ISSN:
0148-7280
Keywords:
chromosome
;
spermatozoa
;
sperm
;
egg
;
zona-free
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
An improved method for human sperm chromosome preparation is described. Improvements include (1) the use of a sperm population with high motility and normal morphology for insemination, (2) the insemination and postinsemination culture of zona-free eggs in Holmes medium, which does not require serum supplementation, (3) control of sperm concentration at insemination to avoid heavy polyspermy, (4) reduction of the occurrence of egg agglutination by placing the medium for postinsemination culture in a ring or crescent shape instead of a droplet, and (5) application of a two-step fixation method to augment the efficiency of chromosome preparation.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120100303
Permalink