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  • Artikel  (371)
  • Chemistry  (359)
  • Hansenula polymorpha  (5)
  • Biochemistry and Biotechnology
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  • Liquid chromatography
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  • 1
    Digitale Medien
    Digitale Medien
    Regulation of the synthesis of methanol oxidizing enzymes inKloeckera sp. 2201 andHansenula polymorpha, a comparison (1982)
    Springer
    Archives of microbiology 131 (1982), S. 174-175 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Hansenula polymorpha ; Kloeckera sp. 2201 ; Methanol ; Glucose ; Methanol dissimilating enzymes ; Regulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A comparative study was made of the regulation of the synthesis of methanol dissimilating enzymes inkloeckera sp. 2201 andHansenula polymorpha using chemostat and batch growth conditions and methanol or glucose as carbon sources. During growth in methanol-limited chemostat cultures similar enzyme patterns for alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase in the two yeasts were found. When growing in batch culture with glucoseH. polymorpha, but notKloeckera sp. 2201, was found to produce ethanol which might affect the synthesis of these enzymes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01054002
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  • 2
    Digitale Medien
    Digitale Medien
    The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri (1983)
    Springer
    Cell & tissue research 233 (1983), S. 377-388 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Gonadotrops ; Gonadotropin secretion ; Steroids ; Brain-pituitary-gonadal axis ; Juvenile teleost
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In a cytophysiological study it was investigated whether in juvenile trout gonadal steroids stimulate the gonadotropic (GTH)-cells directly or indirectly via the brain. Pituitaries of donor animals were transplanted into the caudal musculature of testosterone-treated and non-testosterone-treated host fish. Testosterone treatment caused an increase in GTH-content in the in situ pituitaries and in the grafts. Accordingly, the gonadotrops displayed ultrastructural changes such as the appearance of well-developed Golgi systems and large globules. The stimulation of the morphological development of gonadotrops and of synthesis and storage of GTH in the allografted pituitaries indicates that testosterone affects the GTH-cells directly. In untreated juvenile trout the gonadotropin content of the pituitary and the gonadotropin concentration in the plasma vary with the time of year. This variation and the role of testosterone and gonadotropin-releasing hormone on the release of GTH are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00238304
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  • 3
    Digitale Medien
    Digitale Medien
    Spektroskopische Untersuchungen zur Acidität von Dithiophosphorsäure-O,O′-estern in unpolaren, aprotischen Lösungsmitteln (1995)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 337 (1995), S. 245-249 
    Details
    ISSN: 0941-1216
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Spectroscopic Investigations on the Acidity of O,O′-Dithiophosphoric Acid Esters in Nonpolar Aprotic Solvents
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prac.19953370153
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  • 4
    Digitale Medien
    Digitale Medien
    Über langkettige Sulfonamide und ihre therapeutischen Eigenschaften (1942)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 75 (1942), S. 369-378 
    Details
    ISSN: 0365-9631
    Schlagwort(e): Chemistry ; Inorganic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cber.19420750412
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  • 5
    Digitale Medien
    Digitale Medien
    Die Polymerisation des Isoprens durch Bleicherden; Darstellung eines aliphat. Diisoprens. (Über Polymerisationsreaktionen von Dienen, V. MitteilungIV. Mitteil.: Th. Lennartz, B. 76, 1006 [1943].) (1943)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 76 (1943), S. 1161-1165 
    Details
    ISSN: 0365-9631
    Schlagwort(e): Chemistry ; Inorganic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cber.19430761202
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  • 6
    Digitale Medien
    Digitale Medien
    Mixed substrate growth of methylotrophic yeasts in chemostat culture: Influence of the dilution rate on the utilisation of a mixture of glucose and methanol (1982)
    Springer
    Archives of microbiology 131 (1982), S. 8-13 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Hansenula polymorpha ; Kloeckera sp. 2201 ; Mixed substrate utilisation ; Chemostat ; Induction ; Repression ; Methanol ; Glucose
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The growth of Hansenula polymorpha and Kloeckera sp. 2201 with a mixture of glucose and methanol (38.8%/61.2%, w/w) and the regulation of the methanol dissimilating enzymes alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase were studied in chemostat culture, as a function of the dilution rate. Both organisms utilized and assimilated glucose and methanol simultaneously up to dilution rates of 0.30 h-1 (H. polymorpha) and 0.26h-1, respectively (Kloeckera sp. 2201) which significantly exceeded μmax found for the two yeasts with methanol as the only source of carbon. At higher dilution rates methanol utilisation ceased and only glucose was assimilated. Over the whole range of mixed-substrate growth both carbon sources were assimilated with the same efficiency as during growth with glucose or methanol alone. In cultures of H. polymorpha, however, the growth yield for glucose was lowered by the unmetabolized methanol at high dilution rates. During growth on both carbon sources the repression of the synthesis of all catabolic methanol enzymes which is normally caused by glucose was overcome by the inductive effect of the simultaneously fed methanol. In both organisms the synthesis of alcohol oxidase was found to be regulated differently as compared to catalase, formaldehyde and formate dehydrogenase. Whereas increasing repression of the synthesis of alcohol oxidase was found with increasing dilution rates as indicated by gradually decreasing specific activities of this enzyme in cell-free extracts, the specific activities of this enzyme in cell-free extracts, the specific activities of catalase and the dehydrogenases increased with increasing growth rates until repression started. The results indicate similar patterns of the regulation of the synthesis of methanol dissimilating enzymes in different methylotrophic yeasts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00451491
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  • 7
    Digitale Medien
    Digitale Medien
    Methanol metabolism in yeasts: Regulation of the synthesis of catabolic enzymes (1980)
    Springer
    Archives of microbiology 124 (1980), S. 115-121 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Derepression ; Catabolite inactivation ; Alcohol oxidase ; Catalase ; Formaldehyde dehydrogenase ; Formate dehydrogenase ; Hansenula polymorpha ; Kloeckera sp. 2201 ; Peroxisomes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The regulation of the synthesis of four dissimilatory enzymes involved in methanol metabolism, namely alcohol oxidase, formaldehyde dehydrogenase, formate dehydrogenase and catalase was investigated in the yeasts Hansenula polymorpha and Kloeckera sp. 2201. Enzyme profiles in cell-free extracts of the two organisms grown under glucose limitation at various dilution rates, suggested that the synthesis of these enzymes is controlled by derepression — represion rather than by induction — repression. Except for alcohol oxidase, the extent to which catabolite repression of the catabolic enzymes was relieved at low dilution rates was similar in both organisms. In Hansenula polymorpha the level of alcohol oxidase in the cells gradually increased with decreasing dilution rate, whilst in Kloeckera sp. 2201 derepression of alcohol oxidase synthesis was only observed at dilution rates below 0.10 h−1 and occurred to a much smaller extent than in Hansenula polymorpha. Derepression of alcohol oxidase and catalase in cells of Hansenula polymorpha was accompanied by synthesis of peroxisomes. Moreover, peroxisomes were degraded with a concurrent loss of alcohol oxidase and catalase activities when excess glucose was introduced into the culture. This process of catabolite inactivation of peroxisomal enzymes did not affect cytoplasmic formaldehyde dehydrogenase.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00427715
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  • 8
    Digitale Medien
    Digitale Medien
    Regulatory flexibility of methylotrophic yeasts in chemostat cultures: Simultaneous assimilation of glucose and methanol at a fixed dilution rate (1982)
    Springer
    Archives of microbiology 131 (1982), S. 1-7 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Hansenula polymorpha ; Kloeckera sp. 2201 ; Chemostat ; Mixed substrates ; Glucose ; Methanol ; Growth yields ; Enzyme regulation ; Dissimilatory enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The influence of the composition of methanol/glucose-mixtures as only sources of carbon and energy on growth and regulation of the synthesis of enzymes involved in methanol-dissimilation was studied under chemostat conditions at a fixed dilution rate with the methylotrophic yeasts Hansenula polymorpha and Kloeckera sp. 2201. Both carbon sources were found to be utilized completely independently of the composition of the C1/C6 mixture. Using mixtures of 14C-labelled methanol and glucose the growth yield for glucose was found to be constant for all C1/C6-mixtures tested and both yeasts. The growth yield for methanol, however, was reduced by up to 25% when the proportion of methanol in the inflowing medium was lower than 20% (w/w with respect to glucose) for H. polymorpha and 50% (w/w with respect to glucose) for Kloeckera sp. 2201 respectively. During growth with C1/C6-mixtures containing higher C1-proportions of methanol regular growth yields for methanol were recorded which corresponded to the growth yields found with methanol as the only carbon source. The regulation of the synthesis of the enzymes of the dissimilatory pathway for methanol was found to be under multiple control. Although glucose was present in the medium methanol had a positive effect on the synthesis of these enzymes. Thus, in addition to derepression induction by methanol was also observed. This inductive effect was found to increase with increasing proportions of methanol in the mixture. Depending on the enzyme, 10–40% methanol in the mixture resulted in a maximal induction with enzyme specific activities equal to those found in cells grown with methanol as the only carbon source. No further enhancements in enzyme specific activities were observed during growth on mixtures containing more than 40% methanol.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00451490
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  • 9
    Digitale Medien
    Digitale Medien
    Regulation of protein synthesis in methylotrophic yeasts: Repression of methanol dissimilating enzymes by nitrogen limitation (1982)
    Springer
    Archives of microbiology 131 (1982), S. 95-101 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Chemostat ; Nitrogen limitation ; Hansenula polymorpha ; Kloeckera sp. 2201 ; Carbon limitation ; Repression ; Methanol dissimilating enzymes ; Shift experiment ; Catabolite inactivation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The influence of nitrogen limitation on the regulation of the methanol oxidizing enzymes alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase in the two methylotrophic yeastsHansenula polymorpha andKloeckera sp. 2201 was studied in continuous culture. When shifted from carbon-limited growth conditions (with a mixture of glucose and methanol as carbon sources) to a nitrogen-limited environment both cultures were found to go through a transition phase where neither enhanced residual concentrations of the nitrogen source nor of one of the two carbon sources could be detected in the supernatant. As soon as nitrogen became a limiting substrate an immediate reorganisation of the cell composition was initiated: protein content of the cells dropped to approximately 40% of its initial value, glycogen was synthesized and the enzyme composition of the cells was changed. The peroxisomal enzymes alcohol oxidase and catalase in both organisms and the two dehydrogenases for formaldehyde and formate in cells ofKloeckera sp. 2201 were subject to degradation (catabolite inactivation). The measured rates of inactivation indicated that in cells ofH. polymorpha this process might be limited to peroxisomes, whereas inKloeckera sp. 2201 the degradation was found to affect peroxisomal as well as cytoplasmic enzymes. In contrast to methanol dissimilating enzymes the net rate of synthesis of hexokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase was not affected by this process but those enzymes were synthesized with increased rates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01053988
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  • 10
    Digitale Medien
    Digitale Medien
    Isolation of gonadotrops from the pituitary of the African catfish, Clarias lazera (1984)
    Springer
    Cell & tissue research 236 (1984), S. 669-675 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Gonadotrops ; Isolation ; Cell culture ; Gonadotropin secretion ; African catfish (Clarias lazera)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Dispersed pituitary cells from male African catfish, Clarias lazera, were fractionated in a density gradient of Percoll. Five fractions were isolated, consisting of about 6, 19, 39, 95 and 83% gonadotrops, respectively. The gonadotrops were identified by their ultrastructural characteristics, by immunocytochemistry, and by measuring their hormone content. After one day in culture, in each fraction the secretion of gonadotropin could be stimulated by a luteinizing hormone-releasing hormone analogue, indicating that the cells had retained their functional integrity. Since the regulatory mechanisms of different cell types from the pituitary have some similarity, purification of the gonadotrops provides a model to study the regulation of gonadotropin secretion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217237
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