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  • Nitrogen fixation  (2)
  • crop evolution  (2)
  • Springer  (4)
  • American Society of Hematology
  • Springer Nature
  • 2010-2014
  • 1990-1994  (4)
  • 1935-1939
Collection
Publisher
  • Springer  (4)
  • American Society of Hematology
  • Springer Nature
Years
Year
  • 1
    ISSN: 1617-4623
    Keywords: Nitrogen fixation ; Novel type of NifU ; Ethane formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequence analysis of a 3494-bp HindIII-Bc1I fragment of the Rhodobacter capsulatus nif region A revealed genes that are homologous to ORF6, nifU, nifS, nifV and nifW from Azotobacter vinelandii and Klebsiella pneumoniae. R. capsulatus nifU, which is present in two copies, encodes a novel type of NifU protein. The deduced amino acid sequences of NifUI and NifUII share homology only with the C-terminal domain of NifU from A. vinelandii and K. pneurnoniae. In contrast to nifA andnifB which are almost perfectly duplicated, the predicted amino acid sequences of the two NifU proteins showed only 39% sequence identity. Expression of the ORF6-nifU ISVW operon, which is preceded by a putative σ54-dependent promoter, required the function of NifA and the nif-specific rpoN gene product encoded by nifR4. Analysis of defined insertion and deletion mutants demonstrated that only nifS was absolutely essential for nitrogen fixation in R. capsulatus. Strains carrying mutations in nifV were capable of very slow diazotrophic growth, whereas ORF6, nifU I and nifW mutants as well as a nifU I/nifUII, double mutant exhibited a Nif+ phenotype. Interestingly, R. capsulatus nifV mutants were able to reduce acetylene not only to ethylene but also to ethane under conditions preventing the expression of the alternative nitrogenase system. Homocitrate added to the growth medium repressed ethane formation and cured the NifV phenotype in R. capsulatus. Higher concentrations of homocitrate were necessary to complement the NifV phenotype of a polar nifV mutant (NifV−NifW−), indicating a possible role of NifW either in homocitrate transport or in the incorporation of this compound into the iron-molybdenum cofactor of nitrogenase.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5079
    Keywords: Anabaena ; ferredoxin ; flavodoxin ; FPLC determination of ferredoxin and flavodoxin ; Iron deficiency ; Nitrogen fixation ; Photosynthesis ; strain differences in gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Iron-dependent formation of ferredoxin and flavodoxin was determined in Anabaena ATCC 29413 and ATCC 29211 by a FPLC procedure. In the first species ferredoxin is replaced by flavodoxin at low iron levels in the vegetative cells only. In the heterocysts from Anabaena ATCC 29151, however, flavodoxin is constitutively formed regardless of the iron supply. Replacement of ferredoxin by flavodoxin had no effect on photosynthetic electron transport, whereas nitrogen fixation was decreased under low iron conditions. As ferredoxin and flavodoxin exhibited the same Km values as electron donors to nitrogenase, an iron-limited synthesis of active nitrogenase was assumed as the reason for inhibited nitrogen fixation. Anabaena ATCC 29211 generally lacks the potential to synthesize flavodoxin. Under iron-starvation conditions, ferredoxin synthesis is limited, with a negative effect on photosynthetic oxygen evolution.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 40 (1993), S. 143-151 
    ISSN: 1573-5109
    Keywords: electrophoresis ; crop evolution ; faba bean ; geographical isolation ; germplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty-six faba bean (Vicia faba L.) populations, from five geographic areas and five varietal types have been assayed electrophoretically for variation in seven isozyme systems. Isozyme frequencies in populations, botanical varieties and geographical areas were submitted to the principal component analysis (PCA). Varieties grouped together showed no differences among them except for isolated populations. Populations from Ethiopia and China lay far apart in PCA from those of other origins. It seems that no genetic divergence at the isozyme level took place during the varietal formation and dispersion process, except when geographical isolation of the population occurred. The results support the hypothesis of an isolation of the crop in Ethiopia and in China.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 40 (1993), S. 105-111 
    ISSN: 1573-5109
    Keywords: crop evolution ; discriminate analysis ; germplasm ; landraces ; principal component analysis ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Univariate and multivariate analysis were used to examine phenotypic variability within a Spanish faba bean germplasm collection maintained at the Centro de Investigación y Desarrollo Agrario, Córdoba, Spain. The analysis of 158 Spanish faba bean accessions and 12 quantitative traits indicated highly significant differences among botanical groups for 8 characters and among geographic regions for 10 characters. An east to west clinal pattern of variation for some characters was detected. In order to identify the main characters which account for the major variation, the same collection was subjected to principal component analysis for 12 quantitative traits. Reproductive and plant height characters appeared to be the major sources of diversity. To determine the importance of both geographic and botanical variation among the Spanish cultivars, discriminant analysis was applied. According to these analyses, plant height, height of the lowest pod-bearing node, pod length and 100 seed weight, were important traits discriminating among different geographic regions. The main character discriminating among botanical groups was the 100 seed weight. Our results fit in a pattern in which both agroecological and anthropological causes could have played a role in the observed variation. This analysis can help plant breeders in choosing the most favorable accessions in plant breeding.
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