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  • Bacillus thuringiensis  (1)
  • dilute polystyrene solution
  • Springer  (2)
  • Oxford University Press
  • 2010-2014
  • 1990-1994  (2)
  • 1960-1964
Collection
Publisher
  • Springer  (2)
  • Oxford University Press
Years
  • 2010-2014
  • 1990-1994  (2)
  • 1960-1964
Year
  • 1
    ISSN: 1573-5028
    Keywords: Bacillus thuringiensis ; cry genes ; insecticidal crystal protein ; protoplast electroporation ; RNA stability ; transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined expression of several insecticidal crystal protein (ICP) genes of Bacillus thuringiensis in transgenic tobacco plants and electroporated carrot protoplasts. We determined that low levels of lepidopteran toxin cryIA(b) ICP gene expression in plants and electroporated carrot cells is due to RNA instability. We used a series of 3′ deleted cryIA(b) constructs directed by the cauliflower mosaic virus 35S promoter to demonstrate that this instability is minimally contained in the first 579 bases of the gene in both systems. This instability may result from 5′ → 3′ as well as 3′ → 5′ RNA metabolism. The coleopteran toxic cryIIIA gene was also examined in electroporated carrot cells, and found to be poorly expressed. A model for improvement of ICP RNA stability in plants is presented.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Rheologica acta 31 (1992), S. 274-282 
    ISSN: 1435-1528
    Keywords: Light-scattering ; flowing polymer solution ; dilute polystyrene solution ; Taylor-vortex ; solvent power
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Abstract Rheological measurements and light-scattering experiments were performed on dilute solutions of high molecular polystyrene. We are able to describe the orientation behavior of chain molecules under shear flow by means of light-scattering. Beyond that these investigations of light-scattering of flowing polymer solutions are an useful and suitable tool for detection and characterization of Taylor vortex formation. We can estimate the appearance of these hydrodynamic instabilities, which overlay the laminar main flow and we can observe a typical influence of the solvent power on it.
    Type of Medium: Electronic Resource
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