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  • 1
    Publication Date: 2012-06-13
    Description: Understanding the mechanisms that control processing of the amyloid precursor protein (APP) to produce amyloid-β (Aβ) peptide represents a key area of Alzheimer's disease research. Here, we show that siRNA-mediated loss of calsyntenin-1 in cultured neurons alters APP processing to increase production of Aβ. We also show that calsyntenin-1 is reduced in Alzheimer's disease brains and that the extent of this reduction correlates with increased Aβ levels. Calsyntenin-1 is a ligand for kinesin-1 light chains and APP is transported through axons on kinesin-1 molecular motors. Defects in axonal transport are an early pathological feature in Alzheimer's disease and defective APP transport is known to increase Aβ production. We show that calsyntenin-1 and APP are co-transported through axons and that siRNA-induced loss of calsyntenin-1 markedly disrupts axonal transport of APP. Thus, perturbation to axonal transport of APP on calsyntenin-1 containing carriers induces alterations to APP processing that increase production of Aβ. Together, our findings suggest that disruption of calsyntenin-1-associated axonal transport of APP is a pathogenic mechanism in Alzheimer's disease.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
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  • 2
    Publication Date: 2013-10-29
    Description: Disruption to axonal transport is an early pathological feature in Alzheimer's disease. The amyloid precursor protein (APP) is a key axonal transport cargo in Alzheimer's disease since perturbation of its transport increases APP processing and production of amyloid-β peptide (Aβ) that is deposited in the brains of Alzheimer's disease patients. APP is transported anterogradely through axons on kinesin-1 motors. One favoured route for attachment of APP to kinesin-1 involves the scaffolding protein c-Jun N-terminal kinase-interacting protein-1 (JIP1), which has been shown to bind both APP and kinesin-1 light chain (KLC). However, direct experimental evidence to support a role of JIP1 in APP transport is lacking. Notably, the effect of loss of JIP1 on movement of APP through axons of living neurons, and the impact of such loss on APP processing and Aβ production has not been reported. To address these issues, we monitored how siRNA mediated loss of JIP1 influenced transport of enhanced green fluorescent protein (EGFP)-tagged APP through axons and production of endogenous Aβ in living neurons. Surprisingly, we found that knockdown of JIP1 did not affect either APP transport or Aβ production. These results have important implications for our understanding of APP trafficking in Alzheimer's disease.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
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  • 3
    Publication Date: 2013-04-13
    Description: Silicon monoxide maser emission has been detected in the circumstellar envelopes of many evolved stars in various vibrationally excited rotational transitions. It is considered a good tracer of the wind dynamics close to the photosphere of the star. We have investigated the polarization morphology in the circumstellar envelope of an asymptotic giant branch star, R Cassiopeiae. We mapped the linear and circular polarization of SiO masers in the v  = 1, J  = 1–0 transition. The linear polarization is typically a few tens of per cent, while the circular polarization is a few per cent. The fractional polarization tends to be higher for emission of lower total intensity. We found that, in some isolated features, the fractional linear polarization appears to exceed 100 per cent. We found the Faraday rotation is not negligible but is ~15°, which could produce small-scale structure in polarized emission, whilst the total intensity is smoother and partly resolved out. The polarization angles vary considerably from feature to feature, but there is a tendency to favour the directions parallel or perpendicular to the radial direction with respect to the star. In some features, the polarization angle abruptly flips 90°. We found that our data are in the regime g  〉〉 R  〉〉 , which indicates that the model of Goldreich, Keeley & Kwan can be applied and the polarization angle flip is caused when the magnetic field is at close to 55° to the line of sight. The polarization angle configuration is consistent with a radial magnetic field, although other configurations are not excluded.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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