Publikationsdatum:
2013-12-25
Beschreibung:
DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.
Print ISSN:
0305-1048
Digitale ISSN:
1362-4962
Thema:
Biologie
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