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  • Oxford University Press  (30)
  • BioMed Central  (27)
  • Nature Publishing Group
  • 2010-2014  (57)
  • 1955-1959  (5)
  • 1
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    Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein (2012)
    BioMed Central
    Details
    Publication Date: 2012-06-15
    Description: Background: Single-stranded DNA binding proteins (SSB) are essential for DNA replication, repair, and recombination in all organisms. SSB works in concert with a variety of DNA metabolizing enzymes such as DNA polymerase. Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations [less than or equal to]100 mug/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high affinity comparable to other prokaryotic SSBs. Thermodynamic analysis indicated both hydrophobic and ionic contributions to ssDNA binding. FRET analysis of oligo(dT)70 binding suggested that SSBBA forms a tetrameric assembly upon ssDNA binding. This report provides evidence of a bacterial SSB that utilizes a novel mechanism for DNA binding through the formation of a transient tetrameric structure. Conclusions: Unlike other prokaryotic SSB proteins, SSBBA from Bacillus anthracis appeared to be monomeric at concentrations [less than or equal to]100 mug/ml as determined by SE-HPLC. SSBBA retained its ability to bind ssDNA with very high affinity, comparable to SSB proteins which are tetrameric. In the presence of a long ssDNA template, SSBBA appears to form a transient tetrameric structure. Its unique structure appears to be due to the cumulative effect of multiple key amino acid changes in its sequence during evolution, leading to perturbation of stable dimer and tetramer formation. The structural features of SSBBA could promote facile assembly and disassembly of the protein-DNA complex required in processes such as DNA replication.
    Electronic ISSN: 1471-2091
    Topics: Chemistry and Pharmacology
    Published by BioMed Central
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  • 2
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    Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein (2012)
    BioMed Central
    Details
    Publication Date: 2012-01-01
    Electronic ISSN: 1471-2091
    Topics: Chemistry and Pharmacology
    Published by BioMed Central
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  • 3
    Electronic Resource
    Electronic Resource
    Chemical Nature of Nucleic Acids in Cyanophyceae (1956)
    [s.l.] : Nature Publishing Group
    Nature 177 (1956), S. 95-96 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The deoxyribonucleic acid separated from Nostoc muscorum gives a positive test with the Dische reagent, indicating the presence of deoxypentose-sugar. Chromatographic analysis of the hydrolysate according to the procedure of Wyatt2 revealed only three spots of bases, corresponding to guanine, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/177095b0
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Slow Disappearance of Fluorescence in Thionine on Addition of Abietic Acid (1955)
    [s.l.] : Nature Publishing Group
    Nature 176 (1955), S. 214-215 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] It can be seen from Fig. 1 (curves 1, 2 and 3) that the higher the concentration of the acid or the dye, the more rapid is the quenching. Curve 4 shows that a higher temperature brings about faster quenching, and curve 5 shows that the medium strongly influences the process, n-butyl alcohol ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/176214a0
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  • 5
    Electronic Resource
    Electronic Resource
    Surface-active Characteristics of Sodium Anacardate isolated from Cashew Nut Shell Oil (1958)
    [s.l.] : Nature Publishing Group
    Nature 182 (1958), S. 1299-1300 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Shell liquid extracted with 95 per cent ethyl alcohol was saponified and the non-saponifiable material was removed by extraction with ethyl ether. Stage-wise liberation of cardol and then of anacardic acid (followed by separate extractions with ethyl ether), was carried out by passing carbon ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1821299b0
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  • 6
    Electronic Resource
    Electronic Resource
    Thioctic Acid and Photosynthetic Fixation of Carbon Dioxide (1958)
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 1219-1220 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Seven-day old cultures of Chlorella pyrenoidosa and Sce ±edesmus bliquus were centrifuged down at 12 C. in an MSE centrifuge and resuspended in 10 ml. phosphate buffer of pH. 6-8. The cells were then incubated with different concentrations of thioctic acid and 30 JJLC. of sodium ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1811219a0
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  • 7
    Electronic Resource
    Electronic Resource
    Relationship between Auxins and Nucleic Acid Synthesis in Coleoptile Tissues (1959)
    [s.l.] : Nature Publishing Group
    Nature 183 (1959), S. 1824-1825 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Seeds of Avena sativa var. N.P.I and Oryza sativa var. Rupsail were used. The seeds were germinated in the dark with occasional red light for 3 days. The coleoptiles were then decapitated at 2-3 mm. and floated on water for 2 hr. to free them from any endogenous auxin. After a further decapitation ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1831824b0
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  • 8
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    Invaders' control on post-disturbance succession in coastal mangroves (2012)
    Oxford University Press
    Details
    Publication Date: 2012-04-13
    Description: Aims In recent years, coastal mangroves have been frequently affected by large disturbances (cyclones, hurricanes, flooding and tsunamis) and post-disturbance vegetation is often dominated by small stature mangrove, mangrove-associate and non-mangrove species potentially affecting ecosystem functioning. Knowledge on the processes of mangrove vegetation development and recovery (succession) following normal and large disturbances will benefit practitioners in designing robust ecosystem management/restoration plans. Here we propose a conceptual model of disturbance-mediated succession in mangroves. Methods Based on field observations and species’ life history traits, we develop conceptual models of mangrove succession under normal disturbance regime and recently experienced increased frequency of large disturbances. We evaluate our conceptual models by conducting a scenario testing experiment. Important Findings We suggest two predominant processes affecting mangrove succession after disturbance: propagule limitation due to damage of seed producing mature trees and dispersal barrier resulting from biological invasion associated with large disturbance. We argue that large disturbances affect mature trees more than the small-stature non-tree (shrubs, herbs and climbers) species creating a larger propagule shortage for mangrove tree species than non-tree species. Secondly, large disturbances facilitate invasion of free-floating aquatics, which may interfere with the flow-facilitated propagule dispersal and seedling establishment of mangrove species. In a scenario testing experiment, we have shown that similar levels of disturbance impact vegetation development and recovery differently depending on the presence or absence of invasive species. We conclude that since biological invasion is one of the major drivers of post-disturbance mangrove succession, the dimension of biological invasion should be included in prediction, management and restoration of mangrove forests.
    Print ISSN: 1752-993X
    Electronic ISSN: 1752-9921
    Topics: Biology
    Published by Oxford University Press
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  • 9
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    Functional evaluation of BRCA2 variants mapping to the PALB2-binding and C-terminal DNA-binding domains using a mouse ES cell-based assay (2012)
    Oxford University Press
    Details
    Publication Date: 2012-08-28
    Description: Single-nucleotide substitutions and small in-frame insertions or deletions identified in human breast cancer susceptibility genes BRCA1 and BRCA2 are frequently classified as variants of unknown clinical significance (VUS) due to the availability of very limited information about their functional consequences. Such variants can most reliably be classified as pathogenic or non-pathogenic based on the data of their co-segregation with breast cancer in affected families and/or their co-occurrence with a pathogenic mutation. Biological assays that examine the effect of variants on protein function can provide important information that can be used in conjunction with available familial data to determine the pathogenicity of VUS. In this report, we have used a previously described mouse embryonic stem (mES) cell-based functional assay to characterize eight BRCA2 VUS that affect highly conserved amino acid residues and map to the N-terminal PALB2-binding or the C-terminal DNA-binding domains. For several of these variants, very limited co-segregation information is available, making it difficult to determine their pathogenicity. Based on their ability to rescue the lethality of Brca2- deficient mES cells and their effect on sensitivity to DNA-damaging agents, homologous recombination and genomic integrity, we have classified these variants as pathogenic or non-pathogenic. In addition, we have used homology-based modeling as a predictive tool to assess the effect of some of these variants on the structural integrity of the C-terminal DNA-binding domain and also generated a knock-in mouse model to analyze the physiological significance of a residue reported to be essential for the interaction of BRCA2 with meiosis-specific recombinase, DMC1.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
    Published by Oxford University Press
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  • 10
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    Human iPSC models of neuronal ceroid lipofuscinosis capture distinct effects of TPP1 and CLN3 mutations on the endocytic pathway (2014)
    Oxford University Press
    Details
    Publication Date: 2014-03-20
    Description: Neuronal ceroid lipofuscinosis (NCL) comprises ~13 genetically distinct lysosomal disorders primarily affecting the central nervous system. Here we report successful reprograming of patient fibroblasts into induced pluripotent stem cells (iPSCs) for the two most common NCL subtypes: classic late-infantile NCL, caused by TPP1(CLN2) mutation, and juvenile NCL, caused by CLN3 mutation. CLN2/TPP1- and CLN3-iPSCs displayed overlapping but distinct biochemical and morphological abnormalities within the endosomal–lysosomal system. In neuronal derivatives, further abnormalities were observed in mitochondria, Golgi and endoplasmic reticulum. While lysosomal storage was undetectable in iPSCs, progressive disease subtype-specific storage material was evident upon neural differentiation and was rescued by reintroducing the non-mutated NCL proteins. In proof-of-concept studies, we further documented differential effects of potential small molecule TPP1 activity inducers. Fenofibrate and gemfibrozil, previously reported to induce TPP1 activity in control cells, failed to increase TPP1 activity in patient iPSC-derived neural progenitor cells. Conversely, nonsense suppression by PTC124 resulted in both an increase of TPP1 activity and attenuation of neuropathology in patient iPSC-derived neural progenitor cells. This study therefore documents the high value of this powerful new set of tools for improved drug screening and for investigating early mechanisms driving NCL pathogenesis.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
    Published by Oxford University Press
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