ISSN:
1573-5044
Keywords:
Ajmalicine
;
alkaloids
;
catharanthine
;
Catharanthus roseus
;
hairy roots
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two year old, transformed root cultures of Catharanthus roseus accumulate ajmalicine and catharanthine (0.57 and 0.36 mg g-1 DW, or 7.0 and 3.0 mg l-1, respectively). Changes in the concentration of the medium components, as well as the addition of hydrolytic enzymes and biotic elicitors, were used as strategies to increase these alkaloid yields. Regarding the components of the medium, the results obtained, when sucrose was raised from 3 to 4.5%, are noteworthy. The nitrogen source induced differential responses in the individual alkaloid yields. No net change in the alkaloid content was observed either with changes in the concentration of vitamins or macro-and micronutrients. Though the root culture only shows a limited response to elicitors, Aspergillus treatment and the use of macerozyme increased the accumulation of ajmalicine selectively, while the addition of methyl jasmonate increased the yield of both alkaloids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033887
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