was imposed externally, a well behaved plasma boundary moved inward smoothly by about 7.5 cm, while the steepness of the edge density profile changed for different values ofR ax. The steepest profile was attained forR ax=92.1 cm when the highest energy content and average density were achieved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01079670
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