ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Gibberellin  (10)
  • Springer  (10)
  • National Academy of Sciences
  • 2010-2014
  • 1975-1979  (10)
Collection
Keywords
Publisher
  • Springer  (10)
  • National Academy of Sciences
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Gibberellin metabolism in excised lettuce hypocotyls: Evidence for the formation of gibberellin A1 glucosyl conjugates (1977)
    Springer
    Planta 136 (1977), S. 261-269 
    Details
    ISSN: 1432-2048
    Keywords: Conjugates ; Gibberellin ; Lactuca ; Metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The properties of the water-soluble metabolites of [3H]gibberellin A1 ([3H]GA1) from lettuce (Lactuca sativa L.) hypocotyls were compared with those of authentic samples of gibberellin (GA) glucosyl esters and ethers. Partitioning against l-butanol at high and low pH was not an efficient method of differentiating between ester and ether conjugates of GA1 or GA3. Extraction into l-butanol at pH 2.5 was, however, useful as a group purification step. Gel-filtration on acrylamide indicated a mean molecular weight of ca. 600 for the polar material and high-voltage electrophoresis separated two compounds (LH 1 and LH 2) with differing charge properties. Both metabolites incorporated 14C from glucose and 3H from GA1. Subsequent enzymatic hydrolysis of LH 1 released material with identical properties to [14C]glucose together with a second uncharacterised component. Feeding with [3H]GA1 methyl ester greatly reduced the formation of LH 1 but not LH 2. The metabolites were provisionally identified as GA1-glucosyl ester (LH 1) and GA1-glucosyl ether (LH 2).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385994
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Temperature dependence of the gibberellin response in lettuce hypocotyls (1978)
    Springer
    Planta 141 (1978), S. 283-288 
    Details
    ISSN: 1432-2048
    Keywords: Gibberellin ; Growth-kinetics ; Lactuca ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between temperature and sensitivity to gibberellin A3 (GA3) was studied in lettuce seedlings (Lactuca sativa L. cv. Arctic). Dose/response curves for hypocotyl elongation (10-4 mol l-1 to 10-8 mol l-1) were constructed for a range of temperatures and the slope of the linear portion of the plots used as an indication of the sensitivity to GA3. Hypocotyls were unresponsive to GA3 below 13°C but above this temperature sensitivity increased linearly. Plots of growth rate against temperature had inflexions between 12°C and 13°C, with slopes above this point which increased with increasing GA3 concentration. The Q10 value for response increased in a similar manner. Reaction rates of NAD-dependent malate dehydrogenase and peroxidase extracted from hypocotyls varied linearly with temperature whilst nonspecific tetrazolium reduction, a membrane based activity, showed an abrupt rate change above 14°C. Pre-exposure to GA3 had no effect on the temperature responses of soluble or particulate enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388345
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The role of acidification in gibberellic acid- and fusicoccin-induced elongation growth of lettuce hypocotyl sections (1978)
    Springer
    Planta 142 (1978), S. 135-145 
    Details
    ISSN: 1432-2048
    Keywords: Acid growth ; Cell elongation ; Gibberellin ; Fusicoccin ; Lactuca ; Hordeum ; Potasium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The roles of gibberellic acid (GA3) and fusicoccin (FC) in the elongation growth and acidification of the medium by excised hypocotyl sections of lettuce (Lactuca sativa L.) were investigated. Hypocotyl sections incubated in buffer without GA3 elongate optimally at pH 4.0–4.25 while sections incubated with GA3 show the same growth between pH 4.25 and 6.0. Preincubation of sections at pH 6.0 for 6 h does not affect the subsequent elongation response to acidic medium (pH 4.25); however, the sections become refractory to further acid treatment after their initial burst of growth in response to pH 4.25. Sections made refractory to acid are responsive to GA3 application, however, and the rate of growth in response to GA3 of sections pretreated for 6 h at pH 4.25 is 85% of that of sections pretreated at pH 6.0. Although preincubation of sections for 48 h in medium at pH 6.0 abolishes the GA3 response, it does not affect the response to buffer at pH 4.25. FC stimulates elongation growth in letuce hypocotyls at an optimal concentration of 1 μM, and pretreatment of sections at pH 4.25 does not affect this elongation response. Although both GA3 and FC increase elongation of the section, neither causes appreciable acidification of the medium. Addition of KCl or NaCl to FC-treated sections causes rapid medium acidification but addition of salts to GA3-treated tissue does not cause acidification. Abrasion of the hypocotyl to remove the cuticle does not enhance acidification of the medium by the sections nor deos it affect elongation of the sections in response to GA3 or FC. Medium acidification by the sections is not a passive process since it is abolished both by low temperature (2° C) and metabolic inhibitors (carbonyl cyanide-m-chlorophenyl-hydrazone, azide). The acidification of the medium by barley (Hordeum vulgare L.) roots in response to FC is also dependent on the presence of KCl. We conclude that the acid-growth hypothesis does not explain GA3- or FC-induced elongation in lettuce hypocotyls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388204
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Interaction of [3H]gibberellin A1 with a sub-cellular fraction from lettuce (Lactuca sativa L.) hypocotyls (1979)
    Springer
    Planta 146 (1979), S. 353-361 
    Details
    ISSN: 1432-2048
    Keywords: Gibberellin ; Lactuca
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of [3H]gibberellin A1 ([3H]GA1) amongst sub-cellular fractions from lettuce (Lactuca sativa L.) hypocotyl sections was examined by differential centrifugation. Material pelleting at 2000 g (2KP) accounted for 2–5% of the total tissue radioactivity and no other significant interactions were detected. After rigorous washing procedures, the 2KP fraction was found to consist mainly of cell wall material and the radioactivity remained associated during sucrose density-gradient centrifugation. 2KP-labelling increased linearly with time and was only partially removed by chasing for up to 40 h in the presence of unlabelled GA1. A linear plot was obtained for the relationship between external concentration and the log of 2KP GA1 content with a maximum percentage incorporation at 10-5 mol 1-1. Incorporation of radioactivity was reduced by chasing with unlabelled GA1 or GA9, but not by GA8. Labelling of the 2KP fraction increased uniformly with temperature between 15°C and 30°C but showed little change below 15°C. Growth rate and 2KP labelling were highly correlated (r=0.989). The possible significance of these findings is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387808
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Interaction of [3H] gibberellin A1 with a sub-cellular fraction from lettuce (Lactuca sativa L.) hypocotyls (1979)
    Springer
    Planta 146 (1979), S. 363-368 
    Details
    ISSN: 1432-2048
    Keywords: Cell walls ; Gibberellin ; Growth ; Lactuca
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Radioactive 2,000 g pellets (2KP) derived from lettuce (Lactuca sativa) hypocotyl sections incubated with [3H]gibberellin A1 ([3H]GA1) were subjected to a range of treatments to test the stability of labelling. The labelled association was stable in 0.1 M buffer at pH 3 and pH 9 and also in 1 M salt, organic solvents, protease or cellulase. Both 1 M potassium hydroxide (KOH) and a quaternary ammonium hydroxide tissue solubiliser effected an 80% release of incorporated [3H]. No radioactivity was released during polyacrylamide gel electrophoresis. Gel chromatography of KOH digests indicated the presence of labelled compounds with a higher molecular weight than GA1. This material remained near to the origin during high-voltage paper electrophoresis. Calculations based upon KOH extraction of 2KP material suggested that 5 to 20% of the total uptake of [3H]GA1 could be recovered from this fraction. The implications of these findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387809
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Interaction of [3H]gibberellin A1 with a sub-cellular fraction from lettuce (Lactuca sativa L.) hypocotyls (1979)
    Springer
    Planta 147 (1979), S. 264-268 
    Details
    ISSN: 1432-2048
    Keywords: Gibberellin ; Growth ; Lactuca ; Protein-synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between protein synthesis and the incorporation of [3H]gibberellin A1 ([3H]GA1) into a 2,000xg pelletable (2KP) fraction from lettuce (Lactuca sativa L.) hypocotyl sections has been investigated. Concentrations of D-2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide (MDMP) between 10-7 M and 10-4 M caused increasing inhibition of growth, 2KP labelling and incorporation of [14C]leucine into soluble protein. Growth and 2KP radioactivity were highly correlated (r=0.996). Transfer to MDMP early or late in the course of GA response caused reductions in both growth and incorporation into the 2KP fraction. Exposure to the inhibitor had more effect at 4 h than at 20 h. The proportions of alkali-soluble and insoluble radioactivity in the 2KP fraction were also altered by this treatment. The implications of these findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388749
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Effects of colchicine and lumicolchicine on hypocotyl elongation, respiration rates and microtubules in gibberellic-acid-treated lettuce seedlings (1978)
    Springer
    Planta 139 (1978), S. 301-303 
    Details
    ISSN: 1432-2048
    Keywords: Cell elongation ; Colchicine ; Gibberellin ; Lumicolchicine ; Microtubules ; Respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gibberellic-acid (GA3)-induced hypocotyl elongation of intact lettuce (Lactuca sativa L.) seedlings was inhibited by colchicine (4×10-4 M) but not by lumicolchicine (4×10-4 M). In excised lettuce hypocotyls, GA3 (10-5 M) increased respiration over water controls, while both colchicine and lumicolchicine alone, or in combination with GA3, reduced respiration. Microtubules were present in the hypocotyls of lumicolchicine-treated seedlings but absent in those treated with colchicine. It is suggested that lumicolchicine is a useful drug to discriminate between the metabolic and microtubule-mediated processes in cell morphogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388644
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    The biological activities of some new gibberellins (GAs) in six plant bioassays (1977)
    Springer
    Planta 135 (1977), S. 143-147 
    Details
    ISSN: 1432-2048
    Keywords: Bioassays ; Gibberellin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biological activities of GA40, GA43, GA46, GA47, GA51 and GA4 20,4-lactone were tested over a wide range of concentrations in six plant bioassays. GA4 20,4-lactone showed the highest activity. Of the two 2α-hydroxylated compounds GA47 showed moderately high activity, and GA40 was slightly active. The 2β-hydroxylated compunds GA43, GA46 and GA51 were virtually inactive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387163
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Cell elongation and cell division in elongating lettuce hypocotyl sections (1977)
    Springer
    Planta 135 (1977), S. 249-255 
    Details
    ISSN: 1432-2048
    Keywords: Cell division ; Cell elongation ; Gibberellin ; Lactuca ; Potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The roles of cell division and cell elongation in the growth of sections excised from hypocotyls of lettuce (Lactuca sativa L. cv. Arctic) were investigated. Elongation of sections incubated in the light is inhibited compared to dark-grown sections and this inhibition is reversed by gibberellic acid (GA3). The elongation of both dark-grown and GA3-treated, light-grown sections can be enhanced by 10mM KCl. Under all conditions of incubation, elongation growth is greatest in the uppermost quarter of the hypocotyl section while the basal quarter does not elongate. In darkness the two apical segments of sections marked into four equal parts grow at the same rate, while in light, growth of the apical segment exceeds that of the second segment. Cell division in cortical or epidermal cells, as measured by mitotic index or cell number, is not affected by illumination conditions nor by GA3 or KCl treatments. Although γ-irradiation and FUDR pretreatment eliminate or cause a marked reduction in cell division in the excised hypocotyl, sections from seeds irradiated with γ-rays or incubated in 5-fluorodeoxyuridine elongate in response to GA3 and KCl treatment as do sections from non-pretreated controls. Therefore, since neither GA3 nor darkness affect celldivision activity and since treatments which eliminate or significantly reduce cell division do not affect growth, we conclude that the effect of GA3 and darkness in this material is to increase cell elongation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384897
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Effect of hormones on nucleolar growth and vacuolation in elongating cotton fibers (1978)
    Springer
    Planta 140 (1978), S. 269-273 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Auxin Cell elongation ; Fibers ; Gibberellin ; Gossypium ; Nucleolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rather precise combination of the three phytohormones, gibberellic acid, auxin, and abscisic acid, is necessary for the considerable growth of fiber nucleoli in Gossypium hirsutum L. for about 8 days after anthesis and for nucleolar “vacuolation” in the second half of that period. Nucleolar growth and vacuolation must occur in a precise sequence for the fiber to reach a good final length. Nucleolar vacuolation indicates simultaneous output and neosynthesis of nucleolar material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390259
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...