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  • Freeze substitution  (2)
  • Solar Physics  (2)
  • Fungal adhesion
  • 2010-2014  (2)
  • 1990-1994  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 163 (1991), S. 199-202 
    ISSN: 1615-6102
    Keywords: Actin ; Freeze substitution ; Fungi ; Hyphal tip ; Immunocytochemistry ; Magnaporthe grisea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have successfully localized fungal actin for the first time using immuno-electron microscopy and hyphal tips of the rice blast pathogenMagnaporthe grisea. Following ultrarapid freezing, samples were processed in a novel substitution fluid of 10% acrolein in anhydrous ethanol and embedded in LR White resin. A monoclonal anti-actin antibody, previously shown to recognizeM. grisea actin, bound specifically to filasomes concentrated in the peripheral cytoplasm of subapical regions, and to the core-region of the Spitzenkörper.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 181 (1994), S. 202-212 
    ISSN: 1615-6102
    Keywords: Fungal adhesion ; Appressoria ; Plant disease ; Cochliobolus heterostrophus ; Magnaporthe grisea ; Nectria haematococca ; Uromyces spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Firm adhesion of fungal plant pathogens to their hosts is critical at several stages in the host-parasite interaction. Spores of many fungal species are capable of rapid, non-specific attachment to various surfaces. This early adhesion, which often occurs well before germ tube emergence, prevents spores from being blown or washed from the host surface before infection can take place. Adhesion is critical for proper sensing of topographic signals involved in thigmotropic responses and for differentiation and function of appressoria. Four fungal pathogens which exhibit a variety of adhesion mechanisms have been selected for discussion.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 168 (1992), S. 20-26 
    ISSN: 1615-6102
    Keywords: Appressoria ; Freeze substitution ; Immunofluorescence ; Plant disease ; Phalloidin ; Pyricularia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The penetration peg is the structure used byMagnaporthe grisea to pierce the surface of rice leaves or very hard nonbiodegradable substrates. Penetration pegs produced by appressoria in vitro were examined by electron microscopy and immunofluorescence microscopy using various fluorophore labeled anti-actins. Freeze-substitution preparation of appressoria at early stages of substrate penetration showed that peg cytoplasm consisted primarily of a zone of exclusion, excluding even ribosomes, and was continuous with a similar region in the appressorium. Apical vesicles were, however, observed in short, presumably elongating pegs. Immunofluorescence microscopy was used to demonstrate binding of a monoclonal anti-actin to penetration peg cytoplasm, following “permeabilization” of appressoria by means of a brief sonication. Occasional filaments and ca. 300 nm diameter plaques were labeled in appressorial cytoplasm. Western blot analysis of germ tube extracts showed that the monoclonal probe bound predominantly to a single band with a molecular weight similar to that of rabbit muscle actin. Preincubation of the antibody with actin virtually eliminated peg labeling. We conclude that the penetration peg contains actin which may play a role in the formation of the zone of exclusion.
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  • 4
    Publication Date: 2019-07-13
    Description: We report on the radio-emission characteristics of 222 interplanetary (IP) shocks detected by spacecraft at Sun-Earth L1 during solar cycle 23 (1996 to 2006, inclusive). A surprisingly large fraction of the IP shocks (approximately 34%) was radio quiet (RQ; i.e., the shocks lacked type II radio bursts). We examined the properties of coronal mass ejections (CMEs) and soft X-ray flares associated with such RQ shocks and compared them with those of the radio-loud (RL) shocks. The CMEs associated with the RQ shocks were generally slow (average speed approximately 535 km/s) and only approximately 40% of the CMEs were halos. The corresponding numbers for CMEs associated with RL shocks were 1237 km/s and 72%, respectively. Thus, the CME kinetic energy seems to be the deciding factor in the radio-emission properties of shocks. The lower kinetic energy of CMEs associated with RQ shocks is also suggested by the lower peak soft X-ray flux of the associated flares (C3.4 versus M4.7 for RL shocks). CMEs associated with RQ CMEs were generally accelerating within the coronagraph field of view (average acceleration approximately +6.8 m/s (exp 2)), while those associated with RL shocks were decelerating (average acceleration approximately 3.5 m/s (exp 2)). This suggests that many of the RQ shocks formed at large distances from the Sun, typically beyond 10 Rs, consistent with the absence of metric and decameter-hectometric (DH) type II radio bursts. A small fraction of RL shocks had type II radio emission solely in the kilometric (km) wavelength domain. Interestingly, the kinematics of the CMEs associated with the km type II bursts is similar to those of RQ shocks, except that the former are slightly more energetic. Comparison of the shock Mach numbers at 1 AU shows that the RQ shocks are mostly subcritical, suggesting that they were not efficient in accelerating electrons. The Mach number values also indicate that most of these are quasi-perpendicular shocks. The radio-quietness is predominant in the rise phase and decreases through the maximum and declining phases of solar cycle 23. About 18% of the IP shocks do not have discernible ejecta behind them. These shocks are due to CMEs moving at large angles from the Sun-Earth line and hence are not blast waves. The solar sources of the shock-driving CMEs follow the sunspot butterfly diagram, consistent with the higher-energy requirement for driving shocks.
    Keywords: Solar Physics
    Type: GSFC.JA.4549.2011 , The Astrophysical Journal; 710; 1111-1126
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  • 5
    Publication Date: 2019-07-19
    Description: The excellent correspondence between type II radio bursts and solar energetic particles (SEPs) made it clear that the same shock accelerates ions and electrons. A recent investigation involving a large number of I shocks revealed that about 35% of IP shocks do not produce type II bursts (radio quiet) or SEPs. Comparing the RQ shocks with the radio loud (RL) ones revealed some interesting results, which will be summarized in this poster. (1) There is no evidence for blast waves. (2) Even a small fraction (20%) of RQ shocks is associated with ion enhancements at the shock when the shock passes the spacecraft. (3) The primary difference between the RQ and RL shocks can be traced to the different kinematic properties of the associated CMEs, although the shock properties measured at 1 AU are not too different for the RQ and RL cases. This can be attributed to the interaction with the IP medium, which seems to erase the difference. More details can be found in Astrophysical Journal 710, 1111, 2010 (http://adsabs.harvard.edu/absZ2009arXivO9l2.4719G).
    Keywords: Solar Physics
    Type: Solar Heliospheric and Interplanetary Environment (SHINE); Jul 26, 2010 - Jul 30, 2010; Santa Fe, New Mexico; United States
    Format: text
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