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  • Turbulence  (3)
  • glycoprotein  (2)
  • American Geophysical Union  (3)
  • Springer  (2)
  • Annual Reviews
  • Paleontological Society
  • 2010-2014  (3)
  • 1990-1994  (2)
  • 1970-1974
  • 1935-1939
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  • 1
    ISSN: 1573-4986
    Keywords: Friend spleen focus-forming virus ; glycoprotein ; oligosaccharide processing ; SFFV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Normal rat kidney cells, non-productively infected with the anaemia-inducing variant of Friend spleen focus-forming virus (F-SFFVA), were metabolically labelled with [2-3H]mannose. The primary translation product of the viral envelope gene (env), representing a glycoprotein with an apparent molecularM r of 55 000 (gp55), was isolated from cell lysates by immunoaffinity chromatography and purified by preparative SDS/PAGE. Radiolabelled oligosaccharides, released from tryptic glycopeptides by treatment with endo-β-N-acetylglucosaminidase H, were characterized chromatographically, by enzymic digestion and by acetolysis. The results revealed that F-SFFVA gp55 obtained from this source carried predominantly oligomannose type sugar chains with five to nine mannoses. As a characteristic feature, glycans with seven to nine mannoses contained, in part, an additional glucose residue. Although the amount of glucosylated species found was higher in F-SFFVA gp55 (about 25% of total endo-H-sensitive oligosaccharides) than in gp55 of the corresponding polycythaemia-inducing variant (F-SFFVP, 16.3%), the overall glycosylation pattern of the F-SFFVA env product closely resembled that of F-SFFVP gp55 [Strubeet al. (1988)J Biol Chem 263:3762–71]. Hence, our results demonstrate that the different intracellular processing and transport of the primary F-SFFVA env product cannot be attributed to aberrant trimming of its oligomannose type glycans.
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  • 2
    ISSN: 1573-4986
    Keywords: glycoprotein ; glycopeptides ; N-linked oligosaccharides ; snake venom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In a previous study, we determined the structures of the glycans present in ancrod, a thrombin-like serine protease from the venom of the Malayan pit viperAgkistrodon rhodostoma (Pfeifferet al. (1992)Eur J Biochem 205:961–78). In order to allocate the various carbohydrate chains to distinctN-glycosylation sites of the molecule, we have now isolated individual glycopeptides. Peptide moieties were identified after deglycosylation with peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase F by amino acid analysis and sequencing. Liberated oligosaccharides were assigned to the previously deduced carbohydrate structures by high performance liquid chromatography. Although only quantitative differences were observed, the results indicate that each glycosylation site of ancrod carries its characteristic oligosaccharide pattern. Furthermore, all potential sites were shown to be substituted by carbohydrates.
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  • 3
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2004. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 109 (2004): C05004, doi:10.1029/2003JC002094.
    Description: Rates of turbulent kinetic energy (TKE) production and buoyancy flux in the region immediately seaward (~1 km) of a highly stratified estuarine front at the mouth of the Fraser River (British Columbia, Canada) are calculated using a control volume approach. The calculations are based on field data obtained from shipboard instrumentation, specifically velocity data from a ship mounted acoustic Doppler current profiler (ADCP), and salinity data from a towed conductivity-temperature-depth (CTD) unit. The results allow for the calculation of vertical velocities in the water column, and the total vertical transport of salt and momentum. The vertical turbulent transport quantities (inline equation, inline equation) can then be estimated as the difference between the total transport and the advective transport. Estimated production is on the order of 10−3 m2 s−3, yielding a value of ɛ(νN2)−1 on the order of 104. This rate of TKE production is at the upper limit of reported values for ocean and coastal environments. Flux Richardson numbers in this highly energetic system generally range from 0.15 to 0.2, with most mixing occurring at gradient Richardson numbers slightly less than inline equation. These values compare favorably with other values in the literature that are associated with turbulence observations from regimes characterized by scales several orders of magnitude smaller than are present in the Fraser River.
    Description: This work was performed as a part of D. MacDonald’s Ph.D. thesis, and was funded by Office of Naval Research grants N000-14-97-10134 and N000-14-97- 10566, National Science Foundation grant OCE-9906787, a National Science Foundation graduate fellowship, and support from the WHOI Academic Programs Office.
    Keywords: Turbulence ; Entrainment ; Estuary
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 4
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2010. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 37 (2010): L22607, doi:10.1029/2010GL045272.
    Description: Shear instability is the dominant mechanism for converting fluid motion to mixing in the stratified ocean and atmosphere. The transition to turbulence has been well characterized in laboratory settings and numerical simulations at moderate Reynolds number—it involves “rolling up”, i.e., overturning of the density structure within the cores of the instabilities. In contrast, measurements in an energetic estuarine shear zone reveal that the mixing induced by shear instability at high Reynolds number does not primarily occur by overturning in the cores; rather it results from secondary shear instabilities within the zones of intensified shear separating the cores. This regime is not likely to be observed in the relatively low Reynolds number flows of the laboratory or in direct numerical simulations, but it is likely a common occurrence in the ocean and atmosphere.
    Description: This research was supported by NSF grant OCE‐0824871 and ONR grant N00014‐0810495.
    Keywords: Stratification ; Turbulence ; Mixing
    Repository Name: Woods Hole Open Access Server
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  • 5
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2010. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 115 (2010): C12024, doi:10.1029/2009JC006061.
    Description: Turbulent mixing of salt is examined in a shallow salt wedge estuary with strong fluvial and tidal forcing. A numerical model of the Merrimack River estuary is used to quantify turbulent stress, shear production, and buoyancy flux. Little mixing occurs during flood tides despite strong velocities because bottom boundary layer turbulence is dislocated from stratification elevated in the water column. During ebbs, bottom salinity fronts form at a series of bathymetric transitions. At the fronts, near-bottom velocity and shear stress are low, but shear, stress, and buoyancy flux are elevated at the pycnocline. Internal shear layers provide the dominant source of mixing during the early ebb. Later in the ebb, the pycnocline broadens and moves down such that boundary layer turbulence dominates mixing. Mixing occurs primarily during ebbs, with internal shear mixing accounting for about 50% of the total buoyancy flux. Both the relative contribution of internal shear mixing and the mixing efficiency increase with discharge, with bulk mixing efficiencies between 0.02 and 0.07. Buoyancy fluxes in the estuary increase with discharge up to about 400 m3 s−1 above which a majority of the mixing occurs offshore. Observed buoyancy fluxes were more consistent with the k-ɛ turbulence closure than the Mellor-Yamada closure, and more total mixing occurred in the estuary with k-ɛ. Calculated buoyancy fluxes were sensitive to horizontal grid resolution, as a lower resolution grid yielded less integrated buoyancy flux in the estuary and exported lower salinity water but likely had greater numerical mixing.
    Description: This research was funded by National Science Foundation Grant OCE‐0452054. Ralston also received support from The Penzance Endowed Fund in Support of Assistant Scientists and The John F. and Dorothy H. Magee Fund in Support of Scientific Staff at Woods Hole Oceanographic Institution.
    Keywords: Mixing ; Turbulence ; Salt wedge estuary
    Repository Name: Woods Hole Open Access Server
    Type: Article
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