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  • 1
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Adult male marbled newts (Triturus marmoratus) were collected at the beginning of the spermatogenetic period and exposed to different photoperiods (natural photoperiod with progressively increasing daylengths, total darkness, 8L:16D, 12L:12D, 16L:8D, and continuous light) for 3 months at 20°C. To evaluate the effect of photoperiodic input via pineal gland photoreceptors, two additional groups of newts were blinded by a non-aggressive method (an elastic rubber cap was adjusted to the head to cover the eyes but not the pineal photoreceptors). These animals were exposed either to the natural photoperiod or to 12 hr of light per day. Quantitative histologic studies on testicular development and germ-cell volume revealed no significant differences between non-blinded and blinded animals. Testicular size and germ-cell development increased in the following order: total darkness, constant light, 8L:12D, natural photoperiod, 12L:12D, and 16L:8D. These results suggest that (1) long photoperiods enhance testicular development, whereas short photoperiods or an environment of continuous light have the opposite effects and (2) the effect of photoperiods on testicular function in newts is independent of the ocular photoreceptors.
    Additional Material: 16 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 32 (1988), S. 379-385 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Simple algebraic expressions for the flux of substrate into a steady-state biofilm are developed. This pseudoanalytical solution, which eliminates the need for repetitiously solving numerically a set of nonlinear differential equations, is based on an analysis of the numerical results from the numerical solution of the differential equations. The critical advantage of this new pseudoanalytical solution is that it is highly accurate for the entire range of substrate concentrations and kinetic parameters. The article also illustrates that previous pseudoanalytical solutions for steady-state biofilm kinetics are seriously inaccurate for certain ranges of substrate concentration and kinetic parameters.
    Additional Material: 7 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 32 (1988), S. 341-344 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some properties of the cellulolytic complex obtained from Trichoderma reesei QM 9414 grown on Solka floc as carbon source and its ability to hydrolyze the lignocellulosic biomass of Onopordum nervosum Boiss were studied. The optimum enzyme activity was found at temperatures between 50 and 55°C and pH ranging from 4.3 to 4.8. Hydrolysis of 4-nitropnenyl-β-D-glucopyranoside (4-NPG) and cellobiose by the β-glucosidase of the complex, showed competitive inhibition by glucose with a Ki value of 0.8 mM for 4-NPG and 2. 56 mM for cellobiose. Enzymatic hydrolysis yield of Onopordum nervosum, evaluated as glucose production after 48 h, showed a threefold increase by pretreating the lignocellulosic substrate with alkali. When the loss of glucose incurred by de pretreatment was taken into account, a 160% increase in the final cellulose to glucose conversion was found to be due to the pretreatment.
    Additional Material: 5 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 32 (1986), S. 353-368 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydrodynamics of trickling flow in packed beds is modeled by representing the porous medium as an array of parallel conduits of circular cross section.First, a straight tube model is developed and analytical solutions are obtained for the relative permeabilities of the gas and liquid phases. Then a periodically constricted tube model is proposed and the equations of motion are solved numerically to determine the effect that surface tension forces have on the relative permeabilities. The constricted tube model predicts that the relative permeabilities of the phases are appreciably sensitive to surface tension forces, a prediction that seems at odds with experimental observations. This discrepancy may be caused by the assumption of fully wetted surface area of particles employed in the model.The straight tube model confirms experimental results indicating that the liquid phase relative permeability is, for practical purposes, insensitive to the gas flow rate and to the gas-to-liquid density and viscosity ratios. Both conduit models show that the gas phase relative permeability curves are strong functions of the gas phase Reynolds number when this parameter is small. For large gas Reynolds numbers, a single curve for the relative permeability as a function of saturation is obtained. These trends are observed in previous experimental studies.
    Additional Material: 15 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 32 (1986), S. 369-380 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The liquid holdup and the pressure drop for two-phase trickling flow in a packed bed were measured experimentally. Different values of those parameters were obtained as the liquid flow rate was increased and then decreased, indicating a multiplicity in hydrodynamic states. This behavior was observed even when the gas flow rate was zero in the bed. It was determined that the hysteresis exhibited by the process is due to imperfect wetting of the packing and to the difference between advancing and receding contact angles at the gas-liquid-solid contact lines. The reduced wetting conditions are also the cause of the increase in liquid holdup observed when the surface tension of the liquid is decreased. In this case, the amount of liquid retained in the bed is controlled by the extension of the wetted regions due to the more favorable contact angles as the surface tension decreases. The transition from the trickling to the pulsing flow regime was also dependent on the history of the process.A new correlation for predicting liquid holdups and pressure drops for trickling flow in packed beds is proposed for the decreasing liquid flow rate operating mode. This correlation is based on the experimental determination of the liquid phase relative permeability as a function of the the liquid phase reduced saturation and the determination of the gas phase relative permeability as a function of the gas phase saturation and the gas phase Reynolds number. The new data are analyzed in the light of the theory developed in the first part of this paper.
    Additional Material: 15 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 31 (1985), S. 52-62 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydrodynamics of cocurrent gas-liquid flow in packed beds is analyzed by extending the concept of relative permeability to the inertial regime.The relative permeabilities of the gas and liquid phases are functions of the saturation of the liquid phase. These functions are found from an analysis of experimental data. The relations obtained are used to develop empirical correlations for predicting liquid holdup and pressure drop in gas-liquid cocurrent downflow in packed beds over a wide range of operating conditions. The correlations proposed give very good results when compared to experimental data yielding, in general, mean relative deviations lower than existing correlations. In addition, a new equation is proposed for predicting static holdup in packed beds which is based on a more physically realistic characteristic length than that used in previous studies.
    Additional Material: 8 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 129 (1986), S. 257-263 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have investigated the effects of insulin and somatomedin-C/insulinlike growth factor l(Sm-C) in purified porcine Leydig cells in vitro on gonadotrophins (hCG) receptor number, hCG responsiveness (cAMP and testosterone production), and thymidine incorporation into DNA. Leydig cells cultured in a serum-free medium containing transferrin, vitamin E, and insulin (5 μ/ml) maintained fairly constant both hCG receptors and hCG responsiveness. When they were cultured for 3 days in the same medium without insulin, there was a dramatic decline (more than 80%) in both hCG receptor number and hCG responsiveness. However the cAMP but not the testosterone response to forskolin was normal. Both insulin and Sm-C at nanomolar concentrations prevent the decline of both hCG receptors and hCG-induced cAMP production. This effect of both peptides was dose dependent with an ED50 of about 1 ng/ml and 5 ng/ml for SM-C and insulin, respectively. Insulin and Sm-C had no additive effect on these parameters. At nanomolar concentrations, Sm-C and insulin enhanced hCG-induced testosterone production but the effect of Sm-C was significantly higher than that of insulin. However, the effect of insulin at higher concentrations (5 μg/ml) was significantly higher than that of Sm-C at 50 ng/ml. In contrast, at nanomolar concentrations only Sm-C stimulated [3H]-thymidine incorporation into DNA and cell multiplication, the stimulatory effect of insulin on these parameters, was seen only at micromolar concentrations. These results indicate that both Sm-C and insulin acting through their own receptors increase Leydig cell steroidogenic responsiveness to hCG by increasing hCG receptor number and improving some step beyond cAMP formation. ln contrast, the mitogenic effects of insulin are mediated only through Sm-C receptors.
    Additional Material: 6 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 5 (1985), S. 601-614 
    ISSN: 0271-2091
    Keywords: Finite Elements ; Stokes Equations ; Periodically Constricted Tubes ; Quadrilateral Elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The use of the finite element method in solving the problem of flow of a Newtonian fluid in periodically constricted tubes is explored. The performance of eight node serendipity and nine node Lagrangian elements is compared. It was found that the Lagrangian element results in unstable velocity fields when stagnant or recirculation regions are present. This is characteristic of tubes with large expansion zones. The eight node element does not exhibit instabilities. Both elements give accurate pressure fields. This behaviour is contrary to traditional results obtained for flow problems with similar geometrical characteristics. This suggests that the periodicity of the boundary conditions might be the cause of the instabilities in the numerical solution.The use of the continuity equation to simplify the viscous terms in the Stokes equations resulted, in this particular case, in a deterioration of the rate of convergence of the algorithm.
    Additional Material: 11 Ill.
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